“Plasmalogen-type” cyclic acetals: formation and conformation of the 1,3-dioxanes and 1,3-dioxolanes from 1-O-cis-alk-1′-enyl-sn-glycerols

Baumann, Wolfgang J.; Madson, T.H.; Weseman, B.J.

doi:10.1016/s0022-2275(20)39369-x

Cited by 10 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the earliest MIR spectroscopic applications was to determine the cis/trans conformation of lipids [ 9 ]. MIR techniques were developed to analyse ingredients in milk [ 10 , 11 ] and wine [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Mid-Infrared Imaging Characterization to Differentiate Lung Cancer Subtypes

Kontsek¹,

Pesti²,

Slezsák³

et al. 2022

Pathol. Oncol. Res.

View full text Add to dashboard Cite

Introduction: Lung cancer is the most common malignancy worldwide. Squamous cell carcinoma (SQ) and adenocarcinoma (LUAD) are the two most frequent histological subtypes. Small cell carcinoma (SCLC) subtype has the worst prognosis. Differential diagnosis is essential for proper oncological treatment. Life science associated mid- and near-infrared based microscopic techniques have been developed exponentially, especially in the past decade. Vibrational spectroscopy is a potential non-destructive approach to investigate malignancies.Aims: Our goal was to differentiate lung cancer subtypes by their label-free mid-infrared spectra using supervised multivariate analyses.Material and Methods: Formalin-fixed paraffin-embedded (FFPE) samples were selected from the archives. Three subtypes were selected for each group: 10-10 cases SQ, LUAD and SCLC. 2 μm thick sections were cut and laid on aluminium coated glass slides. Transflection optical setup was applied on Perkin-Elmer infrared microscope. 250 × 600 μm areas were imaged and the so-called mid-infrared fingerprint region (1800-648cm−1) was further analysed with linear discriminant analysis (LDA) and support vector machine (SVM) methods.Results: Both “patient-based” and “pixel-based” approaches were examined. Patient-based analysis by using 3 LDA models and 2 SVM models resulted in different separations. The higher the cut-off value the lower is the accuracy. The linear C-support vector classification (C-SVC) SVM resulted in the best (100%) accuracy for the three subtypes using a 50% cut-off value. The pixel-based analysis gave, similarly, the linear C-SVC SVM model to be the most efficient in the statistical indicators (SQ sensitivity 81.65%, LUAD sensitivity 82.89% and SCLC sensitivity 88.89%). The spectra cut-off, the kernel function and the algorithm function influence the accuracy.Conclusion: Mid-Infrared imaging could be used to differentiate FFPE lung cancer subtypes. Supervised multivariate tools are promising to accurately separate lung tumor subtypes. The long-term perspective is to develop a spectroscopy-based diagnostic tool, revolutionizing medical differential diagnostics, especially cancer identification.

show abstract

Section: Introductionmentioning

confidence: 99%

Mid-Infrared Imaging Characterization to Differentiate Lung Cancer Subtypes

Kontsek¹,

Pesti²,

Slezsák³

et al. 2022

Pathol. Oncol. Res.

View full text Add to dashboard Cite

show abstract

Produkte des reduktiven Abbaus von α‐(Acyloxy)plasmalogenen aus Rindergewebe‐Lipiden mit LiAlH₄

1991

View full text Add to dashboard Cite

Products of Reductive Degradation of a-(Acy1oxy)plasmalogens from Bovine Tissue Lipids If bovine tissue lipids are treated with LiA1H4, two types of ether-en01 acetate structure was synthesized and treated with unexpected products are detectable: 1-Acetylglycerols (homo-LiA1H4. Corresponding derivatives of 1-acylglycerols 2 as well logs of compound 2) and a-hydroxylated glycerol acetals as a-hydroxylated glycerol acetals 7 and 8 were produced, thus (homologs of compounds 7 and 8). This fact was assumed to confirming the existence of a-(acy1oxy)plasmalogens 1 in tisindicate the presence of a-(acy1oxy)plasmalogens 1, an up to sue of natural origin. They are detectable by GC and GC/MS now unknown class of mammalian tissue lipids. To confirm after conversion of free hydroxy groups with diazomethane/ this assumption, the model compound 6 possessing an enol silica gel into the corresponding methyl ether derivatives.Kurzlich zeigten wir, daD maskierte, d. h. gebundene langkettige a-Hydroxyaldehyde nach Aufarbeitung von Rindergewebe-Lipiden als Dithiolanderivate nachweisbar sind I,*). Wir kamen zu dem SchluD, daD diese a-Hydroxyaldehyde aus a-(Acy1oxy)plasmalogenen 1 freigesetzt werden. In dieser Arbeit berichten wir uber weitere Befunde, die fur das Vorkommen von a-(Acy1oxy)plasmalogenen 1 in Rinderorgan-Lipiden sprechen. Unterzieht man Rinderleber-Lipid einer reduktiven Aufarbeitung mit LiA1H43.4), trennt entstandene Fettalkohole dunnschichtchromatographisch ab, setzt die Restprobe mit CH,N2/Kieselge15) zu Methoxyderivaten um und fraktioniert die Produkte erneut durch DC, so findet man mit GC/MS in Proben einer Fraktion (chromatographische Daten siehe exp. Teil) 1 -Glycerolfettsaureester-Derivate 24). Detektierbar sind ausschlieDlich homologe Verbindungen der Struktur 2 mit vornehm- OCH3

show abstract

Separation and Determination of the Structure of Acylglycerols and Their Ether Analogues

Myher

1978

Fatty Acids and Glycerides

View full text Add to dashboard Cite

“Plasmalogen-type” cyclic acetals: formation and conformation of the 1,3-dioxanes and 1,3-dioxolanes from 1-O-cis-alk-1′-enyl-sn-glycerols

Cited by 10 publications

References 31 publications

Mid-Infrared Imaging Characterization to Differentiate Lung Cancer Subtypes

Mid-Infrared Imaging Characterization to Differentiate Lung Cancer Subtypes

Produkte des reduktiven Abbaus von α‐(Acyloxy)plasmalogenen aus Rindergewebe‐Lipiden mit LiAlH₄

Separation and Determination of the Structure of Acylglycerols and Their Ether Analogues

Contact Info

Product

Resources

About

“Plasmalogen-type” cyclic acetals: formation and conformation of the 1,3-dioxanes and 1,3-dioxolanes from 1-O-cis-alk-1′-enyl-sn-glycerols

Cited by 10 publications

References 31 publications

Mid-Infrared Imaging Characterization to Differentiate Lung Cancer Subtypes

Mid-Infrared Imaging Characterization to Differentiate Lung Cancer Subtypes

Produkte des reduktiven Abbaus von α‐(Acyloxy)plasmalogenen aus Rindergewebe‐Lipiden mit LiAlH4

Separation and Determination of the Structure of Acylglycerols and Their Ether Analogues

Contact Info

Product

Resources

About

Produkte des reduktiven Abbaus von α‐(Acyloxy)plasmalogenen aus Rindergewebe‐Lipiden mit LiAlH₄