Plasma cell-free DNA as an indicator of severity of injury in burn patients

Chiu, Tor; Young, Richard B.; Chan, Lisa; Burd, Andrew; Lo, Dennis

doi:10.1515/cclm.2006.003

Cited by 66 publications

(53 citation statements)

References 30 publications

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“…For group S, total body surface area (TBSA) burned was 37.8 ± 2.1% (range 25-64%) and for group N-S it was 54.4 ± 9.1% (26-96%; n.s.). At admission, ABSI score was 8.8 ± 0.3 points (range 6-12) in group S and 11.7 ± 0.6 points (range [10][11][12][13][14][15] in group N-S (p \ 0.01).…”

Section: Resultsmentioning

confidence: 93%

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Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

Altrichter

Zedler

Kraft

et al. 2010

Eur J Trauma Emerg Surg

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The predictive value of circulating free DNA/neutrophil extracellular traps (cf-DNA/NETs) has recently been shown in patients with major trauma for sepsis, multiple organ failure, and mortality. Here we report on the predictive potential of cf-DNA/NETs for mortality in patients with severe burn injury. In a prospective study 32 patients with severe burn injury were included. Blood samples were sequentially obtained on day 1, 3, 5, and 7 after admission. cf-DNA/NETs was directly quantified from plasma by means of rapid fluorescence assay. Time kinetics of cf-DNA/NETs were correlated with clinical data, C-reactive protein (CRP), procalcitonin (PCT), and interleukin (IL)-6. Furthermore sensitivity, specificity, and positive and negative predictive value, as well as receiver operation characteristic (ROC) curves were calculated. Seven patients died within the first month after burn injury. cf-DNA/NETs values from these patients were significantly increased already on day 1 and 3 after admission compared with patients who survived (p < 0.01). In contrast, PCT levels of nonsurvivors were significantly elevated on day 3 and 5 (p < 0.01), while CRP and IL-6 did not show any significant difference between survivors and nonsurvivors. At a cutoff of 255 ng/ml, cf-DNA/NETs had sensitivity of 0.8 and specificity of 0.74. ROC revealed largest areas under the curve (AUC) for cf-DNA/NETs on day 1 (0.851) and 3 (0.883) after admission. For all values between day 1 and 7, AUC was 0.815. cf-DNA/NETs seems to be a rapid, valuable marker for prediction of mortality in burn patients. A larger confirmation trial ought to be carried out.

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Section: Resultsmentioning

confidence: 93%

“…Recently, plasma circulating free DNA (cf-DNA) has been suggested as an indicator of severity of injury in burn patients [12,13]. However, in these studies, time-consuming real-time quantitative polymerase chain reaction (PCR) was used to detect cf-DNA, and this parameter was thought to solely reflect the severity of tissue damage.…”

Section: Introductionmentioning

confidence: 99%

Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

Altrichter

Zedler

Kraft

et al. 2010

Eur J Trauma Emerg Surg

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“…Conditions such as cardiovascular disease [3], sepsis [4] and cancer [5] have been associated with elevated cf-DNA concentrations. The degree or severity of injury associated with traumatic events including but not limited to blunt trauma and burn victims, have also been shown to be reflected in cf-DNA concentrations [6,7].…”

Section: Tine Component Of a Long-term Athlete Monitoring Program (Ltmentioning

confidence: 99%

Cell free DNA as a marker of training status in weightlifters

Gentles¹,

Hornsby²,

Coniglio³

et al. 2017

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ABSTRACT:The purpose of this investigation was to elucidate the changes in cf-DNA as it relates to fluctuations in resistance training workloads and intensities. The relationship between cell free DNA (cf-DNA), C-reactive protein (CRP), creatine kinase (CK), testosterone (T), cortisol (C), testosterone-cortisol ratio (T:C), body mass and body composition were also examined. Eight weightlifters (5 males and 3 females, age = 25 ± 3.5 yr, body mass = 88.3 ± 22.7 kg, height = 173.8 ±8.4 cm) volunteered to participate in this study. Venous blood samples, body mass and body composition were taken six times, each corresponding to the end of a training phase. CK (p = 0.018, η² = 0.409) and CK %∆ (p < 0.001, η² = 0.594) were the only biochemical variables to reach statistical significance at any point. A number of statistically significant correlations were found among variables. VLD4wk was related to CK %∆ (r = 0.86), VLD4wk %∆ was related CK %∆ (r = 0.86) and TID1wk was related to CRP (r = 0.83). cf-DNA %∆ was correlated with CRP and CRP %∆ (r = 0.83 and 0.86, respectively). CRP and CRP %∆ were correlated with BF % (r = 0.94 and 0.92, respectively). CK and CK %∆ were both related to T:C (r = 0.94 and 0.89, respectively) and T:C %∆ (r = 0.87 and 0.86, respectively). The correlation between cf-DNA and CRP suggests that cf-DNA may be a valuable indicator of inflammation in weightlifters. CITATION:Gentles JA, Hornsby WG, Coniglio CL et al. Cell free DNA as a marker of training status in weightlifters.

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“…-after acute events such as myocardial or cerebral stroke, burns or major trauma ( [209][210][211][212][213][214][215][216][217]; chapter "CNAPS and General Medicine") -the early detection of bacterial sepsis and the monitoring sepsis treatment [218][219][220] -viral infections in the acute and chronic stage [221,222] -the monitoring of autoimmune diseases such as systemic lupus erythematosus [223][224][225][226] -metabolic disease such as diabetes mellitus (reviewed in [227]; chapter "Circulating Nucleic Acids and Diabetes Mellitus") -graft-versus-host disease after transplantation [228] -the early detection of preeclampsia [229,230] -the detection of cffDNA in maternal plasma during pregnancy ( [231,232];…”

Section: Other Areas Of Disease Monitoring By Cnapsmentioning

confidence: 99%

CNAPS in Therapy Monitoring

Holdenrieder

2014

Advances in Predictive, Preventive and Personalised Medicine

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Monitoring of disease state and of therapy response is highly relevant for efficient patient management. Monitoring tools comprise observation of clinical signs and performing specific examinations such as imaging or blood analyses. This review focusses on the relevance of blood-based biomarker monitoring by circulating nucleic acids for diverse indications that is exemplified on patients who develop or suffer from cancer disease. These indications include (i) screening of patient groups who have a risk to develop a disease, (ii) monitoring response to local or systemic therapies in patients with a defined diagnosis and (iii) early detection of disease recurrence after the primary therapy has ended. Useful biomarkers have to fulfill the highest methodical, pre-analytical and clinical quality criteria and have to be implemented in standardized patient management procedures. The current situation of circulating nucleic acids is summarized on the levels of genetic, epigenetic, transcript, non-coding RNA and nucleosome markers and an outlook is presented as to how these markers can be integrated into a future strategy that enables a personalized management of the patients.

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Plasma cell-free DNA as an indicator of severity of injury in burn patients

Cited by 66 publications

References 30 publications

Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

Cell free DNA as a marker of training status in weightlifters

CNAPS in Therapy Monitoring

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