Plasma proteomics data from hibernating and active Scandinavian brown bears

Frøbert, Anne Mette; Gregersen, Simon; Brohus, Malene; Welinder, Karen G.; Kindberg, Jonas; Fröbert, Ole; Overgaard, Michael Toft

doi:10.1016/j.dib.2022.107959

Cited by 1 publication

(2 citation statements)

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“…Whether this is due to low plasma concentrations, post-translational modifications, or due to poor peptide ionization efficiency is not known. No difference in the TTR mRNA level was observed in agreement with the two proteomics studies [21,22]. The albumin mRNA level increased to ~ 120% during hibernation in agreement with previously measured protein levels [13,21,23].…”

Section: Discussionsupporting

confidence: 90%

“…In the grizzly bear liver transcript data by Jansen et al [ 13 ], we observed that the mRNA level of TBG, the major thyroid hormone carrier, increased to ~ 450% in hibernation compared to the active state. TBG was not identified in two mass spectrometry-based proteomics studies from our group (ProteomeXchange datasets PXD003946 and PXD030482) despite being included in the database used for protein identification [ 21 , 22 ]. Whether this is due to low plasma concentrations, post-translational modifications, or due to poor peptide ionization efficiency is not known.…”

Section: Discussionmentioning

confidence: 99%

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Hypothyroidism in hibernating brown bears

Frøbert

Nielsen²,

Brohus³

et al. 2023

Thyroid Res

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Brown bears hibernate throughout half of the year as a survival strategy to reduce energy consumption during prolonged periods with scarcity of food and water. Thyroid hormones are the major endocrine regulators of basal metabolic rate in humans. Therefore, we aimed to determine regulations in serum thyroid hormone levels in hibernation compared to the active state to investigate if these are involved in the adaptions for hibernation.We used electrochemiluminescence immunoassay to quantify total triiodothyronine (T3) and thyroxine (T4) levels in hibernation and active state in paired serum samples from six subadult Scandinavian brown bears. Additionally, we determined regulations in the liver mRNA levels of three major thyroid hormone-binding proteins; thyroxine-binding globulin (TBG), transthyretin (TTR), and albumin, by analysis of previously published grizzly bear RNA sequencing data.We found that bears were hypothyroid when hibernating with T4 levels reduced to less than 44% (P = 0.008) and T3 levels reduced to less than 36% (P = 0.016) of those measured in the active state. In hibernation, mRNA levels of TBG and albumin increased to 449% (P = 0.031) and 121% (P = 0.031), respectively, of those measured in the active state. TTR mRNA levels did not change.Hibernating bears are hypothyroid and share physiologic features with hypothyroid humans, including decreased basal metabolic rate, bradycardia, hypothermia, and fatigue. We speculate that decreased thyroid hormone signaling is a key mediator of hibernation physiology in bears. Our findings shed light on the translational potential of bear hibernation physiology to humans for whom a similar hypometabolic state could be of interest in specific conditions.

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