Plaque assay and primary isolation of influenza a viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin

Tobita, Kiyotake; Sugiura, Akira; Enomoto, C.; Furuyama, Masako

doi:10.1007/bf02123572

Cited by 369 publications

(224 citation statements)

References 10 publications

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“…Nasal lavage samples from each day were frozen at Ϫ80°C and later cultured for rhinovirus or influenza virus using standard techniques (Gwaltney et al, 1989;Tobita, Sugiura, Enomoto, & Furuyama, 1975).…”

Section: Viral Cultures and Antibody Responsementioning

confidence: 99%

Objective and subjective socioeconomic status and susceptibility to the common cold.

Cohen¹,

Alper²,

Doyle³

et al. 2008

Health Psychology

268

217

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Objective:We ask whether subjective socioeconomic status (SES) predicts who develops a common cold when exposed to a cold virus. Design: 193 healthy men and women ages 21-55 years were assessed for subjective (perceived rank) and objective SES, cognitive, affective and social dispositions, and health practices. Subsequently, they were exposed by nasal drops to a rhinovirus or influenza virus and monitored in quarantine for objective signs of illness and self-reported symptoms. Main Outcome Measures: Infection, signs and symptoms of the common cold, and clinical illness (infection and significant objective signs of illness). Results: Increased subjective SES was associated with decreased risk for developing a cold for both viruses. This association was independent of objective SES and of cognitive, affective and social disposition that might provide alternative spurious (third factor) explanations for the association. Poorer sleep among those with lesser subjective SES may partly mediate the association between subjective SES and colds. Conclusions: Increased Subjective SES is associated with less susceptibility to upper respiratory infection, and this association is independent of objective SES, suggesting the importance of perceived relative rank to health.

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Section: Viral Cultures and Antibody Responsementioning

confidence: 99%

Objective and subjective socioeconomic status and susceptibility to the common cold.

Cohen¹,

Alper²,

Doyle³

et al. 2008

Health Psychology

268

217

View full text Add to dashboard Cite

show abstract

“…Culture supernatants were harvested and stored at Ϫ80°C. The virus titer was determined by a plaque assay, as previously described (16). To determine the virus titer in the lung, the mice were sacrificed under light diethyl ether anesthesia.…”

Section: Influenza Virusmentioning

confidence: 99%

Baculovirus Induces an Innate Immune Response and Confers Protection from Lethal Influenza Virus Infection in Mice

Abe¹,

Takahashi

Hamazaki³

et al. 2003

The Journal of Immunology

221

173

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A recombinant baculovirus expressing the hemagglutinin gene of the influenza virus, A/PR/8/34 (H1N1), under the control of the chicken β-actin promoter, was constructed. To determine the induction of protective immunity in vivo, mice were inoculated with the recombinant baculovirus by intramuscular, intradermal, i.p., and intranasal routes and then were challenged with a lethal dose of the influenza virus. Intramuscular or i.p. immunization with the recombinant baculovirus elicited higher titers of antihemagglutinin Ab than intradermal or intranasal administration. However, protection from a lethal challenge of the influenza virus was only achieved by intranasal immunization of the recombinant baculovirus. Surprisingly, sufficient protection from the lethal influenza challenge was also observed in mice inoculated intranasally with a wild-type baculovirus, as evaluated by reductions in the virus titer, inflammatory cytokine production, and pulmonary consolidations. These results indicate that intranasal inoculation with a wild-type baculovirus induces a strong innate immune response, which protects mice from a lethal challenge of influenza virus.

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“…Virus yield was quantified by the stanGene Therapy dard plaque-forming assay of virus suspensions in MDCK cells. 55 …”

Section: H1n1 Titer In the Lungmentioning

confidence: 99%

Adenovirus-mediated transfer of heme oxygenase-1 cDNA attenuates severe lung injury induced by the influenza virus in mice

et al. 2001

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Plaque assay and primary isolation of influenza a viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin

Cited by 369 publications

References 10 publications

Objective and subjective socioeconomic status and susceptibility to the common cold.

Objective and subjective socioeconomic status and susceptibility to the common cold.

Baculovirus Induces an Innate Immune Response and Confers Protection from Lethal Influenza Virus Infection in Mice

Adenovirus-mediated transfer of heme oxygenase-1 cDNA attenuates severe lung injury induced by the influenza virus in mice

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