Plasma lipid and lipoprotein cholesterol concentrations in adult males consuming normal and high cholesterol diets under controlled conditions

Abstract

Twenty-three young adult males were fed diets containing either 400 or 1400 mg of cholesterol per day under controlled conditions for 4 wk. There were minimal differences between the two diets in total protein, carbohydrate, fat, and the P/S fatty acid ratio. In both diets 400 mg of cholesterol was supplied from nonegg food sources; the additional 1000 mg of cholesterol was from four whole eggs. Blood samples were collected after a 12- to 14-h fast at the beginning of the study, weekly throughout the experimental period, and 1 wk after completion of the study. Plasma total cholesterol and triglycerides and high-density, low-density, and very low-density lipoprotein cholesterol levels were measured. No significant differences in plasma total cholesterol, triglycerides, and lipoprotein cholesterol levels were observed between groups at any time. However, plasma cholesterol and cholesterol content of individual lipoproteins varied considerably among the individual subjects fed the high cholesterol diet. The importance of changes in the properties and metabolic activity of individual lipoproteins induced by dietary cholesterol with or without gross changes in the cholesterol levels remains to be determined.

Top-down and bottom-up lipidomic analysis of rabbit lipoproteins under different metabolic conditions using flow field-flow fractionation, nanoflow liquid chromatography and mass spectrometry

“…with controls. According to a study that investigated total TG levels from participants who were fed diets with either 1400 or 400 mg of cholesterol per day for a month, TG levels increased slightly but not as dramatically as 10-fold [26]. Similar result was observed from this study in agreement, in which levels of only 3 TG species were significantly altered (> 10-fold) by metabolic factors.…”

Cholesterol feeding increases low density lipoprotein synthesis.

“…In addition, excess sterol is reexcreted in the bile so that many individuals in this way are able to compensate to some extent for the increased cholesterol load (33). Undoubtedly this contributes to the recognised variability in the response of lipoprotein cholesterol to dietary sterol supplementation (20,28,29,33). Evidence for suppressed intestinal and hepatic LDL receptor activity in response to cholesterol feedinig is much more difficult to obtain.…”

“…The above procedure was repeated following 4 wk of high cholesterol feeding (6 eggs/d) when new steady-state LDL cholesterol levels would have been attained (28,29). The results obtained on the two occasions were compared by paired Student's t test.…”

Dietary cholesterol and cardiovascular disease: a systematic review and meta-analysis

“…Nineteen unique trials among 21 publications (8,(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56) reported outcome data on dietary cholesterol and serum cholesterol concentration. Seventeen of the 19 unique trials (8,(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)56) reported quantitative data and were included in metaanalysis ( Table 2).…”