Plant-Virus Interaction on Orchids Infected Odontoglossum ringspot virus (ORSV) in Bogor Botanical Garden, Indonesia

Mahfut, Mahfut; Daryono, Budi Setiadi; Indrianto, Ari; Somowiyarjo, Susamto

doi:10.4108/eai.2-5-2019.2284701

Cited by 6 publications

(9 citation statements)

References 7 publications

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“…ORSV has quite extensive distribution areas in Java, Ujung Pandang, Kalimantan, Bali, and Papua [4]; [8]; [9]; [11]; [12]; [13]; [16]. In general, ORSV infection results in chloroplast damage [10]; [14].…”

Section: Introductionmentioning

confidence: 99%

Identification and Efforts to Control Infection Odontoglossum ringspot virus (ORSV) on Orchid

Mahfut

2021

Int. J. Eng. Scie. and Inform. Technology.

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Odontoglossum ringspot virus (ORSV) is a orchid virus which infection most widely reported and has spread widely in the world, including in Indonesia. Viral infections can degrade the quality of the orchid plant so it is economically detrimental. To study a disease infection, identification is carried out first to determine the most appropriate method of control. These identification techniques include variations in infection symptoms, host range testing, testing of viral titers, observation of particle morphology, genome detection, and analysis of DNA and amino acid sequences. Efforts to control disease can be done through preventive and eradicative actions chemically, biologically, and based on artificial intelligence.

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Section: Introductionmentioning

confidence: 99%

Identification and Efforts to Control Infection Odontoglossum ringspot virus (ORSV) on Orchid

Mahfut

2021

Int. J. Eng. Scie. and Inform. Technology.

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“…Infections by bacteria, fungi, and viruses are still major obstacles in conducting cultivation and development of native orchids in Indonesia (Kumalawati et al, 2011;Mahfut and Daryono, 2014;Mahfut et al, 2016;Daryono et al, 2016;Mahfut et al, 2017;Mahfut et al, 2019b). Cultivating and protecting Phalaenopsis sp.…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny of orchids mycorrhiza isolated from native tropical orchids in Indonesia

Mahfut¹,

Daryono²,

Indrianto³

et al. 2020

MJM

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Aims: Mycorrhiza has an important role as a biocontrol agent. Its association with Phalaenopsis amabilis was molecularly identified through rDNA-ITS sequence analysis. The aims of the study were to identify molecular of orchids mycorrhiza isolate from native tropical orchids in Indonesia, conducted as one of native orchid conservation efforts in Indonesia. Methodology and results: One group of Ceratobasidium were isolated from the root of orchid plant in Yogyakarta based on morphological and microscopical analysis. The results of molecular analysis showed 600-750 bp of DNA products located on the ITS1-5.8S-ITS4 region. The sequenced products showed insertion and substitution occurances, which may result in strain diversity and possible variation. Reconstruction of phylogenetic trees using Maximum Parsimony and Bootstrap-1000 approach showed showed the Indonesian isolate is at the basal clade and already far apart from the other isolates. Conclusion, significance and impact of study: Isolate Ceratobasidium from Yogyakarta, Indonesia successfully isolated based on identification of rDNA-ITS sequences. Results of this study were expected to become the basic information in an effort of native orchid cultivation and protection against infectious diseases in Indonesia. The study was the first to report regarding Ceratobasidium isolated from native tropical orchids in Indonesia.

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“…Hasil uji serologis membuktikan bahwa terdapat infeksi campuran, yaitu Odontoglossum ringspot virus (ORSV) dan Cymbidium mosaic virus (CymMV). Keduanya termasuk virus penting karena dilaporkan memiliki persebaran penyakit yang luas di dunia termasuk sudah sampai di Indonesia (Mahfut & Setiadi Daryono, 2014;Mahfut & Daryono, 2019;Mahfut, 2019;Mahfut et al, 2019aMahfut et al, , 2019bMahfut, 2020aMahfut, , 2020b Upaya perlindungan terhadap infeksi penyakit harus dilakukan agar keberadaaan anggrek alam di Kebun Raya Liwa terjaga kelestariannya. Berdasarkan informasi langsung dari Kepala PLT Kebun Raya Liwa, Bapak Sukimin, S.Ip.,M.M.…”

Section: Pendahuluanunclassified

Penerapan Teknologi Kesehatan Tepat Guna di Era Industri 4.0 dalam Perlindungan Anggrek Alam Terhadap Infeksi Penyakit di Kebun Raya

Mahfut

Heningtyas

Sukimin³

2021

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Keberadaan anggrek alam di Kebun Raya Liwa merupakan salah satu kekayaan flora asli Sumatera Bagian Selatan yang perlu dijaga kelestariannya. Berdasarkan hasil identifikasi diketahui bahwa beberapa individu anggrek menunjukkan gejala terinfeksi virus. Tujuan dari kegiatan pengabdian ini adalah untuk mencari metode pengendalian penyakit virus yang tepat, efektif, dan efisien. Metode yang dilakukan melalui pendekatan beberapa bidang ilmu seperti biologi, proteksi tanaman, dan ilmu komputer. Aplikasi induksi ketahanan agensia hayati mikoriza endofit sebagai agen biokontrol yang mampu menekan replikasi penyakit pada tanaman dapat digunakan sebagai cara yang murah dan ramah lingkungan dibandingkan pestisida. Selain itu, penggunaan metode sistem pakar diagnosis penyakit tanaman dengan metode case-base reasoning berbasis web. Pelaksanaan kegiatan pengabdian ini masih dalam masa pandemi Covid-19 sehingga sebagian besar kegiatan dilakukan secara daring (online). Keseluruhan metode identifikasi penyakit berbasis ilmu biologi, penyakit tanaman, dan ilmu komputer serta upaya pengendaliannya yang merupakan hasil produk (output) kegiatan pengabdian masyarakat ini diadopsi oleh pihak Kebun Raya Liwa sebagai upaya pengembangan dan pelestarian koleksi anggrek alam di era industri 4.0. Hasil evaluasi diperoleh peningkatan pengetahuan dan pemahaman peserta pelatihan dengan peningkatan sebesar 21,64 point (34,72%). Hasil kegiatan ini diharapkan tidak hanya sebatas peningkatan pengetahuan dan pemahaman dalam peserta kegiatan, tetapi juga dapat menerapkan dan menularkan pengetahuan yang diperoleh selama pelatihan kepada komunitas pecinta anggrek dan masyarakat luas.

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Plant-Virus Interaction on Orchids Infected Odontoglossum ringspot virus (ORSV) in Bogor Botanical Garden, Indonesia

Cited by 6 publications

References 7 publications

Identification and Efforts to Control Infection Odontoglossum ringspot virus (ORSV) on Orchid

Identification and Efforts to Control Infection Odontoglossum ringspot virus (ORSV) on Orchid

Molecular phylogeny of orchids mycorrhiza isolated from native tropical orchids in Indonesia

Penerapan Teknologi Kesehatan Tepat Guna di Era Industri 4.0 dalam Perlindungan Anggrek Alam Terhadap Infeksi Penyakit di Kebun Raya

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