Plasma protein values in infants

Hickmans, Evelyn M.; Finch, Ernest; Tonks, E. L.

doi:10.1136/adc.18.94.96

Cited by 21 publications

(3 citation statements)

References 1 publication

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“…These low levels confirm the observations made by Darrow and Cary (1933) and by Rapoport, Rubin, and Chaffee (1943). Other previous observers (Utheim, 1920;Bridge, Cohen, and McNair Scott, 1941;Hickmans, Finch, and Tonks, 1943) have found that the serum protein levels of premature infants are lower than those of full-term infants in the early weeks of life, but few details regarding the weight at birth and none about the diet of the infants are given in their reports. The premature infants who were the subjects in this part of our investigation were all receiving the high protein and relatively high calorie diet of the second period and therefore their low serum protein levels (compared with those of full-term infants) are unlikely to have been due to malnutrition.…”

Section: Resultssupporting

confidence: 79%

Protein Requirements of Infants: 3. The Nutrition of Premature Infants

Young¹,

Poyner-Wall²,

Humphreys³

et al. 1950

Archives of Disease in Childhood

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Section: Resultssupporting

confidence: 79%

Protein Requirements of Infants: 3. The Nutrition of Premature Infants

Young¹,

Poyner-Wall²,

Humphreys³

et al. 1950

Archives of Disease in Childhood

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“…The plasma total protein is predominantly comprised of albumin and immunoglobulins (reference ranges between 60–80 mg/ml 25–27 ). Cases had significantly lower total plasma protein concentration (median; 59.9 mg/ml, IQR; 51.4–70) compared to controls (median; 67.3 mg/ml, IQR 60.1–74.8) ( P < 0.001, Fig.…”

Section: Resultsmentioning

confidence: 99%

Biomarkers of post-discharge mortality among children with complicated severe acute malnutrition

Njunge

Gwela

Kibinge

et al. 2019

Sci Rep

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High mortality after discharge from hospital following acute illness has been observed among children with Severe Acute Malnutrition (SAM). However, mechanisms that may be amenable to intervention to reduce risk are unknown. We performed a nested case-control study among HIV-uninfected children aged 2–59 months treated for complicated SAM according to WHO recommendations at four Kenyan hospitals. Blood was drawn from 1778 children when clinically judged stable before discharge from hospital. Cases were children who died within 60 days. Controls were randomly selected children who survived for one year without readmission to hospital. Untargeted proteomics, total protein, cytokines and chemokines, and leptin were assayed in plasma and corresponding biological processes determined. Among 121 cases and 120 controls, increased levels of calprotectin, von Willebrand factor, angiotensinogen, IL8, IL15, IP10, TNFα, and decreased levels of leptin, heparin cofactor 2, and serum paraoxonase were associated with mortality after adjusting for possible confounders. Acute phase responses, cellular responses to lipopolysaccharide, neutrophil responses to bacteria, and endothelial responses were enriched among cases. Among apparently clinically stable children with SAM, a sepsis-like profile is associated with subsequent death. This may be due to ongoing bacterial infection, translocated bacterial products or deranged immune response during nutritional recovery.

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“…[1][2][3][4][5][6][7][8][9] However, there is only sparse information on the cerebrospinal fluid protein fractions in this age group.10-18 The concentration of the low protein content in small amounts of cerebrospinal fluid has presented a major problem. [1][2][3][4][5][6][7][8][9] However, there is only sparse information on the cerebrospinal fluid protein fractions in this age group.10-18 The concentration of the low protein content in small amounts of cerebrospinal fluid has presented a major problem.…”

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Protein Studies in Normal Newborn Infants

Piliero¹,

Lending²

1959

AMA Am J Dis Child

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Serum protein fractions have been studied intensively in the normal newborn infant.1-9 However, there is only sparse information on the cerebrospinal fluid protein fractions in this age group.10-18 The concentration of the low protein content in small amounts of cerebrospinal fluid has presented a major problem. Earlier studies primarily used precipitation methods which could not separate the individual fractions. The use of dialysis methods for concentrating proteins, combined with paper electrophoresis, now affords adequate means for separation of the various components of cerebrospinal fluid proteins.Paper electrophoretic studies of cerebrospinal fluid have been performed for the most part only in adults and older children,19-22 with few reports in normal newborn infants.17,18 The purpose of this study was to determine the distribution and relationship of proteins in the cerebrospinal fluid and serum of normal newborn infants utilizing chemical and paper electrophoretic methods. MethodThirty-five normal newborn infants ranging in age from 2\ m=1/ 2\ 2 hours to 114 hours were studied.All were products of full-term uneventful gestation and deliveries and on subsequent observations for at least two months were apparently normal.Approximately 3 ml. of cerebrospinal fluid were obtained by lumbar puncture, centrifuged immedi¬ ately to remove any erythrocytes, and kept in a frozen state until processed. Blood samples were obtained by femoral venipuncture, allowed to clot for approximately one hour, and centrifuged, and serum was removed for analysis.The concentration of total protein in the serum and cerebrospinal fluid was determined by a modi¬ fication of the Biuret method.23 In preparation for paper electrophoresis, 2 to 3 ml. portions of cere¬ brospinal fluid were concentrated in an Oxford Model dialyzer for three hours against a 30% solution of polyvinyl pyrolidone (P. V. P.). Eight strips of Whatman 3 MM filter paper were run simultaneously. Barbital (Veronal) buffer at pH 8.6 and 0.05 ionic strength was used. After initial equilibration, serum or cerebrospinal fluid was ap¬ plied at the apex of the wet strip, 10 microliter portions of serum and 100 microliter portions of the cerebrospinal fluid concentrate were applied in duplicate. A constant current of 6-9 ma. was em¬ ployed for 16-hour runs. After drying at 90-120 C for 30 minutes, strips were stained with amidoschwarz 10B dye solution for the protein distribu¬ tion pattern. Densitometry was performed with a Spinco Servo-Type integrating scanner. In this type of scanner, the area under the density curves is shown by a corresponding series of pips. For each component, the Gaussian distribution curve was approximated and lines were drawn through the intersections of the curves for adjacent com¬ ponents. ResultsThe cerebrospinal fluid proteins occurred in the electrophoretic patterns as five bands, depicited in Figure 2 with their correspond¬ ing paper strip, in the order of increasing mobility to -, ß-, a2-, and ¿^-globulins and to albumin. In addition to the...

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Plasma protein values in infants

Cited by 21 publications

References 1 publication

Protein Requirements of Infants: 3. The Nutrition of Premature Infants

Protein Requirements of Infants: 3. The Nutrition of Premature Infants

Biomarkers of post-discharge mortality among children with complicated severe acute malnutrition

Protein Studies in Normal Newborn Infants

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