Separation and Determination of the Structure of Acylglycerols and Their Ether Analogues

Myher, J. J.

doi:10.1007/978-1-4684-2565-9_3

Cited by 23 publications

(3 citation statements)

References 164 publications

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“…For measuring changes in intracellular free AA, samples were mixed with 0.1/xg nonradioactive AA (for visualization of the AA band with iodine vapor) and were developed in System I:petroleum ether:diethyl ether: acetic acid (vol/vol/vol, 80 : 20 : 1) [46]. For separation of phospholipid, eicosanoids, and neutral lipid, a modification of the one-dimensional, double development system of Heape et al [25] was used.…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

Regulation of intracellular free arachidonic acid inAplysia nervous system

Carlson

Levitan

1990

J. Membrain Biol.

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We have studied the regulation of arachidonic acid (AA) uptake, metabolism, and release in Aplysia nervous system. Following uptake of [3H]AA, the distribution of radioactivity in intracellular and extracellular lipid pools was measured as a function of time in the presence or absence of exogenous AA. The greatest amount of AA was esterified into phosphatidylinositol (relative to pool size). We found that the intracellular free AA pool underwent rapid turnover, and that radioactive free AA and eicosanoids were released at a rapid rate into the extracellular medium, both in the presence and absence of exogenous AA. Most of the released radioactivity originated from phosphatidylinositol. Two pharmacological agents were found to modulate AA metabolism in Aplysia ganglia. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate, stimulated liberation of AA from phosphatidylinositol and phosphatidylcholine. This resulted in an increase in free internal and secreted AA, an increase in conversion of AA to eicosanoids, and an increase in esterification of AA into triacylglycerol. The half maximal dose for TPA-stimulated AA turnover was 15 nM, and the stimulation was dependent on the presence of extracellular calcium. 4-bromophenacylbromide inhibited the redistribution of radioactivity from phospholipid into triacylglycerol, indicating BPB was acting as a phospholipase inhibitor in Aplysia as it does in other systems. These pharmacological agents, in addition to providing information about the regulation of AA metabolism and release, are useful tools for investigating the physiological function of the rapid turnover of AA in Aplysia nervous system.

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Section: Thin-layer Chromatographymentioning

confidence: 99%

Regulation of intracellular free arachidonic acid inAplysia nervous system

Carlson

Levitan

1990

J. Membrain Biol.

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“…Fatty acids were then removed by extraction into trimethylphenylammonium hydroxide (TMPH) (28) leaving the fatty alcohols in the hexane phase. The hexane phase was dried, acetylated (22), and analyzed by GLC. Fatty alcohol acetates were not detected.…”

Section: L-alkyl2-acyl-sn-glycero-3-(2-aminoethyl)phosphonate (Pne)mentioning

confidence: 99%

The Glyceryl Ethers of Paramecium Phospholipids and Phosphonolipids1

Kaneshiro

Meyer

Rhoads

1987

The Journal of Protozoology

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Two glyceryl ethers, 1-O-hexadecyl glycerol and 1-O-cis-octadec-11-enyl glycerol, chimyl and paramecyl alcohol, respectively, were quantified in total phospholipids and five glycerophospholipid classes from cells and cilia of the ciliated protozoon, Paramecium tetraurelia. The ether content of 2-aminoethyl phosphonoglycerolipid was 85-90 mole %. Concentrations of ethers were greatest in the ethanolamine phosphonolipids greater than phosphatidylcholines greater than phosphatidylserines greater than phosphatidylethanolamines greater than phosphatidylinositols. The glyceryl ether concentrations in total cellular phospholipids increased with culture age in P. tetraurelia and P. multimicronucleatum cells. The glyceryl ether concentrations in the phospholipids of P. tetraurelia cilia remained constant from mid log to stationary phase of culture growth. Paramecium tetraurelia phospholipid glyceryl ether concentrations were made greater by supplementation of cultures with chimyl alcohol.

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“…Arrows indicate the direction of water flow.The mass spectra of compounds 1, 2, 3, and 4 showed the characteristic appearance of trimethylsilylated monoglycerides(15)(16)(17). The molecular ions of compounds 1 and 2 as well as of 3 and 4 were only of weak abundance and differed from each other in only two mass units (M + 520/522; see Figs 3,4)…”

mentioning

confidence: 99%

Polyunsaturated monoglycerides and a pregnadiene in defensive glands of the water beetleAgabus affinis

Schaaf

Dettner

2000

Lipids

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In addition to the C21 steroid 15alpha-hydroxypregna-4,6-dien-3,20-dione, four 1- or 2-acylated polyunsaturated monoglycerides, 1- or 2-(cis-5,8,11,14-eicosatetraenoyl)-glycerol and 1- or 2-(cis-5,8,11,14,17-eicosapentaenoyl)glycerol were identified as constituents of the prothoracic defensive gland secretion of the dytiscid beetle Agabus affinis by gas chromatography-mass spectrometry of trimethylsilylated gland extracts. In a feeding assay with minnows, synthetic samples of the two 2-acylated monoglycerides showed only a weak activity as a feeding deterrent. For that reason, other possible functions of the monoglycerides are discussed, such as roles as emulsifiers of cannabimimetics.

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Separation and Determination of the Structure of Acylglycerols and Their Ether Analogues

Cited by 23 publications

References 164 publications

Regulation of intracellular free arachidonic acid inAplysia nervous system

Regulation of intracellular free arachidonic acid inAplysia nervous system

The Glyceryl Ethers of Paramecium Phospholipids and Phosphonolipids1

Polyunsaturated monoglycerides and a pregnadiene in defensive glands of the water beetleAgabus affinis

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