Plasma gh, igf-i, and conception rate in cattle treated with low doses of recombinant bovine gh

Bilby, C.R.; Bader, J.F.; Salfen, B E; Youngquist, R.S.; Murphy, Clifton N.; Garverick, H.A.; Crooker, B.A.; Lucy, M.C.

doi:10.1016/s0093-691x(99)00072-2

Cited by 49 publications

(46 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Serum was then collected by centrifugation (3000 £ g for 30 min at 4 8C), removed, and stored at 2 20 8C. Serum concentrations of IGF-I were determined by a double-antibody RIA procedure (Bilby et al 1999) with all the samples processed in a single assay. The primary antibody was obtained from Dr A F Parlow, National Hormone and Pituitary Program (UCLA Medical Center, Torrance, CA, USA).…”

Section: Igf-i Radioimmunoassay (Ria)mentioning

confidence: 99%

The IGF system in the neonatal ovine uterus

et al. 2005

View full text Add to dashboard Cite

Postnatal development of the ovine uterus primarily involves uterine gland morphogenesis or adenogenesis. Adenogenesis involves the budding differentiation of the glandular epithelium (GE) from the luminal epithelium (LE) and then GE proliferation and coiling/branching morphogenetic development within the stroma between birth (postnatal day or PND 0) and PND 56. Insulin-like growth factor (IGF)-I and IGF-II mRNAs were previously found to be expressed only in the endometrial stroma, whereas the IGF receptor (IGF-1R) mRNA was most abundant in epithelia and in stroma, suggesting that an intrinsic IGF system regulates postnatal development of the uterus. Given that the biological activities of IGFs are modulated by a family of six IGF binding proteins (IGFBPs) and specific proteases, the objective was to determine the effects of age and estrogen disruption on expression of IGFs, IGFBPs and pregnancy-associated plasma protein A (PAPP-A or IGFBP-4 protease) in the ovine uterus. In Study One, circulating levels of IGF-I and IGF-II in the serum of neonatal ewes did not change between PND 0 and PND 56. Levels of immunoreactive IGF-I, IGF-II and IGF-1R protein were most abundant on the apical surface of the endometrial LE and GE. RT-PCR analyses detected expression of IGFBPs (3, 4, 5 and 6) as well as PAPP-A mRNAs in the uterus, but not IGFBP-1 and IGFBP-2 mRNAs. IGFBP-3 and IGFBP-4 mRNAs were expressed specifically in the endometrial stroma and myometrium and increased after birth. PAPP-A mRNA was expressed specifically in the endometrial stroma and increased after birth. In Study Two, ewes were treated from birth with estradiol-17b valerate (EV), which reduces uterine growth and inhibits endometrial adenogenesis. On PNDs 14 and 56, IGFBP-3 mRNA was decreased in the uterus of EV-treated ewes, but IGF-1R and IGFBP-4 mRNAs were not affected. PAPP-A mRNA was increased by EV treatment on PND 14, but decreased on PND 56. These results support the hypothesis that an intrinsic IGF system in the uterus regulates epithelial-stromal interactions important for postnatal uterine growth and endometrial gland morphogenesis in the sheep.

show abstract

Section: Igf-i Radioimmunoassay (Ria)mentioning

confidence: 99%

The IGF system in the neonatal ovine uterus

et al. 2005

View full text Add to dashboard Cite

show abstract

“…It is important to mention that cows from this study received a subcutaneous application of bovine somatotropin (STB) as part of the herd management. The STB is a synthetic hormone with similar function to GH in bovine cattle, and its exogenous application is a potential enhancer of heat load in lactating cows under heat stress (13,30,31) , as well as blood concentrations of IGF1 (32)(33)(34)(35) . Therefore, administration of STB in this study could induce a direct effect on IGF1 levels, and consequently on PRL levels.…”

Section: Dinámica De Niveles Hormonales En Sangrementioning

confidence: 99%

“…Vale la pena señalar que las vacas de este estudio recibían la aplicación de un producto comercial como manejo habitual a base de somatotropina bovina (STB). La STB es una hormona sintética cuya función es similar a la GH en el bovino y su administración exógena incrementa potencialmente la carga de calor en ganado lactante bajo estrés calórico (13,30,31) , así como también la concentración de IGF1 en sangre (32,33,34,35) . Por lo tanto, la administración de STB en este trabajo pudo inducir un efecto directo sobre los niveles de IGF1 y consecuentemente en los de PRL.…”

Section: Dinámica De Niveles Hormonales En Sangreunclassified

Enfriamiento al inicio de verano para mitigar el estrés por calor en vacas Holstein del noroeste de México

Leyva-Corona

Thomas

Rincón

et al. 2016

Rev. Mex. Cienc. Pecu.

View full text Add to dashboard Cite

RESUMENEl objetivo de este estudio fue determinar la respuesta fisiológica, endócrina y productiva en vacas lactantes sometidas a varias series de enfriamiento artificial al inicio de verano (junio a julio de 2011). Se asignaron 14 vacas Holstein multíparas a uno de dos tratamientos: enfriamiento (E) antes de la ordeña (0700 y 1700 h) y enfriamiento adicional (EA) cada 2 h de 0700 a 1700 h en el corral de espera a la ordeña. Se midió la producción de leche (PL), niveles de prolactina (PRL) e IGF1, mientras que la temperatura rectal y frecuencia respiratoria fueron registradas y consideradas como indicadores fisiológicos de estrés por calor. La temperatura ambiental y humedad relativa también fueron registradas y utilizadas para calcular el índice de temperatura-humedad (ITH). Los indicadores de estrés calórico mostraron una interacción significativa (P<0.001) entre tratamiento y tiempo/semana, los cuales fueron menores con EA (-3.8 respiraciones por minuto y -0.2 °C) sólo durante las semanas 6 y 7. Los niveles séricos de PRL fueron mayores bajo enfriamiento adicional (EA: 35.1 vs E: 29.4 ng/ml; P=0.004), mientras que los de IGF1 solo variaron a través de las semanas de muestreo (P=0.014). La PL se incrementó (P<0.05) en EA durante las semanas 6 (+3.1 kg/día) y 7 (+3.1 kg/día). Se concluye que la mayor frecuencia de baños mitigó la condición de estrés calórico al inicio de verano, permitiéndole a la vaca expresar un mejor desempeño productivo al final del estudio, ya que dicho efecto dependió de la variable tiempo/semana. PALABRAS CLAVE: Enfriamiento, IGF1, Producción, Prolactina. ABSTRACTThe objective of this study was to determine the physiological, endocrine and productive response in lactating dairy cows under several series of artificial cooling at the beginning of the summer (June to July, 2011). Fourteen multiparous Holstein cows were assigned in one of two treatments: Cooling (C) before milking (0700 and 1700 h) and additional cooling (AC) each 2 h (from 0700 to 1700h) in the holding pen of the milking parlor. Milk yield (MY), and serum levels of prolactin (PRL) and IGF1 were measured. Rectal temperature and respiratory rate were registered and included as physiological markers of heat stress. Ambient temperature and relative humidity were also collected and used to calculate temperature-humidity index (THI). Both markers of heat stress showed a significant interaction (P<0.001) between treatment and time/week, which were lower in AC treatment (-3.8 breaths per minute and -0.2 °C) during wk 6 and 7. Serum levels of PRL were higher under additional cooling (AC: 35.1 vs C: 29.4 ng/ml; P=0.004), while IGF1 serum levels showed variation only through the sampling weeks (P=0.014). The MY increased (P<0.05) in AC only during wk 6 (+3.1 kg/d) and 7 (+3.1 kg/d). It was concluded that additional cooling at the beginning of the summer reduced heat stress in dairy cows allowing a better productive performance at the end of the study because such effect relied on the variable time/week. 416José Clemente Leyva-Corona...

show abstract

“…Plasma samples (n = 540) from 10 heifers and 10 steers of each purebred and crossbred breed group from each calf crop were removed from storage for determination of concentration of IGF-I by RIA as described by Bilby et al (1999). The protocol included 2 modifications.…”

Section: Igf-i Riamentioning

confidence: 99%

The influence of tropical adaptation on plasma concentrations of insulin-like growth factor-I in purebred and crossbred beef cattle1

Caldwell¹,

Chase²,

Riley³

et al. 2011

Journal of Animal Science

View full text Add to dashboard Cite

In an effort to determine whether tropical adaptation influences circulating concentrations of the growth-related hormone IGF-I, 3-breed diallel matings were conducted using temperate Bos taurus (Angus), tropical Bos indicus (Brahman), and tropical Bos taurus (Romosinuano). Purebred Angus, Braham, and Romosinuano and crossbred Angus-Braham, Angus-Romosinuano, and Braham-Romosinuano heifers and steers were evaluated in 2 separate calf crops from 2003 and 2004. Blood samples were obtained from 10 heifers of each breed group (n = 90) for each year at weaning and on d 0 and 84 of postweaning trials. Samples were also taken from 10 steers of each breed group (n = 90) at weaning and on d 0 and 60 of individual finishing phase feeding trials for each year. Concentrations of IGF-I were determined by RIA. Analyses included effects of sire breed, dam breed, year of record, the age of the dam of the calf in years, and interactions. Age of calf in days was investigated as a linear and quadratic covariate. Separate analyses were conducted for steers and heifers. The direct effect of Angus was to reduce (P < 0.03) heifer concentrations of IGF-I at d 84 and in the repeated measures analysis. In the repeated measures analysis, the direct effect of Romosinuano was to increase concentrations of IGF-I (P = 0.01). Relative to the temperate Bos taurus breed, plasma concentrations of IGF-I were numerically greater in male and female tropically adapted breed groups.

show abstract

Plasma gh, igf-i, and conception rate in cattle treated with low doses of recombinant bovine gh

Cited by 49 publications

References 30 publications

The IGF system in the neonatal ovine uterus

The IGF system in the neonatal ovine uterus

Enfriamiento al inicio de verano para mitigar el estrés por calor en vacas Holstein del noroeste de México

The influence of tropical adaptation on plasma concentrations of insulin-like growth factor-I in purebred and crossbred beef cattle1

Contact Info

Product

Resources

About