Plasma glucagon-like immunoreactivity (GLI) in dogs.

Ohneda, Akira; Horigome, Ken; Yanbe, Akira; Ishii, Soh; Itabashi, Hiroshi; Chiba, Masaki; Maruhama, Yoshisuke; Yamagata, Shoichi

doi:10.1620/tjem.115.337

Cited by 3 publications

(3 citation statements)

References 10 publications

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“…Although GLI used in the present study is in a stored form and not circulating form, it was reported that circulating GLI had the same molecular size as that in its tissue in origin (Valverde et al 1970, Ohneda et al 1975a. In the present experiment, GLI was infused into the pancreatic artery at a rate of 2 ng per minute.…”

Section: Discussionmentioning

confidence: 84%

“…GLI content in the fractions was determined by the method previously reported, using the anti-serum, G-7 (Oh neda et al 1972). This antiserum was produced in our laboratory against pork glucagon, and reacts with pancreatic glucagon as weil as GLI extracted from the intestine (Ohneda et al 1975a). Protein content in each eluate was determined by the method of Murphy and Kies (1960) and in part by absorption at 280 nm.…”

Section: Methodsmentioning

confidence: 99%

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Purification of Canine Gut Glucagon-Like Immunoreactivity (GLI) and Its Insulin Releasing Activity

Ohneda¹,

Horigome²,

Kai³

et al. 1976

Horm Metab Res

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In order to clarify the nature of the biological action of gut glucagon-like immunoreactivity (GLI), GLI was extracted from the mucosa of the canine intestine and purified by gel filtration and affinity chromatography. 1500 gm of the mucosa yielded approximately 7 gm of crude extract of GLI. This crude extract was applied to a column packed with Sephadex G-50 or Bio-Gel P-10 and two peaks were obtained, Peak I and II. Each peak was purified with affinity chromatography, bound to gamma-globulin of anti-glucagon rabbit-serum. In this step, the GLI was purified approximately 80 times in comparison with the crude extract. Peak I or Peak II, as well as pancreatic glucagon, was infused successively into the pancreaticoduodenal artery of the anesthetized dogs. When buffer solution or the Peak I GLI was infused, the plasma immunoreactive insulin in the pancreatic vein did not change significantly. In contrast, both the Peak II and pancreatic glucagon promoted insulin secretion from the pancreas. The results obtained in this experiment demonstrate the promotion of insulin release from the pancreas and suggest an important role of gut GLI in the absorption and metabolic processing of nutrients.

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Section: Discussionmentioning

confidence: 84%

Section: Methodsmentioning

confidence: 99%

Purification of Canine Gut Glucagon-Like Immunoreactivity (GLI) and Its Insulin Releasing Activity

Ohneda¹,

Horigome²,

Kai³

et al. 1976

Horm Metab Res

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“…Glycine is known as a potent secretagogue on glucagon release as well as arginine and methionine in dog (Ohneda et al 1973). Therefore, the slight increase in blood sugar level following gly¬ cine infusion may be considered as due to glucagon release.…”

Section: Discussionmentioning

confidence: 99%

Glycine stimulates growth hormone release in man

Kasai

Suzuki

Nakamura

et al. 1980

Acta Endocrinologica

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The influence of glycine, the simplest amino acid, on pituitary function has been investigated in the present study. Different doses (4, 8 or 12 g) of glycine were intravenously infused over 15 or 30 min in normal subjects. Serum levels of GH (growth hormone) and Prl (prolactin) were measured before and after the infusion, and also blood sugar levels were determined. The dose of 4 or 8 g glycine induced a significant increase in serum GH (P < 0.05 or P< 0.001, respectively); however, a more pronounced and significant increase in serum GH levels was observed after infusion at a dose of 12 g glycine (P < 0.001). It was clearly observed that the dose-dependent GH release to intravenous glycine occurred in normal subjects. On the contrary, serum Prl level was not changed significantly, and blood sugar level was transiently, but significantly (P < 0.05), increased after the infusion of 12 g glycine. The present data suggest that glycine might play an important role in the control of hypothalamic-pituitary function.

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Heterogeneity of Circulating Glucagon and Glucagon-Like Immunoreactivity

Valverde¹

1983

Glucagon I

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Plasma glucagon-like immunoreactivity (GLI) in dogs.

Cited by 3 publications

References 10 publications

Purification of Canine Gut Glucagon-Like Immunoreactivity (GLI) and Its Insulin Releasing Activity

Purification of Canine Gut Glucagon-Like Immunoreactivity (GLI) and Its Insulin Releasing Activity

Glycine stimulates growth hormone release in man

Heterogeneity of Circulating Glucagon and Glucagon-Like Immunoreactivity

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