Plasma responses of pancreatic polypeptide, glucagon and insulin in normal and alloxan diabetic dogs, and their regional levels in the pancreas

Abstract

The plasma responses of pancreatic polypeptide (PP), glucagon (IRG) and insulin (IRI) after administration of beef soup were studied in normal and alloxan diabetic dogs kept in a poor metabolic state for 4 weeks, and their regional levels in the pancreas were determined and compared at the uncinate process, head, body and tail. The plasma PP levels of the diabetic dogs were significantly higher than those of the normal dogs and they increased after beef soup administration in both groups. The plasma IRG levels did not change significantly after beef soup administration in either group, but significantly high levels were found in the diabetic dogs. The IRI content in the normal dog pancreas was highest at the tail, followed in order by the body, head and uncinate process, and was decreased to about one-tenth to one-fortieth in the alloxan diabetic dogs. On the other hand, the amounts of PP in the pancreas of both normal and diabetic dogs were greatest at the uncinate process, followed in order by the head, body and tail. In an inverse relationship with the PP findings, the IRG content in the pancreas was highest at the tail and lowest at the uncinate process in both kinds of dogs. The differences in both PP and IRG between diabetic and normal dogs were generally not significant. These results show that irrespective of the high plasma levels of PP and IRG in the diabetic dogs, their levels in the pancreas did not change significantly.

Contents and secretion of glucagon and insulin in rat pancreatic islets from the viewpoint of their localization in pancreas.

“…The IRG content was high in the gastric and splenic parts, and very low in the duodenal part. Similar findings were also reported in the canine pancreas ( Gingerich et al 1978;Tasaka et al 1984). Orci et al (1976) and Baetens et al (1976) found morphologically using the immunofluorescence technique that glucagon containing cells were abundant in the body and tail of the pancreas, and that, on the other hand, PP-containing cells were abundant in the head and scarce in the body and tail of the rat pancreas.…”

“…The contents of insulin and glucagon in the pancreas have been reported to be rich in the dorsal lobe compared with those in the ventral lobe (Wrenshall et al 1952: Orci andPerrelet 1981;Trimble et al 1982;Tasaka et al 1984). On the contrary, pancreatic polypeptide is abundant in the ventral lobe of the pancreas (Gingerich et al 1978;Gersell et al 1979;Orci and Perrelet 1981;Tasaka et al 1984).…”

“…On the contrary, pancreatic polypeptide is abundant in the ventral lobe of the pancreas (Gingerich et al 1978;Gersell et al 1979;Orci and Perrelet 1981;Tasaka et al 1984).…”

Insulin, glucagon and somatostatin content of the non-obese diabetic (NOD) mouse pancreas and plasma virus antibodies to Coxsackie B- and reoviruses.

“…Hyperglucagonemia and high glucagon content in the pancreas are found in streptozotocin diabetic rats together with a marked decrease in insulin in the pancreas (unpublished observations) and no significant increase in glucagon content in the pancreas of alloxan diabetic dogs was reported (Tasaka et al 1984). The histological findings in the pancreas of NOD mice would be different from those in animals with such experimental diabetes in that the destruction of the pancreatic islet is not limited to B cells.…”