Plasmid-borne cadmium resistance genes in Listeria monocytogenes are similar to cadA and cadC of Staphylococcus aureus and are induced by cadmium

Lebrun, Maryse; Audurier, A; Cossart, Pascale

doi:10.1128/jb.176.10.3040-3048.1994

Cited by 136 publications

(113 citation statements)

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“…While analyzing archaeal genomic sequences 6) we noticed that some of the coded proteins had similarity to eubacterial transcription factors, Fur (ferric uptake regulator), 58) or ArsR (arsenical resistance repressor) 59) and CadC (cadmium resistance C gene product). 60) Many archaea survive in extreme environments, where metals or toxic substances are often present. Thus, these archaeal proteins might regulate transcription in order to utilize such substrates or protect the organisms from them.…”

Section: +mentioning

confidence: 99%

Comparison of modes of DNA-binding and bending by archaeal transcription factors, Phr, TrmB, GvpE, GvpD, NrpR, Lrs14 and MDR1

Suzuki¹

2005

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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Abstract:In earlier publications, I have discussed that transcription of the majority of archaeal genes could be regulated by a single type of factors, i.e. homologues of E. coli Lrp and AsnC, now referred to as feast/famine regulatory proteins (FFRPs). Also I have noted that the 13 bp nucleotide sequences recognized by dimers of various FFRPs are in the same arrangement, here referred to as 5-3-5. In the present study it is shown that the above descriptions also apply to the archaeal transcription factors that have been experimentally identified by other groups since then. In promoters regulated by transcription factors, Phr and TrmB, 13 bp elements in the 5-3-5 arrangement are identified. The amino acid sequences of the two proteins resemble those of known FFRPs at positions important for forming 3D structures of a standard (i.e. full length) FFRP. The same is observed with the amino acid sequences of a transcription factor, GvpE, and its modulator GvpD at its N-terminus. Regulation of promoters by Gvps E and D is explained well by assuming that two types of 13 bp elements in the 5-3-5 arrangement are recognized, respectively, by homo-dimers of GvpE and hetero-dimers of Gvps E and D. Another transcription factor, MDR1, like Lrs14, has a DBD of the FFRP type, but lacks the standard assembly domain: an N-demi FFRP. Including another transcription factor, NrpR, characteristics common with known FFRPs are found for all the transcription factors analyzed, consistently with my earlier arguments.

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Section: +mentioning

confidence: 99%

Comparison of modes of DNA-binding and bending by archaeal transcription factors, Phr, TrmB, GvpE, GvpD, NrpR, Lrs14 and MDR1

Suzuki¹

2005

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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“…For instance, CadA and AtHMA4 are efficient cadmium transporters, respectively, in Listeria monocytogenes and in Arabidopsis thaliana (17,18). However, if the detoxification activity of ScYCF1 (or HsMRP1 in humans) is GSH-dependent, cadmium transporters from the P 1b -ATPases family are functional in the absence of GSH.…”

mentioning

confidence: 99%

A Common Highly Conserved Cadmium Detoxification Mechanism from Bacteria to Humans

Prévéral

Gayet

Moldes

et al. 2009

Journal of Biological Chemistry

101

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Cadmium poses a significant threat to human health due to its toxicity. In mammals and in bakers' yeast, cadmium is detoxified by ATP-binding cassette transporters after conjugation to glutathione. In fission yeast, phytochelatins constitute the cosubstrate with cadmium for the transporter SpHMT1. In plants, a detoxification mechanism similar to the one in fission yeast is supposed, but the molecular nature of the transporter is still lacking. To investigate further the relationship between SpHMT1 and its co-substrate, we overexpressed the transporter in a Schizosaccharomyces pombe strain deleted for the phytochelatin synthase gene and heterologously in Saccharomyces cerevisiae and in Escherichia coli. In all organisms, overexpression of SpHMT1 conferred a markedly enhanced tolerance to cadmium but not to Sb(III), AgNO 3 , As(III), As(V), CuSO 4 , or HgCl 2 . Abolishment of the catalytic activity by expression of SpHMT1 K623M mutant suppressed the cadmium tolerance phenotype independently of the presence of phytochelatins. Depletion of the glutathione pool inhibited the SpHMT1 activity but not that of AtHMA4, a P-type ATPase, indicating that GSH is necessary for the SpHMT1-mediated cadmium resistance. In E. coli, SpHMT1 was targeted to the periplasmic membrane and led to an increased amount of cadmium in the periplasm. These results demonstrate that SpHMT1 confers cadmium tolerance in the absence of phytochelatins but depending on the presence of GSH and ATP. Our results challenge the dogma of the two separate cadmium detoxification pathways and demonstrate that a common highly conserved mechanism has been selected during the evolution from bacteria to humans.Cadmium is a trace element, the presence of which in the environment is essentially due to human activities. It is a highly toxic non-biological heavy metal able to enter living cells via transporters usually used for the uptake of essential cations such as calcium, iron, zinc, and so forth (1). The reactivity of cadmium with thiol groups and its ability to displace essential biological metals result in oxidative stress and eventually cell death (2). To cope with cadmium toxicity, living organisms have developed different strategies.In animals, as in the bakers' yeast cytoplasmic cadmium is complexed with the thiol tripeptide glutathione, a general redox regulator (3, 4). Bis(glutathionato)-cadmium complexes (Cd-GS 2 ) 4 are then driven from the cytoplasm to lesser sensitive cellular compartments by dedicated transporters. The prototypical transporter of Cd-GS 2 is the GS-X pump, ScYCF1, in Saccharomyces cerevisiae (5) and, even if still controversial, to a lesser extent HsMRP1 in humans (6). HsMRP1 probably acts as an efflux pump at the plasma membrane, delivering cadmium in the extracellular medium, whereas ScYCF1 allows sequestration of cadmium into the vacuole (5). A study of a deficient Scycf1 strain has shown that it was extremely cadmium-sensitive, pointing to a major role of ScYCF1 in cadmium tolerance and detoxification (5). Additionally, ScYCF1 wa...

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“…Most of the pyrene degradative proteins are encoded by chromosomes. But in contrast Lin and Cai reported the isolation of plasmid from two strains, Bacillus cereus Py5 and Bacillus megaterium Py6 are involved in Pyrene degradation and after these plasmids transformed to E. coli, Nitrogen fixation: Nitrogen fixing prokaryotic organism are unique for having N 2 fixing ability as well as most of them (Blue green algae) are autotrophs [59][60][61][62][63][64]. Nitrogen fixing genes are conserved in chromosomal DNA and plasmids.…”

Section: Properties Of Plasmidmentioning

confidence: 99%

Role of Plasmids in Microbiology

Banu¹,

Prasad²

2017

J Aquac Res Development

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Plasmid-borne cadmium resistance genes in Listeria monocytogenes are similar to cadA and cadC of Staphylococcus aureus and are induced by cadmium

Cited by 136 publications

References 59 publications

Comparison of modes of DNA-binding and bending by archaeal transcription factors, Phr, TrmB, GvpE, GvpD, NrpR, Lrs14 and MDR1

Comparison of modes of DNA-binding and bending by archaeal transcription factors, Phr, TrmB, GvpE, GvpD, NrpR, Lrs14 and MDR1

A Common Highly Conserved Cadmium Detoxification Mechanism from Bacteria to Humans

Role of Plasmids in Microbiology

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