Plant Transformation Strategies

Márquez-Escobar, Verónica Araceli; González‐Ortega, Omar; Rosales‐Mendoza, Sergio

doi:10.1007/978-3-319-90137-4_2

Cited by 2 publications

(1 citation statement)

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“…Furthermore, gene stacking based on site-specific recombination and nuclease activity has a potential for in planta stacking of a large number of genes at a single genomic site of the same plant [ 147 ]. Also, multiple genes could be stacked using plastid transformation with operon-like and polyprotein expression systems [ 148 – 150 ]. CRISPR/Cas9-mediated targeted T-DNA integration and precise knock-in [ 102 , 151 , 152 ] can potentially be used for in planta stacking.…”

Section: Technical Approaches For Plant Biosystems Designmentioning

confidence: 99%

Plant Biosystems Design Research Roadmap 1.0

et al. 2020

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Human life intimately depends on plants for food, biomaterials, health, energy, and a sustainable environment. Various plants have been genetically improved mostly through breeding, along with limited modification via genetic engineering, yet they are still not able to meet the ever-increasing needs, in terms of both quantity and quality, resulting from the rapid increase in world population and expected standards of living. A step change that may address these challenges would be to expand the potential of plants using biosystems design approaches. This represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological systems. Plant biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant genomes. From this perspective, we present a comprehensive roadmap of plant biosystems design covering theories, principles, and technical methods, along with potential applications in basic and applied plant biology research. We highlight current challenges, future opportunities, and research priorities, along with a framework for international collaboration, towards rapid advancement of this emerging interdisciplinary area of research. Finally, we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception, trust, and acceptance.

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