Plasma Proteomics Analysis of Dairy Cows with Milk Fever Using SELDI-TOF-MS

Sheng, Shi; Xia, Cheng; Zhang, Hongyou; Sun, Zhaolei; Liu, Jiannan; Wang, Bo

doi:10.3923/ajava.2014.1.12

Cited by 3 publications

(3 citation statements)

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“…Previous studies on CH and SH in dairy cows reported that the occurrence of CH is related to blood coagulation and complement pathways [3, 21–23], and our results are consistent with this observation. A number of differentially expressed proteins identified in the present study were related to blood coagulation and complement pathways, specifically platelet factor, serpin and complement factor.…”

Section: Discussionsupporting

confidence: 93%

“…This disease increases the risk of other perinatal diseases due to the reduced blood Ca concentration and muscle contractive capacity [1]. SH has been studied using proteomics approaches based on SELDI-TOF-MS (surface-enhanced laser desorption/ionization-time of flight-Mass spectrometry) [2, 3], 2D-DIGE (fluorescence two-dimensional differential gel electrophoresis) combined with MALDI-TOF-MS (matrix-assisted laser desorption/ionization-time of flight-mass spectrometry) [4] and metabolomics based on 1 H-NMR (1hydrogen-nuclear magnetic resonance) [5]. However, these techniques have their limitation on studying differentially expressed proteins in dairy cows with SH.…”

Section: Resultsmentioning

confidence: 99%

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Protein profiling of plasma proteins in dairy cows with subclinical hypocalcaemia

Fan

Sheng

et al. 2017

Ir Vet J

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Subclinical hypocalcaemia (SH) is an important metabolic disease in dairy cows that has a serious impact on production performance. The objective of this study was to investigate novel aspects of pathogenesis using proteomics technology to identify proteins that are differentially expressed in diseased and healthy animals. Dairy cows were divided into an SH group (T, n = 10) and a control group (C, n = 10) based on plasma calcium concentration. A total of 398 differentially expressed proteins were identified, of which 265 proteins were overlapped in the two parallel experiments. Of these, 24 differentially expressed proteins were statistically significant. Gene Ontology analysis yielded 74 annotations, including 7 cellular component, 55 biological process and 12 molecular function categories. Bioinformatics analysis indicated that calcium regulation, immune and inflammatory response, blood coagulation and complement pathway were all related to SH. Our iTRAQ/LC-MS/MS (isobaric tags for relative and absolute quantification/liquid chromatography-mass spectrometry/mass spectrometry) approach proved highly effective for plasma protein profiling of dairy cows with SH, and the results pave the way for further studies in this area.

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Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Protein profiling of plasma proteins in dairy cows with subclinical hypocalcaemia

Fan

Sheng

et al. 2017

Ir Vet J

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“…In fact, many proteins are known to affect Ca regulation in dairy cows. Shu and Wang reported 12 differentially expressed proteins related to CH and SH by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows

Sheng

Bai

Wang

et al. 2016

Asian-Australas J Anim Sci

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ObjectiveHypocalcemia is an important metabolic disease of dairy cows during the transition period, although the effect of hypocalcemia on biological function in dairy cows remains unknown.MethodsIn this study, proteomic, mass spectrum, bioinformatics and western blotting were employed to identify differentially expressed proteins related to serum Ca concentration. Serum samples from dairy cows were collected at three time points: 3rd days before calving (day −3), the day of calving (day 0), and 3rd days after calving (day +3). According to the Ca concentration on day 0, a total of 27 dairy cows were assigned to one of three groups (clinical, subclinical, and healthy). Samples collected on day −3 were used for discovery of differentially expressed proteins, which were separated and identified via proteomic analysis and mass spectrometry. Bioinformatics analysis was performed to determine the function of the identified proteins (gene ontology and pathway analysis). The differentially expressed proteins were verified by western blot analysis.ResultsThere were 57 differential spots separated and eight different proteins were identified. Vitamin D-binding protein precursor (group-specific component, GC), alpha-2-macroglobulin (A2M) protein, and apolipoprotein A-IV were related to hypocalcemia by bioinformatics analysis. Due to its specific expression (up-regulated in clinical hypocalcemia and down-regulated in subclinical hypocalcemia), A2M was selected for validation. The results were consistent with those of proteomic analysis.ConclusionA2M was as an early detection index for distinguishing clinical and subclinical hypocalcemia. The possible pathogenesis of clinical hypocalcemia caused by GC and apolipoprotein A-IV was speculated. The down-regulated expression of GC was a probable cause of the decrease in calcium concentration.

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Milk Fever: Reductionist Versus Systems Veterinary Approach

Dervishi

Ametaj

2017

Periparturient Diseases of Dairy Cows

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Plasma Proteomics Analysis of Dairy Cows with Milk Fever Using SELDI-TOF-MS

Cited by 3 publications

References 28 publications

Protein profiling of plasma proteins in dairy cows with subclinical hypocalcaemia

Protein profiling of plasma proteins in dairy cows with subclinical hypocalcaemia

Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows

Milk Fever: Reductionist Versus Systems Veterinary Approach

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