Plasma protein-bound di-tyrosines as biomarkers of oxidative stress in end stage renal disease patients on maintenance haemodialysis

Colombo, Graziano; Reggiani, Francesco; Cucchiari, David; Portinaro, Nicola; Giustarini, Daniela; Rossi, Ranieri; Garavaglia, Maria Lisa; Saino, Nicola; Milzani, Aldo; Badalamenti, Salvatore; Dalle‐Donne, Isabella

doi:10.1016/j.bbacli.2016.12.004

Cited by 17 publications

(20 citation statements)

References 84 publications

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“…Classical oxidative stress and carbonyl, nitrosative, and chlorine stress were widely studied in many diseases [4, 11–13]. The pathogenic effect of oxidative stress involves enhancing of proatherosclerosis mechanisms and cardiovascular risk in patients with CKD [14].…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, AOPP should be recognized as one of a general biomarkers of oxidative stress because their molecular composition have been so far poorly classified [2, 3]. This group of modified proteins includes dityrosine (di-Tyr), pentosidine, and carbonyl-containing protein products (reactive C=O) [3, 4]. Furthermore, it probably influences reproducibility and accuracy of spectrophotometric measurement of their concentration based on reaction with chloramine T at 340 nm [5].…”

Section: Introductionmentioning

confidence: 99%

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Advanced Oxidation Protein Products and Carbonylated Proteins as Biomarkers of Oxidative Stress in Selected Atherosclerosis-Mediated Diseases

Gryszczyńska

Formanowicz

Budzyń

et al. 2017

BioMed Research International

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Objectives The main question of this study was to evaluate the intensity of oxidative protein modification shown as advanced oxidation protein products (AOPP) and carbonylated proteins, expressed as protein carbonyl content (C=O) in abdominal aortic aneurysms (AAA), aortoiliac occlusive disease (AIOD), and chronic kidney disease (CKD). Design and Methods The study was carried out in a group of 35 AAA patients and 13 AIOD patients. However, CKD patients were divided into two groups: predialysis (PRE) included 50 patients or hemodialysis (HD) consisted of 34 patients. AOPP and C=O were measured using colorimetric assay kit, while C-reactive protein concentration was measured by high-sensitivity assay (hsCRP). Results The concentration of AOPP in both AAA and AIOD groups was higher than in PRE and HD groups according to descending order: AAA~AIOD > HD > PRE. The content of C=O was higher in the PRE group in comparison to AIOD and AAA according to the descending order: PRE~HD > AAA~AIOD. Conclusions AAA, AIOD, and CKD-related atherosclerosis (PRE and HD) contribute to the changes in the formation of AOPP and C=O. They may promote modification of proteins in a different way, probably due to the various factors that influence oxidative stress here.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advanced Oxidation Protein Products and Carbonylated Proteins as Biomarkers of Oxidative Stress in Selected Atherosclerosis-Mediated Diseases

Gryszczyńska

Formanowicz

Budzyń

et al. 2017

BioMed Research International

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“…Creatinine, C-reactive protein, white blood cell count, albumin, fibrinogen, haemoglobin, ferritin, total iron-binding capacity, urea, sodium, potassium, calcium, and phosphorus were measured by standardized methods at the clinical laboratory of the Humanitas Clinical and Research Center [ 12 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…S -thiolated plasma proteins, measured as protein thiolation index (PTI) [ 14 ], are indeed increased in ESRD patients on maintenance HD [ 12 , 13 ]. Other biomarkers of protein oxidation in haemodialysed patients are plasma protein-bound dityrosines [ 15 , 16 ] as well as plasma protein carbonyls (PCO), whose levels are elevated compared to healthy subjects [ 17 – 19 ]. Similarly, biomarkers of inflammation are elevated in haemodialysed patients [ 20 – 22 ].…”

Section: Introductionmentioning

confidence: 99%

Plasma Protein Carbonylation in Haemodialysed Patients: Focus on Diabetes and Gender

Colombo

Reggiani

Cucchiari

et al. 2018

Oxidative Medicine and Cellular Longevity

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Patients with end-stage renal disease (ESRD) undergoing haemodialysis (HD) experience oxidative/carbonyl stress, which is postulated to increase after the HD session. The influence of diabetes mellitus and sex on oxidation of plasma proteins in ESRD has not yet been clarified despite that diabetic nephropathy is the most common cause of ESRD in developed and developing countries and despite the increasingly emerging differences between males and females in epidemiology, pathophysiology, clinical manifestations, and outcomes for several diseases. Therefore, this study aimed to evaluate the possible effect of type 2 diabetes mellitus, gender, and dialysis filter on plasma level of protein carbonyls (PCO) in ESRD patients at the beginning and at the end of a single HD session. Results show that mean post-HD plasma PCO levels are significantly higher than mean pre-HD plasma PCO levels and that the type of dialysis filter and dialysis technique are unrelated to plasma PCO levels. The mean level of plasma PCO after a HD session increases slightly but significantly in nondiabetic ESRD patients compared to diabetic ones, whereas it increases more markedly in women than in men. These novel findings suggest that women with ESRD are more susceptible than men to oxidative/carbonyl stress induced by HD.

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“…Dityrosine cross-links can be formed both intra- and intermolecularly [ 17 , 18 ] by the o,o’ coupling of two tyrosinyl radicals. Dityrosine cross-links have been viewed as markers of oxidative stress in vivo [ 19 , 20 , 21 , 22 ], although there is evidence that they might play a causal role in age-related pathologies such as Alzheimer’s [ 23 ] and Parkinson’s disease [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Reversible Oxidative Modifications in Myoglobin and Functional Implications

et al. 2020

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Myoglobin (Mb), an oxygen-binding heme protein highly expressed in heart and skeletal muscle, has been shown to undergo oxidative modifications on both an inter- and intramolecular level when exposed to hydrogen peroxide (H2O2) in vitro. Here, we show that exposure to H2O2 increases the peroxidase activity of Mb. Reaction of Mb with H2O2 causes covalent binding of heme to the Mb protein (Mb-X), corresponding to an increase in peroxidase activity when ascorbic acid is the reducing co-substrate. Treatment of H2O2-reacted Mb with ascorbic acid reverses the Mb-X crosslink. Reaction with H2O2 causes Mb to form dimers, trimers, and larger molecular weight Mb aggregates, and treatment with ascorbic acid regenerates Mb monomers. Reaction of Mb with H2O2 causes formation of dityrosine crosslinks, though the labile nature of the crosslinks broken by treatment with ascorbic acid suggests that the reversible aggregation of Mb is mediated by crosslinks other than dityrosine. Disappearance of a peptide containing a tryptophan residue when Mb is treated with H2O2 and the peptide’s reappearance after subsequent treatment with ascorbic acid suggest that tryptophan side chains might participate in the labile crosslinking. Taken together, these data suggest that while exposure to H2O2 causes Mb-X formation, increases Mb peroxidase activity, and causes Mb aggregation, these oxidative modifications are reversible by treatment with ascorbic acid. A caveat is that future studies should demonstrate that these and other in vitro findings regarding properties of Mb have relevance in the intracellular milieu, especially in regard to actual concentrations of metMb, H2O2, and ascorbate that would be found in vivo.

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Plasma protein-bound di-tyrosines as biomarkers of oxidative stress in end stage renal disease patients on maintenance haemodialysis

Cited by 17 publications

References 84 publications

Advanced Oxidation Protein Products and Carbonylated Proteins as Biomarkers of Oxidative Stress in Selected Atherosclerosis-Mediated Diseases

Advanced Oxidation Protein Products and Carbonylated Proteins as Biomarkers of Oxidative Stress in Selected Atherosclerosis-Mediated Diseases

Plasma Protein Carbonylation in Haemodialysed Patients: Focus on Diabetes and Gender

Reversible Oxidative Modifications in Myoglobin and Functional Implications

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