1995
DOI: 10.1021/bi00044a004
|View full text |Cite
|
Sign up to set email alerts
|

Plasma membrane caveolae mediate the efflux of cellular free cholesterol

Abstract: Caveolae are clathrin-free cell-surface organelles implicated in transmembrane transport. A fibroblast caveolar membrane fraction was isolated by sucrose density gradient ultracentrifugation and its identity confirmed by protein markers (caveolin, annexin II). When 3H-labeled free cholesterol was selectively transferred to the cells from labeled low density lipoprotein to increase cell free cholesterol approximately 15%, there was a 6-fold increase in label in the caveolar fraction above baseline levels. Subse… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

11
227
0
1

Year Published

1996
1996
2018
2018

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 275 publications
(239 citation statements)
references
References 31 publications
11
227
0
1
Order By: Relevance
“…Results from other investigations indicate that 1) caveolin is a cholesterol binding protein (Murata, 1995), and 2) a component of a cytosolic heat shock protein chaperone complex (Uittenbogaard, 1998). Caveolin also has been implicated in the intracellular cholesterol trafficking (Fielding, 1995;Smart, 1996) and has been claimed to be present in lipoprotein particles secreted by exocrine cells (Ping Sheng, 1999). These findings raise the possibility that EMTCs or subclasses of EMTCs are involved in the transport of caveolin from its site of synthesis to the plasmalemma, a possibility that deserves to be explored by future work.…”
Section: Discussionmentioning
confidence: 99%
“…Results from other investigations indicate that 1) caveolin is a cholesterol binding protein (Murata, 1995), and 2) a component of a cytosolic heat shock protein chaperone complex (Uittenbogaard, 1998). Caveolin also has been implicated in the intracellular cholesterol trafficking (Fielding, 1995;Smart, 1996) and has been claimed to be present in lipoprotein particles secreted by exocrine cells (Ping Sheng, 1999). These findings raise the possibility that EMTCs or subclasses of EMTCs are involved in the transport of caveolin from its site of synthesis to the plasmalemma, a possibility that deserves to be explored by future work.…”
Section: Discussionmentioning
confidence: 99%
“…Thyberg caveolin-containing vesicles in the trans-Golgi network or heat-shock protein-caveolin complexes in the cytoplasm, transported to the plasma membrane, and expelled extracellularly by transfer from caveolae to high-density lipoprotein or other acceptors (Fielding and Fielding, 1995Liscum and Munn, 1999;Uittenbogaard et al, 1998). The significance of this regulatory mechanism was affirmed by the finding Electron microscopy and demonstration of peroxidase activity in smooth muscle cells allowed to bind and internalize cholera toxin B subunit linked to horseradish peroxidase (CTB-HRP).…”
Section: Ldl Uptake and Cholesterol Distributionmentioning
confidence: 98%
“…Furthermore, caveolin binds cholesterol directly (Murata et al, 1995) and takes part in shuttling of free cholesterol between the endoplasmic reticulum (ER), the Golgi complex, and the cell surface (Conrad et al, 1995;Fielding and Fielding, 1996;Smart et al, 1996;Uittenbogaard et al, 1998). Efflux of cellular free cholesterol from cells is likewise believed to occur via caveolae (Fielding and Fielding, 1995). Clathrincoated pits constitute another functionally important part of the plasma membrane.…”
mentioning
confidence: 99%
“…The physical characteristics of the different cholesterol-induced interfacial packing densities in sphingoid-based and glycerol-based membrane lipids remain to be clearly defined but are likely to be important in modulating (i) detergent insolubility, (ii) the subcellular localization of certain integral membrane proteins (Bretscher & Munro, 1993) and proteins bearing GPIlipid anchors (Schroeder et al, 1994;Hanada et al, 1995), and (iii) the lateral distribution of complex gangliosides such as those known to associate with caveolae (Parton, 1994;Palestini et al, 1995). Given the tight packing density that occurs in sphingolipidcholesterol films, the rapid release of cholesterol from caveolae into the medium (Fielding & Fielding, 1995) is likely to be mediated by specific binding/transport proteins (Murata et al, 1995) that can translocate between the Golgi apparatus and surface caveolae (Smart et al, 1994).…”
Section: Physiological Implicationsmentioning
confidence: 99%