Plasma concentration gradient of steroid hormones across human mammary tumours in vivo

Landeghem, A.A.J. van; Poortman, J.; Deshpande, N.; Martino, L. Di; Tarquini, A; Thussen, J.H.H.; Schwärz, F.

doi:10.1016/0022-4731(81)90010-8

Cited by 65 publications

(16 citation statements)

References 14 publications

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“…For E2 estimations, the ultrasensitive system was used. The results of these assays were compared with the results obtained with other commercial immunoassays, which in turn had been validated by comparison with in-house immunoassays, making use of steroid extraction and purification by column chromatography (testosterone (16), E2 (17), androstenedione (18), E1 (19) and DHEAS (20)). The same procedure was used for SHBG, where the in-house method used ammonium sulphate precipitation (21).…”

Section: Hormone Measurementsmentioning

confidence: 99%

A direct approach to the estimation of the origin of oestrogens and androgens in elderly men by comparison with hormone levels in postmenopausal women

et al. 2005

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Objective: The origin of oestrogens in men is only partly understood. From infusion studies with radioactively labelled hormones, we know that oestradiol (E2) and oestrone (E1) are either directly secreted by the testes and adrenal glands or peripherally produced from testicular or adrenal androgens. Design and methods: We determined E2, E1, androstenedione, testosterone and dehydroepiandrosterone sulphate (DHEAS) in 292 elderly men and 367 postmenopausal women. We considered postmenopausal women as men without testes, assuming that the postmenopausal ovary is not endocrinologically active and that the testes do not contribute to circulating levels of DHEAS. Subjects were stratified by DHEAS levels to adjust for differences in DHEAS levels between sexes. For men and women separately, mean levels of E2, E1, androstenedione and testosterone were calculated per DHEAS stratum. The relative direct and indirect contributions of the testes to steroid levels in men were calculated by the formula [(C m -C f )/C m ] £ 100%, in which C m and C f represent the mean concentrations of the steroid in men and women respectively. Results: The relative contributions (%) of the testes to hormone levels per DHEAS stratum (, 2, 2-4, 4 -6 and .6 mmol/l) respectively were, for E2, 72%, 60%, 52% and 44%; for E1, 54%, 47%, 35% and 34%; for androstenedione, 14%, 4%, 12% and 0%; and, for testosterone, 88%, 88%, 87% and 83%. Conclusions: We conclude that in elderly men dependent on DHEAS levels, 44-72% of E2 and 34-54% of E1 originate directly or indirectly from the testes.European Journal of Endocrinology 152 261-268

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Section: Hormone Measurementsmentioning

confidence: 99%

A direct approach to the estimation of the origin of oestrogens and androgens in elderly men by comparison with hormone levels in postmenopausal women

et al. 2005

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“…The ether was evaporated and the residue dissolved in an assay buffer. The characteristics of the antiserum used are given by VAN LANDEGHEM et al (1981). The immunoassay procedure was similar to the one described for ~:ortisol, except that preincubation with antiserum was not performed.…”

Section: Hormone Analysismentioning

confidence: 99%

A pilot study of the social correlates of levels of urinary cortisol, prolactin, and testosterone in wild long-tailed macaques (Macaca fascicularis)

et al. 1991

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ABSTRACT. Urine samples were collected from individuals in a wild population of Sumatran long-tailed macaques (Macacafascicularis), and the levels of cortisol, immunoreactive prolactin, and (for males) testosterone were determined. The amount of foraging during the 2 hr preceding urine collection were found to affect the levels of urinary cortisol, but not those of the other hormones. Immigration into a new group and having one's infant kidnapped led to increased levels of cortisol. Levels of cortisol and testosterone were correlated both within and between individuals, whereas prolactin varied independently. The effects of age, reproductive status, and social rank on the mean values of individuals were also examined. Lactating females had higher prolactin levels than nonlactating ones; reproductive state interacted with the age effect on prolactin and possibly cortisol. No effects of social status were found in spite of a small, but consistent effect of rank on birth rate in this population. Among males, age and rank are strongly linked. The low ranking old males had increased levels of cortisol, even though the younger high-ranking males were involved in the fiercest conflicts.

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“…Estrone (El) and 17P-estradiol (E2) were determined by radioimmunoassay after plasma extraction with ether and after purification and separation by chromatography on Sephadex LH-20 columns with toluenemethanol 9223 as eluant (Van Landeghem, 1981). Androstenedione (Adione) was measured by radioimmunoassay after plasma extraction with diethylether (Van Landeghem, 1981).…”

Section: Hormonal Assaysmentioning

confidence: 99%

“…Androstenedione (Adione) was measured by radioimmunoassay after plasma extraction with diethylether (Van Landeghem, 1981).…”

Section: Hormonal Assaysmentioning

confidence: 99%

The onset of puberty and the availability of sex steroids in female gymnasts

Ridder¹,

Zonderland²,

Thijssen

et al. 1993

American J Hum Biol

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Plasma levels of gonadotropins, (sex) steroids, SHBG bound fractions of 17β-estradiol (E2) and testosterone (T), and sex-hormone-binding globulin (SHBG) were compared at the onset of puberty in female gymnasts (n = 7) and two groups of schoolgirls with similar anthropometric characteristics. Ten schoolgirls were matched to the gymnasts on the basis of a sum of skinfolds and the waist/hip ratio, while 12 other girls were selected on the basis of the stature and bicristal breadth of the female gymnasts. All girls were in the second stage of breast development (M2) and were classified as being in the first stage (M1) 6 months earlier. When female gymnasts were compared to nonathletic schoolgirls with similar physical characteristics at the same stage of early puberty, the former had significantly lower levels of plasma luteinizing hormone (LH), total and available E2, and T. In conclusion, female gymnasts have significantly lower LH, E2, and T plasma levels than nonathletic schoolgirls in early puberty. The E2 and T plasma levels in early female gymnasts are not related to the individual physical characteristics, i.e., fat mass, short stature, or small bicristal breadth. © 1993 Wiley-Liss, Inc.

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Plasma concentration gradient of steroid hormones across human mammary tumours in vivo

Cited by 65 publications

References 14 publications

A direct approach to the estimation of the origin of oestrogens and androgens in elderly men by comparison with hormone levels in postmenopausal women

A direct approach to the estimation of the origin of oestrogens and androgens in elderly men by comparison with hormone levels in postmenopausal women

A pilot study of the social correlates of levels of urinary cortisol, prolactin, and testosterone in wild long-tailed macaques (Macaca fascicularis)

The onset of puberty and the availability of sex steroids in female gymnasts

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