Plasma Level of Platelet-Derived Microparticles Is Associated with Coronary Heart Disease Risk Score in Healthy Men

Abstract

Aim:The aim of this study was to clarify the relationship between platelet-derived microparticles (PDMPs) and the Framingham 10-yr coronary heart disease (CHD) risk score. Methods: A cross-sectional study of healthy volunteers free of medication, and cardiovascular or cerebrovascular disease was conducted. The subjects were 190 Japanese men (median age 41). An ELISA kit and monoclonal antibodies against CD42b and CD42a (glycoprotein Ib and IX) were used. Results: PDMPs are correlated with platelet count, high sensitivity C-reactive protein (hsCRP), and diastolic blood pressure by multivariate analysis (R 2 0.316, p 0.001). Quartile range of PDMPs is significantly associated with the 10-yr CHD risk score after adjusting for age, platelet count, hsCRP, and hypertension (p 0.033) and for age, platelet count, hsCRP, and presence of metabolic syndrome (MS) (p 0.020). In individuals with a predicted 10-yr risk for CHD 8% (corresponding with the highest quartile), compared to those with a predicted 10-yr risk 8%, the odds ratio (OR), adjusted for age, platelet count, hsCRP, and hypertension, was 3.3 (1.2 8.9) and adjusted for age, platelet count, hsCRP, and MS, was 4.5 (1.6 11.8). The age-, platelet count-, hsCRP-and hypertensionadjusted OR for a 10-yr CHD risk score 8% was 0.8 (0.5 1.3) for hsCRP and 3.9 (1.6 9.4) for hypertension. The age-, platelet count-, hsCRP-and MS -adjusted OR for a 10-yr CHD risk score 8% was 0.7 (0.4 1.2) for hsCRP and 7.9 (2.6 24.5) for MS. Conclusion: Elevated PDMPs are associated with the 10-yr CHD risk score in healthy men. J Atheroscler Thromb, 2010; 17:342-349.

Association of the Plasma Platelet-Derived Microparticles to Platelet Count Ratio with Hospital Mortality and Disseminated Intravascular Coagulopathy in Critically Ill Patients

“…However, it is assumed that the PDMP level changes with the platelet count because PDMPs are released by platelets. Many previous reports have shown that there is a positive correlation between the platelet count and PDMP level as measured by ELISA and FCM in healthy individuals 38,39) . Moreover, Joop and coworkers used FCM and suggested that the PDMP level is simply a reflection of the platelet count 16) .…”

Leukocyte-Derived Microparticles in Vascular Homeostasis

“…137 The association between the Framingham risk score and the numbers of PMPs, LMPs, EPCMPs, and EMPs was evaluated. 126,142,143 From these studies, only 1 was prospective, including 488 patients with various CVD risk factors who were observed for a mean of 36 months. 142 These reports are promising.…”

Differences in Microparticle Release in Patients With Acute Coronary Syndrome and Stable Angina

“…5 They derive from various cells, most notably platelets, but also leucocytes, lymphocytes, erythrocytes, and endothelial cells. MP are present in a small amount in the circulation of healthy patients, 6 but their levels increase in patients with hypercoagulative states, including disseminated intravascular coagulation, 7 ACS, 8,9 peripheral artery disease (PAD), 10 diabetes mellitus, 11 and systemic inflammatory diseases. 12 Endothelial MP (EMP), in particular, are thought to be markers of endothelial injury and are released under stimulation by endothelium activating agents, such as complement fragments 13 or tumor necrosis factor alfa, 14 while platelet MP (PMP) are considered markers of platelet activation.…”