Plasma-treated PET surfaces improve the biocompatibility of human endothelial cells

Ramires, P. A.; Mirenghi, L.; Romano, Antonio; Palumbo, Fabio; Nicolardi, Giuseppe

doi:10.1002/1097-4636(20000905)51:3<535::aid-jbm31>3.0.co;2-p

Cited by 116 publications

(72 citation statements)

References 14 publications

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“…On the plasma treated surfaces, cell proliferation was shown to be improved in comparison to the untreated surfaces. Moreover, the nitrogen-containing plasma-treated surfaces proved to be the best substrates for endothelial cell proliferation compared to surfaces that were treated exclusively with oxygen or hydrogen plasma [55].…”

Section: Nitrogen-containing Moietiesmentioning

confidence: 99%

Active screen plasma nitriding enhances cell attachment to polymer surfaces

Kaklamani

Bowen

Mehrban

et al. 2013

Applied Surface Science

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Active Screen Plasma Nitriding (ASPN) is a well-established technique used for the surface modification of materials, the result of which is often a product with enhanced functional performance. Here we report the modification of the chemical and mechanical properties of ultra-high molecular weight poly(ethylene) (UHMWPE) using 80:20 (v/v) N 2 /H 2 ASPN, followed by growth of 3T3 fibroblasts on the treated and untreated polymer surfaces. ASPN-treated UHMWPE showed extensive fibroblast attachment within three hours of seeding, whereas fibroblasts did not successfully attach to untreated UHMWPE. Fibroblast-coated surfaces were maintained for up to 28 days, monitoring their metabolic activity and morphology throughout. The chemical properties of the ASPN-treated UHMWPE surface were studied using X-ray photoelectron spectroscopy, revealing the presence of C-N, C=N, and C≡N chemical bonds. The elastic modulus, surface topography, and adhesion properties of the ASPN-treated UHMWPE surface were studied over 28 days during sample storage under ambient conditions and during immersion in two commonly used cell culture media. KeywordsActive screen plasma nitriding, atomic force microscopy, fibroblast, interferometry, nanoindentation, X-ray photoelectron spectroscopy 2 1. Introduction Biomaterials that will be used for medical applications are required to have excellent bulk and surface properties to resist mechanical deformation while providing a surface that enables tissue attachment. Designing a material that exhibits good bulk properties together with the appropriate surface characteristics to enable biological integration is often challenging [1][2][3]. Polymeric materials exhibit attractive characteristics for biomedical applications. They are of low density, are easy to process and of relatively low cost. It has also been reported that polymers can be tailored to influence the viability, growth and function of attached cells controlling the cell function by the chemical, morphological and mechanical properties of the polymeric surface [4]. The surface properties of polymers, however, do not often satisfy the requirements for biomedical applications such as scratch resistance, wettability, biocompatibility, gas permeability and friction. They often appear to have low surface energy and poor adhesive properties hence surface modification is required [5][6][7][8].

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Section: Nitrogen-containing Moietiesmentioning

confidence: 99%

Active screen plasma nitriding enhances cell attachment to polymer surfaces

Kaklamani

Bowen

Mehrban

et al. 2013

Applied Surface Science

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“…[7,8,32] Moreover, NH 2 surface chemistry was already shown to induce increased phosphorylation of FAK, in comparison with CH 3 , OH, or COOH chemistries. [33] The effect of NH 2 on cell attachment is believed to be due to the positively-charged surface (NH 3 þ ) which forms in aqueous media, and thus influences serumprotein adsorption and subsequent adhesion of cells onto the aminated surfaces.…”

Section: Human Umbilical Vein Endothelial Cells (Huvecs) Morphology Amentioning

confidence: 99%

Nitrogen‐Rich Plasma‐Polymerized Coatings on PET and PTFE Surfaces Improve Endothelial Cell Attachment and Resistance to Shear Flow

Gigout

Ruíz

Wertheimer

et al. 2011

Macromolecular Bioscience

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Low seeding efficiency and poor cell retention under flow-induced shear stress limit the effectiveness of in vitro endothelialization strategies for small-diameter vascular grafts. Primary-amine-rich plasma-polymerized coatings (PPE:N) deposited using low- and atmospheric-pressure plasma discharges on PET and PTFE are evaluated for their ability to improve endothelial cells' kinetics and strength of attachment. PPE:N coatings increase cell adhesion and adhesion rate, spreading, focal adhesion, and resistance to flow-induced shear compared with bare and gelatin-coated PET and PTFE. In particular, about 90% of the cells remain on coated surfaces after 1 h exposure to shear. These coatings, therefore, appear as a promising versatile approach to improve cell seeding strategies for vascular grafts.

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“…We therefore sought to compare the effect of plasma treatments on the surface modification of PET fibres and PET films, with emphasis on ammonia plasma treatments that promote the formation of surface amino groups which are recognised as improving cell adhesion and, [24][25][26] more importantly, may potentially also be used for further biomolecule conjugation [27,28]. To this end, we examined the dependence between PET structure geometry and plasma treatment duration.…”

Section: Introductionmentioning

confidence: 99%

Low Pressure Radio Frequency Ammonia Plasma Surface Modification on Poly(ethylene terephthalate) Films and Fibers: Effect of the Polymer Forming Process

Öteyaka

Chevallier

Turgeon

et al. 2011

Plasma Chem Plasma Process

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We investigated the effect of a polyethylene terephthalate (PET) forming process on radiofrequency ammonia plasma surface-treated PET flat films and fibres obtained by melt blowing. Ammonia plasma treatment allowed for the incorporation of amino functionalities on both the film and fibre surfaces, with higher values observed at very short treatment times. This plasma treatment also induced polymer chain scissions which were observed as the formation of hydrophilic nodules that coalesced together and were loosely bound to the underlying polymeric materials. These plasma-induced surface damages were notably more important on the melt-blown PET fibres. Consequently, maximisation of the surface amino groups with minimal polymer chain breaking was achieved using very short plasma treatment times (typically 1 s). We also demonstrated that the polymer forming process must be taken into account when plasma modifications are to be performed on PET, as it may already lead to polymer chain breakings subsequently added to those induced in the plasma environment.

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Plasma-treated PET surfaces improve the biocompatibility of human endothelial cells

Cited by 116 publications

References 14 publications

Active screen plasma nitriding enhances cell attachment to polymer surfaces

Active screen plasma nitriding enhances cell attachment to polymer surfaces

Nitrogen‐Rich Plasma‐Polymerized Coatings on PET and PTFE Surfaces Improve Endothelial Cell Attachment and Resistance to Shear Flow

Low Pressure Radio Frequency Ammonia Plasma Surface Modification on Poly(ethylene terephthalate) Films and Fibers: Effect of the Polymer Forming Process

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