Plasma proteome analysis in diet‐induced obesity‐prone and obesity‐resistant rats

Choi, Jung-Won; Wang, Xia; Joo, Jeong In; Kim, Dong Hyun; Oh, Tae Seok; Choi, Duk Kwon; Yun, Jong Won

doi:10.1002/pmic.201000391

Cited by 32 publications

(28 citation statements)

References 74 publications

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“…In our previous studies, we found that male rats are more susceptible to obesity when fed a HFD, and many proteins play roles in obesity resistance in different metabolic tissues [13]–[16]. In conjunction with the present data, an earlier report demonstrated the anti-obesity effects of CAV1, as evidenced by reduced Cav1 expression in visceral adipose tissue of obese patients [10].…”

Section: Introductionsupporting

confidence: 89%

Sex-Dependent Expression of Caveolin 1 in Response to Sex Steroid Hormones Is Closely Associated with Development of Obesity in Rats

et al. 2014

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Caveolin-1 (CAV1) is a conserved group of structural membrane proteins that form special cholesterol and sphingolipid-rich compartments, especially in adipocytes. Recently, it has been reported that CAV1 is an important target protein in sex hormone-dependent regulation of various metabolic pathways, particularly in cancer and diabetes. To clarify distinct roles of CAV1 in sex-dependent obesity development, we investigated the effects of high fat diet (HFD) and sex steroid hormones on CAV1 expression in adipose tissues of male and female rats. Results of animal experiments revealed that estrogen (17-β-estradiol, E2) and androgen (dihydrotestosterone, DHT) had opposite effects on body weight gain as well as on the regulation of CAV1, hormone sensitive lipase (HSL) and uncoupling protein 1 (UCP1) in adipose tissues. Furthermore, sex hormone receptors and aromatase were differentially expressed in a sex-dependent manner in response to E2 and DHT treatments. In vivo data were confirmed using 3T3-L1 and HIB1B cell lines, where Cav1 knock down stimulated lipogenesis but suppressed sex hormone receptor signaling proteins. Most importantly, co-immunoprecipitation enabled the identification of previously unrecognized CAV1-interacting mitochondrial or lipid oxidative pathway proteins in adipose tissues. Taken together, current data showed that CAV1 may play important preventive role in the development of obesity, with more prominent effects in females, and proved to be an important target protein for the hormonal regulation of adipose tissue metabolism by manipulating sex hormone receptors and mitochondrial oxidative pathways. Therefore, we can report, for the first time, the molecular mechanism underlying the effects of sex steroid hormones in the sex-dimorphic regulation of CAV1.

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Section: Introductionsupporting

confidence: 89%

Sex-Dependent Expression of Caveolin 1 in Response to Sex Steroid Hormones Is Closely Associated with Development of Obesity in Rats

et al. 2014

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“…Microarray analysis revealed differences in expression of genes encoding metabolic enzymes in adipose tissue from OP and OR rats (13). Plasma proteome analysis showed changes related to inflammation and oxidative stress (14). We confirmed accelerated weight gain, increased adiposity, and altered metabolic parameters in OP rats.…”

Section: Discussionsupporting

confidence: 62%

Cardiovascular, metabolic, and coronary dysfunction in high‐Fat‐Fed obesity‐resistant/prone rats

Dake

Oltman

2015

Obesity

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Objective: Obesity is a global epidemic leading to several comorbidities including diabetes and cardiovascular disease. The hypothesis that the genetic background of the obesity-prone rat (OP) predisposes to physiologic, metabolic, and microvascular dysfunction which is exacerbated by a diet high in saturated fats was tested. Methods: Male OP and obesity-resistant (OR) rats were fed either a diet containing 10% (chow) or 45% kcal fat (HF) for 42 weeks. Results: Weight of OP rats was greater than OR rats by 8 weeks on both diets. Blood pressure was increased in OP rats on chow and further augmented by HF diet compared to OR rats on similar diets. In contrast to weight and blood pressure, glucose clearance was similar in OR and OP rats on chow and impaired in both models on HF diet. Relaxation to acetylcholine was attenuated in OP rats compared to OR rats by 8 weeks and remained reduced throughout the study. A longer period of time was required to observe vascular dysfunction in HF-fed OR rats. Conclusions: When compared to OR rats, OP rats are prone to develop not only greater obesity but also hypertension and vascular dysfunction on a normal diet which is further augmented with HF diet.

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“…Of the 16 differentially expressed proteins identified in this study, 14 have already been associated with other chronic diseases in rats [28], humans [18-22,24,29,30], and horses [26] using DIGE. To our knowledge, coagulation factor X and IgA have not been associated with either acute or chronic diseases using DIGE based proteomics.…”

Section: Discussionmentioning

confidence: 99%

Plasma proteomics shows an elevation of the anti-inflammatory protein APOA-IV in chronic equine laminitis

Steelman

Chowdhary

2012

BMC Vet Res

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BackgroundEquine laminitis is a devastating disease that causes severe pain in afflicted horses and places a major economic burden on the horse industry. In acute laminitis, the disintegration of the dermal-epidermal junction can cause the third phalanx to detach from the hoof wall, leaving the horse unable to bear weight on the affected limbs. Horses that survive the acute phase transition into a chronic form of laminitis, which is often termed “founder”. Some evidence suggests that chronic laminar inflammation might be associated with alterations in the endocrine and immune systems. We investigated this broad hypothesis by using DIGE to assess global differences in the plasma proteome between horses with chronic laminitis and controls.ResultsWe identified 16 differentially expressed proteins; the majority of these were involved in the interrelated coagulation, clotting, and kininogen cascades. Clinical testing of functional coagulation parameters in foundered horses revealed a slight delay in prothrombin (PT) clotting time, although most other indices were within normal ranges. Upregulation of the intestinal apolipoprotein APOA-IV in horses with chronic laminitis was confirmed by western blot.ConclusionsOur results support the hypothesis that localized laminar inflammation may be linked to systemic alterations in immune regulation, particularly in the gastrointestinal system. Gastrointestinal inflammation has been implicated in the development of acute laminitis but has not previously been associated with chronic laminitis.

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Plasma proteome analysis in diet‐induced obesity‐prone and obesity‐resistant rats

Cited by 32 publications

References 74 publications

Sex-Dependent Expression of Caveolin 1 in Response to Sex Steroid Hormones Is Closely Associated with Development of Obesity in Rats

Sex-Dependent Expression of Caveolin 1 in Response to Sex Steroid Hormones Is Closely Associated with Development of Obesity in Rats

Cardiovascular, metabolic, and coronary dysfunction in high‐Fat‐Fed obesity‐resistant/prone rats

Plasma proteomics shows an elevation of the anti-inflammatory protein APOA-IV in chronic equine laminitis

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