Plasma Rich in Growth Factors for the Treatment of Dry Eye after LASIK Surgery

Sánchez-Ávila, Ronald Mauricio; Merayo‐Lloves, Jesús; Fernández, María; Rodríguez-Gutiérrez, Luis Alberto; Jurado, Nancy; Muruzábal, Francisco; Orive, Gorka; Anitua, Eduardo

doi:10.1159/000487951

Cited by 22 publications

(19 citation statements)

References 29 publications

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“…[8][9][10] In recent years, PRGF eye drops have been widely used in the treatment of several ocular surface diseases, including dry eye, corneal ulcers, and persistent epithelial defects. [11][12][13][14][15][16][17][18] The biochemical and biophysical properties of PRGF eye drops like pH, osmolarity, proteins, and growth factors content, which are similar to the artificial tears, are responsible for the successful results obtained in the treatment of these different ocular pathologies. 19,20 Ocular surface disorders are usually chronic diseases that require medium-or long-term treatment.…”

Section: Introductionmentioning

confidence: 99%

Development and Optimization of Freeze-Dried Eye Drops Derived From Plasma Rich in Growth Factors Technology

Anitua

Fuente

Alcalde

et al. 2020

Trans. Vis. Sci. Tech.

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To investigate whether plasma rich in growth factors (PRGF) eye drops maintain their biological potential after a freeze drying process. The addition of a lyoprotectant like trehalose was also evaluated. Methods: Blood from three healthy donors was collected to obtain eye drops by PRGF technology. The resultant eye drops were divided in four groups: PRGF, freeze-dried PRGF (PRGF lyo), and PRGF lyophilized mixed with 2,5% trehalose (PRGF lyo+2.5T) or 5% trehalose (PRGF lyof+5T). Chemical and biological characteristics were evaluated. Photorefractive keratectomy was performed on C57BL/6 mice which were divided in three treatment groups: control, PRGF, and PRGF lyo. Corneal wound healing and haze formation were evaluated macroscopically. Eyes were collected at 1, 2, 3, and 7 days after surgery, and were processed for histologic studies. Results: The pH values of PRGF samples increased significantly after the lyophilization process. Osmolarity levels increased significantly in PRGF samples mixed with trehalose in comparison with PRGF samples without protectants. The freeze drying process maintained growth factors levels as well as the biological properties of PRGF eye drops even without the use of lyoprotectants. PRGF lyo treatment significantly decreased the re-epithelialization time and haze formation in photorefractive keratectomy-treated corneas regarding PRGF and control groups. Furthermore, the PRGF lyo group significantly decreased the number of smooth muscle actin-positive cells in comparison with the control group at each time of the study and at days 2 and 3 in the PRGF group. Conclusions: The freeze drying process preserves the protein and growth factor content as well as the biological properties of PRGF eye drops, even without the use of protectants. Freeze-dried PRGF eye drops accelerate corneal tissue regeneration after photorefractive keratectomy in comparison with the control group. Translational Relevance: Our study shows the feasibility to preserve the biological capability of PRGF eye drops as freeze-dried formulation, avoiding the addition of protectants. Resumen (Foreign Language Abstract): Objetivo: Investigar si el colirio de plasma rico en factores de crecimiento (PRGF) mantiene su potencial biológico después de un proceso de liofilización. También se evaluó la adición de un lioprotector como la trehalosa. Métodos: Se recogió sangre de tres donantes sanos para obtener colirio mediante la tecnología PRGF. El colirio resultante se dividió en cuatro grupos: PRGF, PRGF liofilizado (PRGF lyo) y PRGF liofilizado mezclado con trehalosa al 2,5% (PRGF lyo + 2.5T) o trehalosa al 5% (PRGF lyof + 5T). Se evaluaron las características químicas y biológicas de cada uno de los colirios. Se realizó una queratectomía fotorrefractiva (PRK) en ratones C57BL/6 que se dividieron en tres grupos de tratamiento: Control, PRGF y PRGF lyo. La curación de la herida corneal y la formación de "haze"se evaluaron macroscópicamente.

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Section: Introductionmentioning

confidence: 99%

Development and Optimization of Freeze-Dried Eye Drops Derived From Plasma Rich in Growth Factors Technology

Anitua

Fuente

Alcalde

et al. 2020

Trans. Vis. Sci. Tech.

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“…Dry eye disease (DED) presents a multifactorial pathology at the ocular surface, including tear film changes with or without corneal damage, ocular symptoms, visual degradation, and increased tear osmolarity, which together lead to a reduction in quality of life [1]. DED is the most common complication of corneal refractive surgery [2,3] and is the main cause of refractive regression, decline in vision quality, and decreased patient satisfaction [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Relationship between lipid layer thickness, incomplete blinking rate and tear film instability in patients with different myopia degrees after small-incision lenticule extraction

Zhou³

et al. 2020

PLoS ONE

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Objective To elucidate the relationship between lipid layer thickness (LLT), incomplete blinking rate and tear film stability in patients with different myopia degrees after small-incision lenticule extraction (SMILE) and to determine whether there is a difference in the prevalence of dry eye disease (DED) after SMILE among patients with different myopia degrees. Methods Fifty patients (100 eyes) were enrolled in this study; they were divided into 3 groups according to the degree of spherical refraction: a low-myopia group (LMG; spherical refraction �-3.00 D, 20 eyes), a moderate-myopia group (MMG;-3.00 D < spherical refraction <-6.00 D, 40 eyes), and a high-myopia group (HMG; spherical refraction ≧-6.00 D, 40 eyes). Testing indicators included the ocular surface disease index (OSDI), fluorescein tear film breakup time (FBUT), corneal fluorescence staining (CFS), the Schirmer test (SI), lipid layer thickness (LLT), blink rate (BR) per 20 seconds, incomplete blinking rate, noninvasive keratograph assessment of first and average tear film breakup time (NIKBUT f , NIKBUT av), and tear meniscus height (TMH). Each indicator was evaluated preoperatively and postoperatively at 1 w, 1 mo and 3 mo. Results The mean age was 29.12±5.95 years. There were no significant differences among the three groups (p>0.05), except preoperative age (p = 0.006). There were significant differences in the FBUT among the three groups at postoperative 1 w and 1 mo (p<0.05). There were significant differences in the incomplete blinking rate and FBUT between the LMG and the HMG at postoperative 1 mo (p<0.05). The number of first tear film breakup points located beyond the 6 mm diameter of the cornea was higher in the HMG than in the other PLOS ONE

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“…[ 3 ] Its effectiveness has also been demonstrated for the treatment of dry eye after LASIK. [ 24 ] In vivo studies carried out in animal models for PRK, it has been observed that PRGF increases the proliferation of corneal epithelial cells, and reduces the number of SMA positive cells (responsible for myofibroblast differentiation), which corresponds to greater corneal transparency for evaluation by microscopy. In such a way that PRGF accelerates the regeneration of corneal tissue after PRK and diminishes the formation of haze.…”

Section: Discussionmentioning

confidence: 99%

Plasma rich in growth factors versus Mitomycin C in photorefractive keratectomy

et al. 2021

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To evaluate the efficacy and safety of plasma rich in growth factors (PRGF) in photorefractive keratectomy (PRK) versus Mitomycin C (MMC). This is a comparative, longitudinal and retrospective case-control study (MMC vs PRGF), in patients with a spherical correction from −0.25 to −8.00 D and cylinder correction from −0.25 to −3.00. The uncorrected distance visual acuity (UDVA), refractive efficacy and safety indices, and changes in endothelial cell density were evaluated. The predictability was assessed with the postoperative manifest spherical equivalent. Forty-four patients (72 eyes) were treated with MMC and twenty-five patients (45 eyes) with PRGF. The final UDVA (LogMar) in MMC was 0.029 ± 0.065 and in PRGF it was 0.028 ± 0.048 (p = 0.383). The efficacy index for MMC was 0.98 ± 0.10 and 1.10 ± 0.46 for patients treated with PRGF (p = 0.062). The safety index for MMC was 1.03 ± 0.11 and 1.12 ± 0.46 (p = 0.158) for PRGF group. The change percentage of endothelial cell density was 0.9 ± 11.6 for MMC and 4.3 ± 13.1 for PRGF (p = 0.593). The predictability for MMC was 92.1% and for the PRGF was 91.9% (p = 0.976). Hyperemia, eye pain and superficial keratitis were observed in 11.1% of the MMC group; no adverse events were observed with the PRGF. The use of PRGF in PRK surgery is as effective as MMC. The PRGF shows a better safety profile than MMC for its intraoperative use in PRK.

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Plasma Rich in Growth Factors for the Treatment of Dry Eye after LASIK Surgery

Cited by 22 publications

References 29 publications

Development and Optimization of Freeze-Dried Eye Drops Derived From Plasma Rich in Growth Factors Technology

Development and Optimization of Freeze-Dried Eye Drops Derived From Plasma Rich in Growth Factors Technology

Relationship between lipid layer thickness, incomplete blinking rate and tear film instability in patients with different myopia degrees after small-incision lenticule extraction

Plasma rich in growth factors versus Mitomycin C in photorefractive keratectomy

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