Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity

Li, Yujia; Jiao, Xiaoguo; Wang, Fengzhu; Huang, Xiangjuan; Gong, Ben-Qiang; Tao, Yuheng; Shen, Wenzhong; Tao, Kehan; Yao, Nan; Xiao, Shi; Zhou, Jian‐Min; Li, Jianfeng

doi:10.1038/s41477-022-01195-x

Cited by 38 publications

(25 citation statements)

References 81 publications

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“…Additionally, members of the RLCK-VII-4 subgroup, including PBL19 and PBL20, are involved in chitin-triggered MPK3/6, MPK4 activation, ROS production, defense gene expression, and redundantly confer basal resistance to the bacterial pathogen [ 19 , 20 ]. Moreover, Li et al [ 21 ] reported that after induction by chitin, a fraction of PBL19 protein translocates into the nucleus via the N-terminal nuclear localization sequence and induces transcriptional self-amplification primarily by WRKY8. Moreover, increased PBL19 proteins interact with EDS1 and promote antifungal immunity.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia

Maeda

Ackley

Yokotani

et al. 2023

IJMS

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Sugarcane smut caused by Sporisorium scitamineum is one of the most devastating sugarcane diseases. Furthermore, Rhizoctonia solani causes severe diseases in various crops including rice, tomato, potato, sugar beet, tobacco, and torenia. However, effective disease-resistant genes against these pathogens have not been identified in target crops. Therefore, the transgenic approach can be used since conventional cross-breeding is not applicable. Herein, the overexpression of BROAD-SPECTRUM RESISTANCE 1 (BSR1), a rice receptor-like cytoplasmic kinase, was conducted in sugarcane, tomato and torenia. BSR1-overexpressing tomatoes exhibited resistance to the bacteria Pseudomonas syringae pv. tomato DC3000 and the fungus R. solani, whereas BSR1-overexpressing torenia showed resistance to R. solani in the growth room. Additionally, BSR1 overexpression conferred resistance to sugarcane smut in the greenhouse. These three BSR1-overexpressing crops exhibited normal growth and morphologies except in the case of exceedingly high levels of overexpression. These results indicate that BSR1 overexpression is a simple and effective tool for conferring broad-spectrum disease resistance to many crops.

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Section: Discussionmentioning

confidence: 99%

Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia

Maeda

Ackley

Yokotani

et al. 2023

IJMS

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“…PTI and ETI function cooperatively and potentiate each other (43,44), but the mechanisms that interconnect PTI and ETI remain unclear. The EDS1/PAD4/ADR1 module associates with some PM located PRRs and is required for PTI (45)(46)(47). NbNRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4 recognizing the apoplastic fungal effector Avr4 (48).…”

Section: Effector Activation Of a Tnl Induces Atnrg11 Resistosome For...mentioning

confidence: 99%

Plasma membrane association and resistosome formation of plant helper immune receptors

Wang

Liu

et al. 2023

Preprint

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Intracellular plant immune receptors, termed NLRs, respond to pathogen effectors delivered into plant cells. Activation of NLRs typically confers strong immunity. Sensor NLRs, involved in effector recognition, are either TIR-NLRs (TNLs) or CC-NLRs (CNLs). Helper NLRs, required for sensor NLR signaling, include CCR-NLRs (RNLs) and a special class of CNLs known as NRCs. Activated TNLs produce small molecules that induce an association between the EDS1/SAG101 heterodimer and the NRG1s helper RNLs. Auto active NRG1s oligomerize and form calcium signaling channels largely localized at the plasma membrane (PM). The molecular mechanisms of helper NLR PM association and effector induced NRG1 oligomerization remain uncharacterized. We find that both RNLs and NRCs require positively charged residues in the second and fourth helices of their CCR or CC domain for phospholipid binding and PM association before and after activation, despite conformational changes that accompany activation. We demonstrate that effector activation of TNLs induces NRG1 oligomerization at the PM and the cytoplasmic pool of EDS1/SAG101 is critical for cell death function. EDS1/SAG101 are absent from the oligomerized NRG1 resistosome, suggesting that additional unknown triggers might be required to induce the dissociation of EDS1/SAG101 from the NRG1/EDS1/SAG101 heterotrimer before subsequent NRG1 oligomerization. Our data provide new observations regarding dynamic PM association during helper NLR activation and underpin an updated model for effector induced NRG1 resistosome formation.

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“…In addition, another study found that the RLCK PBL19, after metacaspase processing, specifically interacts with and phosphorylates EDS1 protein after treatment of fungal chitin in Arabidopsis [ 29 ]. The phosphorylated EDS1 protein accumulates in the cytoplasm and contributes to plant antifungal immunity ( Fig 1 ) [ 29 ]. Whether the phosphorylated EDS1-mediated signaling is dependent on PAD4 or SAG101 remains to be determined.…”

Section: The “Eds1-helper Nlr Complex” As a Key Intersecting Point Of...mentioning

confidence: 99%

Plant immune receptor pathways as a united front against pathogens

2023

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Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity

Cited by 38 publications

References 81 publications

Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia

Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia

Plasma membrane association and resistosome formation of plant helper immune receptors

Plant immune receptor pathways as a united front against pathogens

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