Plasma pK1 and Bicarbonate Concentration

Flear, C. T. G.; Hayes, Shawn G.; Stoddart, J.C.; Covington, A.K.

doi:10.1042/cs067047pa

Cited by 11 publications

(19 citation statements)

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“…An earlier study found very good agreement between serum HCO 3 Ϫ values obtained with a Beckman Astra analyzer and those calculated from measurements taken with a Radiometer blood gas instrument (2 ). Thus, the degree of agreement between measured and calculated HCO 3 Ϫ values is likely a function of the particular methods used within the laboratory. Some authors have also suggested that the pK value for HCO 3 Ϫ may vary substantially with the patient population (3,4 ).…”

Section: To the Editormentioning

confidence: 87%

“…We also reproduced common preanalytical errors in the laboratory to evaluate their effect on the rela- 3 Ϫ concentration calculated from the results obtained with an Instrumentation Laboratory blood gas analyzer (1 ). An earlier study found very good agreement between serum HCO 3 Ϫ values obtained with a Beckman Astra analyzer and those calculated from measurements taken with a Radiometer blood gas instrument (2 ). Thus, the degree of agreement between measured and calculated HCO 3 Ϫ values is likely a function of the particular methods used within the laboratory.…”

Section: To the Editormentioning

confidence: 95%

“…HCO 3 Ϫ values are also frequently calculated from pH and CO 2 partial pressure measurements obtained during arterial blood gas (ABG) analysis. To determine whether measured and calculated HCO 3 Ϫ values can be used interchangeably and to determine whether a comparison of measured and calculated HCO 3 Ϫ values can be used to detect errors in the collection or analysis of blood samples, we compared measured (venous) and calculated (arterial) HCO 3 Ϫ concentrations for arterial and venous samples drawn at the same time. We also reproduced common preanalytical errors in the laboratory to evaluate their effect on the rela- 3 Ϫ concentration calculated from the results obtained with an Instrumentation Laboratory blood gas analyzer (1 ).…”

Section: To the Editormentioning

confidence: 99%

“…To determine whether measured and calculated HCO 3 Ϫ values can be used interchangeably and to determine whether a comparison of measured and calculated HCO 3 Ϫ values can be used to detect errors in the collection or analysis of blood samples, we compared measured (venous) and calculated (arterial) HCO 3 Ϫ concentrations for arterial and venous samples drawn at the same time. We also reproduced common preanalytical errors in the laboratory to evaluate their effect on the rela- 3 Ϫ concentration calculated from the results obtained with an Instrumentation Laboratory blood gas analyzer (1 ). An earlier study found very good agreement between serum HCO 3 Ϫ values obtained with a Beckman Astra analyzer and those calculated from measurements taken with a Radiometer blood gas instrument (2 ).…”

Section: To the Editormentioning

confidence: 99%

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Comparison of Measured and Calculated Bicarbonate Values

Kumar

Karon²

2008

Clinical Chemistry

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Section: To the Editormentioning

confidence: 87%

Section: To the Editormentioning

confidence: 95%

Section: To the Editormentioning

confidence: 99%

Section: To the Editormentioning

confidence: 99%

See 2 more Smart Citations

Comparison of Measured and Calculated Bicarbonate Values

Kumar

Karon²

2008

Clinical Chemistry

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“…The study of Flear et al 14 showed that pKa values varied considerably by more than 0.06 in healthy volunteers and in very ill patients and hence, an error of approximately ± 60% was noted in calculated bicarbonate based on constant pKa of 6.1. Another study by O'Leary and Langton found a significant decrease of the pKa in patients considered to have metabolic acidosis when compared with patients with bicarbonate concentration within the reference interval.…”

Section: Discussionmentioning

confidence: 99%

Comparison of measured Serum Total Carbon Dioxide with calculated Bicarbonate calculated from Arterial Blood Gas Analysis

Mohan¹,

Kumar²

2017

Indian Journal of Medical Biochemistry

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Introduction Measured total carbon dioxide (TCO2) from venous sample and calculated bicarbonate from arterial blood gas (ABG) have shown good agreement in some studies, while conflicting results have been obtained in few other studies. The objective of this study is to compare and assess the degree of agreement between the measured TCO2 and calculated bicarbonate and also whether they can be used interchangeably in our laboratory. Materials and methods We prospectively analyzed 89 ABG samples requested for calculated bicarbonate and then measured TCO2 from venous blood samples drawn simultaneously from the same participants between November 2016 and April 2017. Results Measured TCO2 results ranged from 5.7 to 39.9 mmol/L (mean 23.45 mmol/L), while calculated bicarbonate ranged from 9 to 40 mmol/L (mean 24.36 mmol/L). The values of TCO2 and bicarbonate correlated well (r = 0.95, p < 0.001), with the correlation given by the equation, y = 0.884x + 3.605. The bias obtained was —0.9 mmol/L and the standard deviation (SD) was 1.62 mmol/L. The limits of agreement (LOA) were —4.1 to 2.3 mmol/L, with a span of 6.4 mmol/L. Out of the 89 values, 85 (95.05%) were within the LOA. Conclusion In majority of the cases, the calculated bicarbonate concentration from ABG showed a good correlation to the measured venous TCO2 concentration. Despite this excellent correlation, TCO2 did not show good agreement with calculated bicarbonate when Story and Poustie's criteria were applied, especially in cases of bicarbonate less than 20 mmol/L. Hence, clinicians should be aware of this discrepancy and be cautious when using measured TCO2 and calculated bicarbonate interchangeably in the assessment and management of acid—base disorders, especially in patients with metabolic acidosis. How to cite this article Mohan T, Kumar BV. Comparison of measured Serum Total Carbon Dioxide with calculated Bicarbonate calculated from Arterial Blood Gas Analysis. Indian J Med Biochem 2017;21(2):76-80.

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Measuring Equilibrium Bicarbonate Concentrations Directly in Cellular Mitochondria and in Human Serum Using Europium/Terbium Emission Intensity Ratios

Smith

Pál

Parker

2012

Chemistry A European J

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A series of Eu and Tb complexes of four different chiral ligands incorporating an azaxanthone sensitiser has been evaluated as probes for the bicarbonate anion. Their binding affinities were assessed at ambient pH with bicarbonate, lactate, citrate, phosphate and serum albumin. Binding was signalled by modulation of circularly polarised luminescence and apparent affinity constants were measured by examining changes in emission intensity ratios. Competition experiments show that with these species and ATP present at normal physiological values, bicarbonate can be determined selectively over the concentration range 10 to 35 mM. Bicarbonate levels are also reported by using a mixture of Eu and Tb complexes of a common ligand, examining the ratio of red/green emitted light. These methods have been adapted for the determination of bicarbonate in human serum and used for the assessment of mitochondrial levels of bicarbonate in several different cell types with confocal microscopy.

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Plasma pK1 and Bicarbonate Concentration

Cited by 11 publications

References 0 publications

Comparison of Measured and Calculated Bicarbonate Values

Comparison of Measured and Calculated Bicarbonate Values

Comparison of measured Serum Total Carbon Dioxide with calculated Bicarbonate calculated from Arterial Blood Gas Analysis

Measuring Equilibrium Bicarbonate Concentrations Directly in Cellular Mitochondria and in Human Serum Using Europium/Terbium Emission Intensity Ratios

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