Plants as Sources of Airborne Bacteria, Including Ice Nucleation-Active Bacteria

Lindemann, Julianne; Constantinidou, Helen-Isis A.; Barchet, W.R.; Upper, Christen D.

doi:10.1128/aem.44.5.1059-1063.1982

Cited by 251 publications

(149 citation statements)

References 29 publications

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“…Bacteria.-Our findings agree with previous studies of aerosolized microbes that have reported dominance of Actinobacteria, Cyanobacteria, Firmicutes, Bacteroidetes, and Proteobacteria, particularly Alphaproteobateria and Gammaproteobacteria (Lindemann et al 1982, Bowers et al 2013, Striluk et al 2016. These findings are also in general agreement with the limited number of studies that have examined the microbes in clouds and precipitation (Va€ ıtilingom et al 2012, Santl-Temkiv et al 2015, Amato et al 2017a.…”

Section: Taxonomic Composition Of Sequence Librariessupporting

confidence: 92%

Spatiotemporal patterns of microbial composition and diversity in precipitation

Aho

Weber

Christner

et al. 2019

Ecological Monographs

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Microbes in the atmosphere have broad ecological impacts, including the potential to trigger precipitation through species and strains that act as ice nucleation particles. To characterize spatiotemporal trends of microbial assemblages in precipitation we sequenced 16S (bacterial) and 18S (fungal) rRNA gene amplicon libraries collected from 72 precipitation events in three U.S. states (Idaho, Louisiana, and Virginia) over four seasons. We considered these data from the perspective of a novel metacommunity framework. In agreement with our heuristic, we found evidence for distinct mechanisms underlying the composition and diversity of bacterial and fungal assemblages in precipitation. Specifically, we determined that (1) bacterial operational taxonomic unit (OTU) composition of precipitation was strongly associated with macroscale drivers including season and high-altitude characteristics of storms; (2) fungal OTU composition was strongly correlated with mesoscale drivers including particular spatial locations; (3) b-diversity (heterogeneity of taxa among samples) for both bacteria and fungi was largely maintained by turnover of taxa; however, (4) bacterial assemblages had higher contributions to total b-diversity from nestedness (i.e., lower richness assemblages were largely taxonomic subsets of richer assemblages), due to losses of taxa during dispersal, particularly among potential ice nucleation active bacteria; and (5) fungal assemblages had higher contributions to total b-diversity from turnover due to OTU replacement. Spatiotemporal trends in precipitation-borne metacommunities allowed delineation of a large number of statistically significant indicator taxa for particular sites and seasons, including trends for bacteria that are potentially ice nucleation active. Our findings advance understanding regarding the dispersion of aerosolized microbes via wet deposition, and the development of theory concerning potential assembly rules for bioaerosol assemblages.

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Section: Taxonomic Composition Of Sequence Librariessupporting

confidence: 92%

Spatiotemporal patterns of microbial composition and diversity in precipitation

Aho

Weber

Christner

et al. 2019

Ecological Monographs

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“…Across pathovars, it infects a wide variety of species including tomato, kiwifruit, and soybean (Sarkar and Guttman 2004). After arriving on a suitable host by wind or rain-mediated dispersal, it begins as an epiphyte on leaf surfaces (Lindemann et al 1982;Constantinidou et al 1990). Once reaching a sufficiently high density as determined via quorum sensing, the bacterium enters the apoplast (internal intercellular space) of the leaf through stomata or wounds (Quiñones et al 2005;Misas-Villamil et al 2013).…”

Section: Study Systemmentioning

confidence: 99%

Phage resistance evolution in vitro is not reflective of in vivo outcome in a plant‐bacteria‐phage system*

Hernandez

Koskella

2019

Evolution

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The evolution of resistance to parasites is fundamentally important to disease ecology, yet we remain unable to predict when and how resistance will evolve. This is largely due to the context‐dependent nature of host‐parasite interactions, as the benefit of resistance will depend on the abiotic and biotic environment. Through experimental evolution of the plant pathogenic bacterium Pseudomonas syringae and two lytic bacteriophages across two different environments (high‐nutrient media and the tomato leaf apoplast), we demonstrate that de novo evolution of resistance is negligible in planta despite high levels of resistance evolution in vitro. We find no evidence supporting the evolution of phage‐selected resistance in planta despite multiple passaging experiments, multiple assays for resistance, and high multiplicities of infection. Additionally, we find that phage‐resistant mutants (evolved in vitro) did not realize a fitness benefit over phage‐sensitive cells when grown in planta in the presence of phage, despite reduced growth of sensitive cells, evidence of phage replication in planta, and a large fitness benefit in the presence of phage observed in vitro. Thus, this context‐dependent benefit of phage resistance led to different evolutionary outcomes across environments. These results underscore the importance of studying the evolution of parasite resistance in ecologically relevant environments.

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“…However, there has not been a formal demonstration of this phenomenon, mostly due to the paucity of studies of microbial flux in the atmosphere (Morris et al, 2013a). Nevertheless, in one of the few studies of microbial flux, upward flux over crops was the highest over the crops having the most dense microbial populations on their leaves (Lindemann et al, 1982). Overall, plant quality affects the abundance of biological ice nuclei in the atmosphere, with the greatest concentrations being observed over agricultural land than over suburban land or forests (Bowers et al, 2011).…”

Section: Componentmentioning

confidence: 99%

“…Emission rates and average atmospheric concentrations for fungal spores are estimated to be on the same order of magnitude (Elbert et al, 2007;Sesartic & Dallafior, 2011;Despres et al, 2012;Huffman et al, 2012;Santl-Temkiv et al, 2013). Vegetation is implicated as the main source of air-borne microorganisms, and more so than bare soil (Lindemann et al, 1982;Lighthart, 1997;Burrows et al, 2009). In a study of the composition of hailstones, the culturable bacterial community was skewed toward groups of bacteria most common on plants, whereas their dissolved organic matter content was mainly from soils (vs. plants) ( Santl-Temkiv et al, 2013).…”

Section: Landscapes Aerosols and Phenomena Coherent With Bioprecipitmentioning

confidence: 99%

“…(Upper & Hirano, 1991;Jones & Harrison, 2004). Bacterial launch, in particular, is most efficient during rain, when droplets shake leaves and release poorly attached particles (Upper & Hirano, 1991), or at midday, when leaves are dry and solar heating promotes convection (Lindemann et al, 1982). Evidence exists for meteorological correlation between bacterial concentrations and atmospheric conditions (e.g.…”

Section: Landscapes Aerosols and Phenomena Coherent With Bioprecipitmentioning

confidence: 99%

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Bioprecipitation: a feedback cycle linking Earth history, ecosystem dynamics and land use through biological ice nucleators in the atmosphere

Morris

Conen

Huffman

et al. 2013

Global Change Biology

259

225

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Landscapes influence precipitation via the water vapor and energy fluxes they generate. Biologically active landscapes also generate aerosols containing microorganisms, some being capable of catalyzing ice formation and crystal growth in clouds at temperatures near 0 °C. The resulting precipitation is beneficial for the growth of plants and microorganisms. Mounting evidence from observations and numerical simulations support the plausibility of a bioprecipitation feedback cycle involving vegetated landscapes and the microorganisms they host. Furthermore, the evolutionary history of ice nucleation-active bacteria such as Pseudomonas syringae supports that they have been part of this process on geological time scales since the emergence of land plants. Elucidation of bioprecipitation feedbacks involving landscapes and their microflora could contribute to appraising the impact that modified landscapes have on regional weather and biodiversity, and to avoiding inadvertent, negative consequences of landscape management.

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Plants as Sources of Airborne Bacteria, Including Ice Nucleation-Active Bacteria

Cited by 251 publications

References 29 publications

Spatiotemporal patterns of microbial composition and diversity in precipitation

Spatiotemporal patterns of microbial composition and diversity in precipitation

Phage resistance evolution in vitro is not reflective of in vivo outcome in a plant‐bacteria‐phage system*

Bioprecipitation: a feedback cycle linking Earth history, ecosystem dynamics and land use through biological ice nucleators in the atmosphere

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