Plasma α2-HS glycoprotein concentrations in patients with acute myocardial infarction quantified by a modified ELISA

Mathews, Suresh T.; Deutsch, Diane D.; Iyer, Gayethri; Hora, Nivedita; Pati, Biswajit; Marsh, James D.; Grunberger, George

doi:10.1016/s0009-8981(02)00013-x

Cited by 21 publications

(12 citation statements)

References 28 publications

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“…Regarding its major physiological function, fetuin-A has, however, been unequivocally shown to inhibit pathological calcification at the systemic level (35) and to regulate bone mineralization (39). As previously reported, fetuin-A is the human homologue of bovine fetuin, with an approximate molecular weight of 60 kDa (1,21,24,42). Human fetuin-A has a two-chain form whose N-terminal heavy chain is disulfide bonded to the C-terminal light chain.…”

Section: Discussionmentioning

confidence: 90%

“…Nearly 20% of the circulating fetuin-A pool is phosphorylated at serine-120 and serine-130 (15). Fetuin-A synthesis is down-regulated significantly during injury, infection, inflammation, and malignancy (21,24). The promoter for fetuin-A has been characterized, and its down-regulation during the acute phase of infection has been attributed to transcriptional inactivation (3,13).…”

Section: Discussionmentioning

confidence: 99%

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Fetuin-A, a Hepatocyte-Specific Protein That BindsPlasmodium bergheiThrombospondin-Related Adhesive Protein: a Potential Role in Infectivity

et al. 2005

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Malaria infection is initiated when the insect vector injectsPlasmodium sporozoites into a susceptible vertebrate host. Sporozoites rapidly leave the circulatory system to invade hepatocytes, where further development generates the parasite form that invades and multiplies within erythrocytes. Previous experiments have shown that the thrombospondin-related adhesive protein (TRAP) plays an important role in sporozoite infectivity for hepatocytes. TRAP, a typical type-1 transmembrane protein, has a long extracellular region, which contains two adhesive domains, an A-domain and a thrombospondin repeat. We have generated recombinant proteins of the TRAP adhesive domains. These TRAP fragments show direct interaction with hepatocytes and inhibit sporozoite invasion in vitro. When the recombinant TRAP A-domain was used for immunoprecipitation against hepatocyte membrane fractions, it bound to ␣2-Heremans-Schmid glycoprotein/ fetuin-A, a hepatocyte-specific protein associated with the extracellular matrix. When the soluble sporozoite protein fraction was immunoprecipitated on a fetuin-A-adsorbed protein A column, TRAP bound this ligand. Importantly, anti-fetuin-A antibodies inhibited invasion of hepatocytes by sporozoites. Further, onset of malaria infection was delayed in fetuin-A-deficient mice compared to that in wild-type C57BL/6 mice when they were challenged with Plasmodium berghei sporozoites. These data demonstrate that the extracellular region of TRAP interacts with fetuin-A on hepatocyte membranes and that this interaction enhances the parasite's ability to invade hepatocytes.Malaria is a deadly parasitic disease which affects nearly 40% of the world's population. In any given year 300 to 500 million clinical cases occur, with more than a million deaths. Malaria is caused by species of the protozoan parasite Plasmodium. The infection is initiated when a female Anopheles mosquito injects Plasmodium sporozoites into a susceptible vertebrate host. The sporozoites migrate to the liver, where they undergo many rounds of schizogony in the hepatocytes. The resulting merozoites then invade and multiply within erythrocytes, causing the clinical symptoms of malaria.Understanding the pathogenesis of malaria requires a keen understanding of the molecular mechanisms used by Plasmodium to recognize and invade host cells. Invasion is mediated by interactions between specific parasite molecules and complementary ligands on the host cells. Plasmodium has three invasive stages: (i) sporozoites (sporogonic cycle), which invade the salivary glands of the mosquito; (ii) salivary gland sporozoites (exoerythrocytic cycle), which invade hepatocytes in the vertebrate; and (iii) merozoites (erythrocytic cycle), which invade erythrocytes. The invasion of hepatocytes by sporozoites has been known to require two parasite proteins: circumsporozoite protein (CS) and thrombospondin-related adhesive protein (TRAP) (6,28,30,33,34,44). These proteins are conserved in all Plasmodium species examined to date (26,40) and are present on the parasit...

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Fetuin-A, a Hepatocyte-Specific Protein That BindsPlasmodium bergheiThrombospondin-Related Adhesive Protein: a Potential Role in Infectivity

et al. 2005

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“…In fact, circulating AHSG levels decrease in several conditions characterized by tissue damage, infection, inflammation or malignancy. 16,32,[36][37][38] However, other factors may also have contributed to our observation. It has been shown earlier that AHSG enhances the macrophage-mediated phagocytosis of apoptotic cells in vitro by stimulating macropinocytosis.…”

Section: Discussionmentioning

confidence: 99%

Preeclampsia is associated with decreased serum α2-HS glycoprotein (fetuin-A) concentration

et al. 2009

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The purpose of this study was to determine serum a 2 -HS glycoprotein (AHSG) concentration and its diagnostic accuracy in preeclampsia. In this case-control study, the serum C-reactive protein (CRP) and AHSG levels were measured in 93 preeclamptic patients and in 127 healthy pregnant women by immunoturbidimetry and radial immunodiffusion. The serum CRP levels were significantly higher, whereas the serum AHSG concentrations were significantly lower in the preeclamptic group than in the control group (median (25th to 75th percentile), CRP: 6.71 mg l À1 (2.76-12.69) vs. 3.38 mg l À1 (1.69-7.27), respectively; AHSG: 660 lg ml À1 (612-768) vs. 744 lg ml À1 (660-816), respectively; Po0.001 for both). In preeclamptic patients, the serum AHSG concentrations showed significant inverse correlations with systolic blood pressure and serum CRP levels. A low serum AHSG level (p720 lg ml À1 ) was significantly associated with preeclampsia (adjusted odds ratio (95% confidence interval): 3.69 (1.82-7.51); Po0.001). According to the receiver operating characteristic curves, the measurement of serum AHSG concentrations was as accurate as that of serum CRP levels to detect preeclampsia. In conclusion, serum AHSG concentration is decreased and reflects-at least partly-systemic inflammation in preeclampsia.

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“…For example, human 2-HS glycoprotein (AHSG) is involved in important functions such as inhibition of insulin receptor tyrosine kinase activity, inhibition of protease activities and regulation of calcium metabolism and osteogenesis. Mathews et al (2002) reported that AHSG concentrations start to decrease within a few hours after the onset of acute myocardial infarction (AMI) and return to near normal concentrations during the recovery period (5-7 days after AMI). It is possible that TH may have a role in AMI by regulating the AHSG level.…”

Section: Discussionmentioning

confidence: 99%

Plasma protein regulation by thyroid hormone

Kh¹,

Hy²,

Ch³

et al. 2003

Journal of Endocrinology

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Thyroid hormones (THs) regulate growth, development, differentiation and metabolic processes by interacting and activating thyroid hormone receptors (TRs). Although much progress has been made in our understanding of the transcriptional regulation of many TR target genes, little is known of the regulation of plasma protein gene expression by TRs. To investigate the role of TRs in plasma protein expression we used human hepatocellular carcinoma cell lines and carried out cDNA microarray analysis. Our results indicate that several plasma proteins including transferrin, prothrombin, angiotensinogen, haptoglobin, -2-HS-glycoprotein and chain, complement, lipoproteins and fibrinogen are up-regulated by THs. Furthermore, clusterin, -2-macroglobulin precursor, prothymosin and -fetoprotein were found to be down-regulated by THs.Transferrin, an iron-binding protein expressed in all mammals, and mainly synthesized in the liver, was investigated further. Immunoblot and Northern blot analyses revealed that exposure of HepG2-TR 1 sub-lines and HepG2-Neo cells to tri-iodothyronine (T 3 ) induced time-and dose-dependent increases in the abundance of transferrin mRNA and protein, with the extent of these effects correlating with the level of expression of TR 1. Nuclear run-on experiments indicate that this induction is functioning at the transcriptional level. Moreover, cyclohexamide treatment did not eliminate the induction of transferrin by TH. Thus, our results suggest that the induction of transferrin by TH is direct and may in fact be mediated by an as yet unidentified response element in the promoter region.

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Plasma α2-HS glycoprotein concentrations in patients with acute myocardial infarction quantified by a modified ELISA

Cited by 21 publications

References 28 publications

Fetuin-A, a Hepatocyte-Specific Protein That BindsPlasmodium bergheiThrombospondin-Related Adhesive Protein: a Potential Role in Infectivity

Fetuin-A, a Hepatocyte-Specific Protein That BindsPlasmodium bergheiThrombospondin-Related Adhesive Protein: a Potential Role in Infectivity

Preeclampsia is associated with decreased serum α2-HS glycoprotein (fetuin-A) concentration

Plasma protein regulation by thyroid hormone

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