Plasmid dynamics in Vibrio parahaemolyticus strains related to shrimp Acute Hepatopancreatic Necrosis Syndrome (AHPNS)

Theethakaew, Chonchanok; Nakamura, Shota; Motooka, Daisuke; Matsuda, Shigeaki; Kodama, Takao; Chonsin, Kaknokrat; Suthienkul, Orasa; Iida, Tetsuya

doi:10.1016/j.meegid.2017.04.007

Cited by 33 publications

(27 citation statements)

References 39 publications

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“…This plasmid was named pVA1, and sequence analysis showed that it contained homologs of the insecticidal Photorhabdus insect-related (Pir) binary toxin PirA/PirB [ 13 ]. The importance of these two toxins to AHPND was confirmed by subsequent studies [ 14 , 15 , 16 ], and they are now referred to as V. parahaemolyticus PirA/PirB (PirA vp /PirB vp ).…”

Section: Introductionmentioning

confidence: 74%

“…Although AHPND-detection methods that can monitor the shrimps and the environment during cultivation have already been developed [ 7 , 49 , 50 ], there are still no available drugs that can be used in the treatment of AHPND. It has already been clearly established that PirA vp and PirB vp toxins are the main cytotoxic source of AHPND; for example, the deletion/mutation of their pirA vp and pirB vp genes from pVA1 can decrease AHPND severity and reduce the mortality of the shrimps [ 12 , 13 , 15 , 16 ]. Additionally, PirA vp and PirB vp are both secreted proteins [ 13 , 15 ], which means that they could be easily targeted by drugs/inhibitors.…”

Section: Strategies For Designing Drugs To Block the Cytotoxic Effmentioning

confidence: 99%

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Structural Insights into the Cytotoxic Mechanism of Vibrio parahaemolyticus PirAvp and PirBvp Toxins

2017

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In aquaculture, shrimp farming is a popular field. The benefits of shrimp farming include a relatively short grow-out time, high sale price, and good cost recovery. However, outbreaks of serious diseases inflict serious losses, and acute hepatopancreatic necrosis disease (AHPND) is an emerging challenge to this industry. In South American white shrimp (Penaeus vannamei) and grass shrimp (Penaeus monodon), this disease has a 70–100% mortality. The pathogenic agent of AHPND is a specific strain of Vibrio parahaemolyticus which contains PirAvp and PirBvp toxins encoded in the pVA1 plasmid. PirAvp and PirBvp have been shown to cause the typical histological symptoms of AHPND in infected shrimps, and in this review, we will focus on our structural understanding of these toxins. By analyzing their structures, a possible cytotoxic mechanism, as well as strategies for anti-AHPND drug design, is proposed.

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Section: Introductionmentioning

confidence: 74%

Section: Strategies For Designing Drugs To Block the Cytotoxic Effmentioning

confidence: 99%

Structural Insights into the Cytotoxic Mechanism of Vibrio parahaemolyticus PirAvp and PirBvp Toxins

2017

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“…The pirAB -containing region that comprises the homologue counterparts of the pirAB and pirAB -Tn 903 composite transposons was encoded on the plasmid pLA16-2. The pirAB -containing region was almost identical (>99%) to those from the AHPND-related plasmids pVPE61a ( 10 ), pVA1 ( 11 ), and pVPA3-1 ( 1 ) identified in V. parahaemolyticus , plasmid pVHvo ( 12 ) in V. owensii , and plasmid pVCGX1 ( 8 ) in V. campbellii . Similar to the previous report ( 6 ), the pLA16-2 contained a 3-kb Tn 3 -like transposon that is absent in pVCGX1 in V. campbellii 20130629003S01 isolated from China, as well as other plasmids in V. parahaemolyticus from southeast Asia, thus supporting the geographical variations in the virulence plasmid found among AHPND-related Vibrio isolates from Latin America and Asia.…”

Section: Genome Announcementmentioning

confidence: 87%

Complete Genome Sequence of Acute Hepatopancreatic Necrosis Disease-Causing Vibrio campbellii LA16-V1, Isolated from Penaeus vannamei Cultured in a Latin American Country

Ahn

Piamsomboon

Tang³

et al. 2017

Genome Announc

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“…The farms were located around the Gulf of Nicoya and the Central Pacific region of Costa Rica. Each of the farms were visited two times during the study: at the beginning of one production cycle (between January 2017 and June 2018) and 6-7 weeks after stocking, which is when the literature reports that the highest mortalities caused by AHPND occur in farms (Ananda et al, 2017;Theethakaew et al, 2017).…”

Section: Study Area Type and Designmentioning

confidence: 99%

“…Clinical signs in affected shrimps include lethargy, paleness, soft exoskeletons and atrophy of the hepatopancreas, while the intestine is empty or discontinuous (Dong et al, 2017). AHPND affects the farmed shrimp Penaeus vannamei (OIE, 2018), causing mortalities of 100% between 30 and 40 days farming (Ananda et al, 2017;Theethakaew et al, 2017). It was reported for the first time in China in 2009, while in Latin America it was identified for the first time in 2013 in Mexico, and in 2015 in two unspecified countries of Central America, where atypical mortalities occurred during the first 30 days of farming (Han et al, 2015b;Soto-Rodríguez et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei shrimps in Costa Rica

et al. 2020

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Acute hepatopancreatic necrosis disease (AHPND) is a bacterial disease associated to severe mortality in farmed shrimps, and caused by Vibrio parahaemolyticus containing plasmid pVA-1 encoding pirA and pirB toxins. This study investigates the presence of Vibrio spp. carrying plasmid pVA-1 in post larvae and juveniles Penaeus vannamei from farms located in Costa Rica. Moreover, a possible corelation between Vibrio spp. presence, management parameters, and water quality was also investigated. Between 2017 and 2018, post larvae, the first water pumped into ponds, and juvenile shrimp (6 to 7 weeks after stocking) were collected from 15 farms located in the Gulf of Nicoya and the country's Central Pacific region. On the day when the juvenile shrimp were collected, a survey was applied to farmers to obtain information about management conditions, finally physicalchemical parameters of pond water were measured. Plasmidic pVA-1 pirA and pirB genes were detected in hepatopancreas of juvenile shrimp in 5 (33.3%) farms, while Vibrio spp. were found in 6 (40.0%) farms. Sequencing of pVA-1, pirA and pirB genes showed 99-100% similarity to pathogenic Vibrio parahemolyticus XN89 homologous genes identified in Vietnamese shrimps. Statistically significant differences were found in the water volume (p < .03), rate of water replacement (p < .04), and farms disease history (p < .05). A correlation between presence of Vibrio spp. and water quality was not established. The molecular diagnosis of Vibrio spp., the plasmid and the genes encoding toxins that are associated with AHPND are reported for the first time in Costa Rica. Further studies aimed to isolate AHPND-causing Vibrio spp. from ponds, to generate histopathological data, and to establish economic losses due to AHPND mortalities in Penaeus vannamei farms, are needed to clarify the role and pathogenic features of Vibrio spp. in AHPND.

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Plasmid dynamics in Vibrio parahaemolyticus strains related to shrimp Acute Hepatopancreatic Necrosis Syndrome (AHPNS)

Cited by 33 publications

References 39 publications

Structural Insights into the Cytotoxic Mechanism of Vibrio parahaemolyticus PirAvp and PirBvp Toxins

Structural Insights into the Cytotoxic Mechanism of Vibrio parahaemolyticus PirAvp and PirBvp Toxins

Complete Genome Sequence of Acute Hepatopancreatic Necrosis Disease-Causing Vibrio campbellii LA16-V1, Isolated from Penaeus vannamei Cultured in a Latin American Country

Molecular detection of acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei shrimps in Costa Rica

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