Plasma miR-150-5p as a Biomarker for Chronic Obstructive Pulmonary Disease

Abstract

Purpose To investigate the potential of plasma microRNA-150-5p (miR-150-5p) as a biomarker for chronic obstructive pulmonary disease (COPD) and its relationship with clinical indicators such as pulmonary function. Patients and Methods Fifty-nine patients with COPD and twenty-six healthy control individuals were recruited in the Second People’s Hospital of Hefei from September 2021 to September 2022. The plasma expression level of miR-150-5p was measured by quantitative real-time polymerase chain reaction. Results The miR-150-5p level in the COPD group was significantly lower than that in the control group, and the relative expression was lower in patients with severe airflow limitation than those with mild limitation. Plasma miR-150-5p levels were positively correlated with pulmonary function indicators and negatively correlated with the white blood cell count and C-reactive protein level. The receiver operating characteristic curve suggested that plasma miR-150-5p had predictive value for COPD (area under curve = 0.819, sensitivity 64.4%, specificity 92.3%). Conclusion MiR-150-5p can be useful for the diagnosis and disease assessment of COPD, and has value as a biomarker for COPD.

Blood miRNAs as Potential Diagnostic Biomarkers for Chronic Obstructive Pulmonary Disease: A Meta-Analysis

“…of COPD Patients Age Sex (Male/Female) No. of Control Age Sex (Male/Female) Sample Type Test Method Normalizer Chen et al 17 2019 China English 104 SCOPD: 67.53±8.49, AECOPD: 65.13±9.87 88/16 50 63.47±11.31 41/9 Plasma qRT-PCR U6 Ding et al 18 2023 China English 59 75.12 ± 7.85 50/9 26 71.65 ± 6.35 20/6 Plasma qRT-PCR Cel-miR-39-3p Gao et al 19 2022 China Chinese 164 SCOPD: 67.97±8.24, AECOPD: 69.12±7.89 107/57 82 68.27±8.22 57 /25 Plasma qRT-PCR U6 Geng et al 20 2020 China English 53 60.32± 6.35 32/21 50 59.83±5.29 25/25 Serum qRT-PCR U6 Jiang et al …”

“…These criteria involved excluding reviews, in vitro and in vivo experiments, as well as articles with sample sources other than blood or those with unusable data. In conclusion, a total of 16 eligible studies [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] were incorporated into the meta-analysis, comprising 8 English-language publications and 8 Chinese-language publications. The publication period of the included studies spans from 2013 to 2023.…”

Maternal hsa-miR-423-5p associated with the cognitive development of babies in pregnant women without mental disorders

“…At this time, as an initial precaution, we quantified the samples using the Spectrophotometer (Thermo Scientific™ NanoDrop™) to evaluate the purification of the extracted RNA. Regarding endogenous control and duplicate reactions, we did not find standardization in the literature, including studies published in recent years using a single endogenous miRNA for control ( Sekovanić et al, 2021 ; Bayati et al, 2022 ; Couto et al, 2022 ; Li et al, 2022 ; Ding et al, 2023 ; Higashi et al, 2023 ). Furthermore, Couto et al (2022) cite duplicate reactions and the other authors do not cite the number of reactions carried out.…”

Exploration of the Pathogenesis of Chronic Obstructive Pulmonary Disease Caused by Smoking—Based on Bioinformatics Analysis and In Vitro Experimental Evidence

“…For example, Ouyang et al found that miR-185-5p and miR-182-5p were involved in the development of COPD and might serve as potential biomarkers for the diagnosis of COPD [ 7 ]. Ding et al also found that miR-150-5p could be used for the diagnosis and disease assessment of COPD [ 8 ]. The expression levels of miR-203a-3p and miR-375 were significantly higher in both COPD patients and healthy people with smoking [ 9 ].…”