2011
DOI: 10.1074/jbc.m111.236380
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Plasma Membrane Aquaporin AqpZ Protein Is Essential for Glucose Metabolism during Photomixotrophic Growth of Synechocystis sp. PCC 6803

Abstract: The genome of Synechocystis PCC 6803 contains a single gene encoding an aquaporin, aqpZ. The AqpZ protein functioned as a water-permeable channel in the plasma membrane. However, the physiological importance of AqpZ in Synechocystis remains unclear. We found that growth in glucose-containing medium inhibited proper division of ⌬aqpZ cells and led to cell death. Deletion of a gene encoding a glucose transporter in the ⌬aqpZ background alleviated the glucose-mediated growth inhibition of the ⌬aqpZ cells. The ⌬aq… Show more

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Cited by 23 publications
(27 citation statements)
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“…Synechocystis cells were grown axenically in sterilised BG-11 medium (nitrogen content, 18 mM; phosphorus content, 0.2 mM) [33] containing glucose (5 mM) with vigorous shaking at 120 rpm. [34] Nostoc cells were grown axenically in sterile BG-11 medium with orbital shaking, with or without air-bubbling. The cyanobacterial cells of each strain were incubated in 300 mL of medium in 500-mL Erlenmeyer flasks at 30 and 28 8C, with continuous illumination at 40 mmol photons m À2 s À1 .…”
Section: Methodsmentioning
confidence: 99%
“…Synechocystis cells were grown axenically in sterilised BG-11 medium (nitrogen content, 18 mM; phosphorus content, 0.2 mM) [33] containing glucose (5 mM) with vigorous shaking at 120 rpm. [34] Nostoc cells were grown axenically in sterile BG-11 medium with orbital shaking, with or without air-bubbling. The cyanobacterial cells of each strain were incubated in 300 mL of medium in 500-mL Erlenmeyer flasks at 30 and 28 8C, with continuous illumination at 40 mmol photons m À2 s À1 .…”
Section: Methodsmentioning
confidence: 99%
“…The AqpZ protein resides in the plasma membrane in the cell (1). Deletion of Synechocystis aquaporin aqpZ resulted in an increase in cell volume in the presence of low concentrations (5 mM) of glucose (1). This indicates that AqpZ plays a crucial role in the cellular osmotic adaptation to fluctuation of cytosolic osmotic pressure due to production and consumption of the photosynthetic products synthesized during the day.…”
mentioning
confidence: 94%
“…A P dnaK :: luxAB bioluminescent reporter strain (4, 24) was used as a control. Cells were grown to colonies on solid BG11 medium (1,42) at 30°C under white fluorescence lamps (42 mol of photons m Ϫ2 s Ϫ1 ), placed in the dark for 12 h to synchronize the circadian clock, and then kept under constant light. Bioluminescence from colonies was measured automatically every hour as described previously (35,36) using automated bioluminescence monitoring apparatuses (K. Okamoto, K. Onai, and M. Ishiura, unpublished data; models LL04-1 and CL24-W; Churitsu Electric Corp., Nagoya, Japan).…”
Section: Measurement Of Circadian Rhythm and Growth Rate Of Synechocymentioning
confidence: 99%
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