Plasma Ischemia Modified Albumin Levels and Dynamic Thiol / Disulfide Balance in Sickle Cell Disease

Abstract

Objective:Sickle cell disease (SCD), described as a group of inherited blood disorders, affects millions of people throughout the world and is particularly common in the southern part of Turkey. We aimed to determine the relationship between ischemia-modified albumin (IMA) and the dynamic thiol/disulfide balance in SCD.Materials and Methods:Fifty-four adult SCD patients and 30 healthy controls were included in the study. The 54 adult patients included 30 (56%) males and 24 (44%) females with a mean age of 28.3±8.4 years (minimum-maximum: 18-46 years). Of the 54 patients, 46 had homozygous sickle cell anemia (HbSS) and 8 had sickle/β-thalassemia (HbS/β+-thalassemia). Fasting blood samples were collected. After centrifugation at 1500×g for 10 min, plasma samples were portioned and stored at -80 °C. IMA levels were determined by albumin cobalt binding test, a colorimetric method. Total and native thiols and disulfide were analyzed with a novel spectrophotometric method.Results:We found significantly lower levels of native thiol (-SH) (284.0±86.3 µmol/L), disulfide levels (14.6±7 µmol/L), and total thiols (-SH + -S-S-) (313.0±89.3 µmol/L) in SCD patients compared to healthy controls (respectively 417.0±54.2, 22.7±11.3, and 462.0±58.7 µmol/L). Plasma albumin levels (34.9±7.9 g/L) were lower and IMA levels (13.6±3.1 g/L) were higher in SCD patients compared to controls (respectively 43.5±3.1 and 8.4±1.6 g/L). Plasma albumin levels were strongly correlated with both plasma native (r=0.853; p=0.0001) and total thiols (r=0.866; p=0.0001).Conclusion:Decreased plasma native and total thiol levels and increased IMA levels are related to increased oxidative stress and provide an indirect and quick reflection of the oxidative damage in SCD patients.

Thiol/disulphide homeostasis in intensive care unit patients with sepsis and septic shock

“…Although it has been shown that thiols play a role in the pathogenesis of many disease, such as ankylosing spondylitis, lung cancer, rheumatoid arthritis, diabetic nephropathy, sickle cell disease, gastric cancer, and juvenile idiopathic arthritis [15][16][17][18][19][20][21], few studies have been conducted with respect to sepsis [22,23].…”

The Evaluation of Ischemia Modified Albumin and Adropin Levels in Patients with Pulmonary Embolism

“…Although the sensitivity of the D-dimer test is high, its specificity is low (24,27). In several studies, it was reported that IMA is a more sensitive test, compared with D-dimer, in the diagnosis of PE (28,29).…”

“…Ischemia-modified albumin (IMA) is formed by albumin modification caused by reactive oxygen derivatives due to ischemia. IMA is used as a sensitive biochemical marker of ischemia and oxidative stress caused by tissue hypoxia (23,24). In previous studies, it was reported that IMA levels increase among patients with ischemia, acute rheumatic fever, hepatic failure, chronic obstructive pulmonary disease (COPD) and cerebro-vascular disease.…”

Evaluation of dynamic thiol-disulfide homeostasis and ischemia-modified albumin levels in patients with chronic lymphocytic leukemia

“…Disulfide structures (S-S), which are formed as a result of OS are reduced to thiol groups again, and thiol balance is achieved 12. In many studies, the measurement of dynamic thiol disulfide balance has been shown as an indicator of increased free radical levels in various diseases 13–16. The increase in OS causes free radical damage at the N-terminal end of albumin molecules and decreases the binding capacity of albumin.…”

“…The increase in OS causes free radical damage at the N-terminal end of albumin molecules and decreases the binding capacity of albumin. The modified form of albumin is called ischemia-modified albumin (IMA) and is used a responsive marker of OS 13. To the best of our knowledge, this is the first study to evaluate both dynamic thiol-disulfide hemostasis and IMA levels in patients with CLL.…”