Plasma quality after whole‐blood filtration depends on storage temperature and filter type

Abstract

The study evaluated the quality of plasma obtained after whole-blood filtration with four different polyester filters and one polyurethane filter. The activities of coagulation factors and proteinase inhibitors were not or only negligibly affected by filtration, in all experiments. Filtration did not increase markers of clotting and fibrinolysis. Only a strong neutrophil and complement activation was observed, which depended on the type of filter and whole-blood storage conditions. However, as neutrophil elastase-specific degradation products did not increase and the complement-derived anaphylatoxin C3a was found in its inactivated form, C3a-desArg, these filtration-dependent changes apparently have little impact on the therapeutic quality of whole-blood-filtered fresh frozen plasma for transfusion.

Plasma and cryoprecipitate manufactured from whole blood held overnight at room temperature meet quality standards

“…The plasma is effectively leukoreduced by centrifugation in the BC method and thus no filtration step is included in the method. Some WB leukoreduction filters have been associated with small decreases of FVIII activity 9‐11 although this does not appear to be true of all filters 10,12 . The filters used in the WBF method were not the subject of these studies.…”

The effect of different methods of leucoreduction on plasma coagulation factors

“…Many published European and American studies have investigated the effect of leucofiltration of either whole blood or plasma on residual clotting factors. Heiden et al [18], used five different whole blood filters, four polyester and one polyurethane for leucofiltration. They found no statistically significant reductions in clotting factors postfiltration.…”

Coagulation factor content of plasma produced from whole blood stored for 24 hours at ambient temperature: results from an international multicenter BEST Collaborative study

“…After ambient storage of whole blood for 24 hours there was no loss of activity of coagulation factors FV, FVII, FXI, FXII, and FXIII and only a small (<5%) loss of FII, FIX, and FX. Others have shown that FV, FXI, and FXII are unaltered after storage of whole blood at ambient temperature for 16 to 18 hours 13,14 …”

“…The operational and economic advantages to blood services of ambient storage of whole blood for 24 hours are large, due to removing the restriction of having to process blood to make PLTs or plasma within 8 hours of collection. Although there have been a number of studies examining plasma produced from whole blood stored for 24 hours at ambient temperature, 12‐14 few studies have directly compared this to plasma produced on the day of whole blood collection with a large number of units and across a range of coagulation factors.…”