Plantago australis Hydroethanolic Extract-Loaded Formulations: Promising Dressings for Wound Healing

Reinhardt, Luiza Steffens; Henn, Jeferson Gustavo; Morás, Ana Moira; Sperotto, Nathalia Denise de Moura; Ferro, Matheus Bernardes; Cao, Zhi; Roehe, Adriana Vial; Petry, Adriana Ubirajara Silva; Nugent, Michael; Moura, Dinara Jaqueline

doi:10.1007/s43450-021-00126-9

Cited by 6 publications

(7 citation statements)

References 36 publications

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“…Magnetic-based models allow us to effectively produce homogeneous 3D aggregates and with desirable compactness [ 1 , 17 ]. Of the twenty-five studies included in this review, nine studies used only magnetic levitation [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ], eleven studies used only the bioprinting method [ 14 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ], and three studies used both methods [ 15 , 36 , 37 ]. Only two studies used the magnetic ring formation method [ 22 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…The 24-well plates were the most used for studies using the levitation method; whereas, the 96-well plates were the most used for the bioprinting method. More precisely, nine studies used the 24-well Bio-Assembler Kit [ 15 , 23 , 24 , 25 , 26 , 30 , 32 , 37 , 39 ], eight studies used the 96-well Bioprinting Kit [ 14 , 27 , 28 , 29 , 31 , 33 , 35 , 36 ], two studies used both 6-well and 24-well Bio-Assembler Kit [ 17 , 19 ], one study used only the 6-well Bio-Assembler Kit [ 34 ], and one study used the 96-well Ring Drive for ring structure formation [ 38 ]. There is an additional study that used tissue culture Petri dishes covered by a top cover with an attached neodymium magnet [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…Human fetal osteoblast cells (hFOB 1.19) were used for bioprinting m3D homotypic spheroids to investigate normal bone physiology and bone tissue engineering and regeneration [ 14 ]. Glioblastoma cells (U87) were used for bioprinting m3D spheroids to mimic the wound healing process, with the purpose of testing new wound dressings containing the Plantago australis hydroethanolic extract [ 33 ]. In addition, m3D homotypic cultures of human telomerase reverse transcriptase (hTert)-immortalized retinal pigment epithelium (RPE) cells, squamous cell carcinoma lines (HPV negative or positive), and osteosarcoma cells (FUCCI cell line) were produced to study the hypoxia-dependent radioprotective phenotype; however, RPE cells failed in forming spheroids [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…One is thermoresponsive and made of Poly(urethane acrylate)-poly(glycidyl methacrylate to provide regulation of the cellular alignment and cell sheet transfer [ 21 ]. The other is made of polyvinyl alcohol (PVA) solutions, combined with semi-solid Plantago australis hydroethanolic extract (PAHE), to produce wound dressings and provide a continuous drug release source [ 33 ]. A m3D culture has also been produced by magnetic levitation in the presence of a natural hydrogel composed of M13-derived bacteriophage particles, displaying a ligand peptide (termed RGD-4C) that targets αv integrins, magnetic iron oxide (Fe 3 O 4 , magnetite), and gold nanoparticles [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…Souza and colleagues [ 22 ] developed glioblastoma spheroids and the authors observed not only morphological similarities, but also a molecular similarity to orthotopic human tumor xenografts from immunodeficient mice. Reinhardt et al also produced glioblastoma m3D structures to develop wound dressings and maximize the healing process [ 33 ]. Menegakis and his team developed m3D cultures using retinal and skin squamous cell carcinoma lines to study the quiescence induction under hypoxia and its HPV-driven prevention.…”

Section: Resultsmentioning

confidence: 99%

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Magnetic-Based Human Tissue 3D Cell Culture: A Systematic Review

Marques

Fernandes

Tavares

et al. 2022

IJMS

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Cell-based assays, conducted on monolayer (2D) cultured cells, are an unquestionably valuable tool for biomedical research. However, three-dimensional (3D) cell culture models have gained relevance over the last few years due to the advantages of better mimicking the microenvironment and tissue microarchitecture in vivo. Recent magnetic-based 3D (m3D) cell culture systems can be used for this purpose. These systems are based on exposing magnetized cells to magnetic fields by levitation, bioprinting, or ring formation to promote cell aggregation into 3D structures. However, the successful development of these structures is dependent on several methodological characteristics and can be applied to mimic different human tissues. Thus, a systematic review was performed using Medline (via Pubmed), Scopus, and Web of Science (until February 2022) databases to aggregate studies using m3D culture in which human tissues were mimicked. The search generated 3784 records, of which 25 met the inclusion criteria. The usability of these m3D systems for the development of homotypic or heterotypic spheroids with or without scaffolds was explored in these studies. We also explore methodological differences specifically related to the magnetic method. Generally, the development of m3D cultures has been increasing, with bioprinting and levitation systems being the most used to generate homotypic or heterotypic cultures, mainly to mimic the physiology of human tissues, but also to perform therapeutic screening. This systematic review showed that there are areas of research where the application of this method remains barely explored, such as cancer research.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Magnetic-Based Human Tissue 3D Cell Culture: A Systematic Review

Marques

Fernandes

Tavares

et al. 2022

IJMS

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Bioprinted research models of urological malignancy

Wang,

Mao,

Wang

et al. 2024

Exploration

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Urological malignancy (UM) is among the leading threats to health care worldwide. Recent years have seen much investment in fundamental UM research, including mechanistic investigation, early diagnosis, immunotherapy, and nanomedicine. However, the results are not fully satisfactory. Bioprinted research models (BRMs) with programmed spatial structures and functions can serve as powerful research tools and are likely to disrupt traditional UM research paradigms. Herein, a comprehensive review of BRMs of UM is presented. It begins with a brief introduction and comparison of existing UM research models, emphasizing the advantages of BRMs, such as modeling real tissues and organs. Six kinds of mainstream bioprinting techniques used to fabricate such BRMs are summarized with examples. Thereafter, research advances in the applications of UM BRMs, such as culturing tumor spheroids and organoids, modeling cancer metastasis, mimicking the tumor microenvironment, constructing organ chips for drug screening, and isolating circulating tumor cells, are comprehensively discussed. At the end of this review, current challenges and future development directions of BRMs and UM are highlighted from the perspective of interdisciplinary science.

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