Plant tissue culture: Theory and Practice, a Revised Edition

Bhojwani, Sant S.; Razdan, M. K.

doi:10.1016/s0928-3420(96)80002-4

Cited by 271 publications

(285 citation statements)

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“…The plantlets, after hardening, would be raised ex vitro to ensure a steady supply of vegetable oil from the seeds. Somaclonal variants arising from this propagation method (Bhojwani and Razdan, 1996) would include those with desirable attributes that would enhance steady availability of elite cultivars of this plant for purposes of providing a cleaner and more environmentally friendly substitute for fossil fuel and a source of commercial vegetable oil.…”

Section: Resultsmentioning

confidence: 99%

“…The superiority of Y3 to MS could be related to the quantity of ions in the basal medium. Bhojwani and Razdan (1996) showed that the main difference in the composition of a range of commonly used tissue culture media is based on the quantity of various salts and ions. Plantlets in Y3 medium irrespective of the carbon source employed showed marked difference in their root growth as compared to the ones in MS medium.…”

Section: Discussionmentioning

confidence: 99%

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Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Okafor¹,

Okezie²

2016

Afr. J. Biotechnol.

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In this study, seven carbohydrates, namely, glucose, fructose, galactose, sucrose, maltose, raffinose and starch were screened for the most suitable carbon source for the in vitro culture of oil palm (Elaeis guineensis Jacq. var. tenera) zygotic embryos on the basal media of Murashige and Skoog (MS) (1962) and Eeuwen (Y3) (1976). The embryos were excised aseptically and cultured on both media containing the different carbohydrates. Results obtained showed that while both media supported in vitro plantlet regeneration from the embryo explants, Y3 medium was significantly superior to MS medium (P=0.05) in length of root and shoot of plantlets produced. Sucrose was also significantly (P=0.05) superior to glucose and maltose while starch, raffinose, fructose and galactose had the least value in all growth parameters studied. In addition, more uniform plantlets were produced in Y3 media in which sucrose served as carbon source relative to the other six carbon sources tested. The protocol reported here has potential for speeding up germination process within a short period of time for oil palm.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Okafor¹,

Okezie²

2016

Afr. J. Biotechnol.

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“…Glucose and fructose are known to support good growth of some tissues. In general, dicotyledonous tissues grow best with sucrose whereas those of monocots do best with dextrose (glucose) (Bhojwani and Razdan, 1996). Although, sorbitol has been used for maintaining or restoring the plant regeneration ability of rice calli (Kishor and Reddy 1987;Kishor, 1987;Tsukahara and Hirosawa, 1992;Ozawa et al, 1996), supplementation of sorbitol to pre-culture media in these experiments was not much effective as maltose and sucrose alone in the media.…”

Section: Effect Of Pre-culture Media On Proliferation and Regeneratiomentioning

confidence: 85%

Comparative studies on in-vitro response of fresh and old calli of rice (Oryza sativa L.)

Amarasinghe

Yang

2006

J Agric Sciences

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In-vitro responses of fresh and old calli of japonica variety, Taipei 309, and indica variety, Qiuguiai 11, were investigated with different culture procedures to develop an efficient protocol to improve the regeneration efficiency of long-term cultured calli of rice. Pre-culture media were used to restore the declined plant regeneration ability in long-term cultured calli. Both proliferation rates and regeneration efficiencies were compared between 2 varieties with fresh and old calli before and after using pre-culture media. It was clear that in-vitro culture responses were being declined after the second subculture of calli, indicating the loss of abilities to regenerate plants from long-term cultured calli in rice. An efficient protocol developed in this study with a pre-culture medium containing 10 mg/l BA,4 mg/l ABA, 1 mg/l NAA and 3% maltose showed 7-9 folds increase of in-vitro responses in longterm cultured old calli. This protocol not only restored the declined in-vitro ability, but also reduced the somaclonal variation, especially in indica variety, Qiuguiai 11. Furthermore, japonica variety, Taipei 309, performed well in the proliferation cultures before and after using pre-culture media, showing higher proliferation rates with both fresh and old calli, comparing with indica variety, Qiuguiai 11. Higher regeneration efficiencies with less number of albino plants were given by indica variety, Qiuguiai 11 throughout the study. This study clearly indicates that the invitro culture responses in rice depend mainly on the varieties of different subspecies, culture conditions and compositions, and the stages of the culture procedures.

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“…Os bons resultados obtidos com N 6 com AIA e GA 3 (100% de ápices caulinares não necrosados)(TABELA 2), concordam com Morel et al (1968), citado por Bhojwani & Razdan (1983) que reportaram o favorecimento do crescimento e diferenciação dos propágulos de dália na presença de pequenas concentrações de GA 3 . Esses autores verificaram também que o GA 3, em combinações com BAP e ANA, foi essencial para a regeneração de plântulas de mandioca a partir de ápices caulinares.…”

Section: Resultados E Discussão Experimento I -Meios Nutritivosunclassified

Cultivo in vitro de ápices caulinares de abacaxizeiro para limpeza clonal em relação à fusariose

Albuquerque

Câmara

Menezes

et al. 2000

Sci. agric. (Piracicaba, Braz.)

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RESUMO: O presente trabalho teve como objetivo adequar um meio nutritivo ao cultivo de ápices caulinares de abacaxizeiro, cv. Pérola, visando a limpeza clonal de plantas infectadas por Fusarium subglutinans, agente causal da fusariose. Inicialmente, ápices de mudas sadias, tipo coroa, foram retirados sob condições assépticas e inoculados em 9 composições de meios de cultivo, com presença e ausência de BAP, GA 3 e AIA. Aos 30, 45 e 60 dias após a inoculação, observou-se o grau de necrose dos explantes. Nos meios selecionados inocularamse ápices caulinares retirados de mudas contaminadas por F. subglutinans, a fim de verificar a viabilidade da limpeza clonal. Os resultados mostraram que houve influência do meio de cultivo na regeneração de plantas e a viabilidade da limpeza clonal de mudas infectadas, também foi constatada uma vez que 100% das plantas regeneradas estavam livres do patógeno. Palavras-chave: Ananas comosus, abacaxi, meio nutritivo, ápice caulinar SHOOT TIP IN VITRO CULTURE OF PINEAPPLE FOR FUSARIUM ELIMINATIONABSTRACT: The object of this study was to find a nutricional means for the cultivation of the shoot tips of Perola pineapples, in order to free them of infections caused by Fusarium subglutinas. The first step was to remove healthy shoot tips from healthy crowns under aseptic conditions and inoculate nine different cultivation media with or whithout traces of BAP, GA3 and AIA. The levels of necrosis in the plants were studied after 30,45 and 60 days. Selected shoot tips removed from the plants contaminated with F. subglutinans were inoculated to check their viability as pathogen-free clones. The results revealed the influence of the cultivation media in the regeneration of plants and the viability of clonal cleansing of infected plants. It was also noted that 100% of the regenerated plants remained pathogen-free.

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Plant tissue culture: Theory and Practice, a Revised Edition

Cited by 271 publications

References 0 publications

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Comparative studies on in-vitro response of fresh and old calli of rice (Oryza sativa L.)

Cultivo in vitro de ápices caulinares de abacaxizeiro para limpeza clonal em relação à fusariose

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