Plasmid-mediated extended-spectrum beta-lactamase-producing strains of Enterobacteriaceae isolated from diabetes foot infections in a Brazilian diabetic center

Abstract

We bacteriologically analyzed 156 species of Enterobacteriaceae, isolated from 138 patients with community-acquired diabetic foot ulcers, in a prospective study made at a diabetic center and at the Federal University of Ceará, Brazil, from March, 2000, to November, 2001.The samples were cultured using selective media, and identification, susceptibility tests and detection of plasmidmediated-extended-spectrum-β β β β β-lactamase (ESBL) producing strains were made with conventional and automated methods. The most frequently occurring pathogens were K. pneumoniae (21.2%), Morganella morganii (19.9%) and E. coli (15.4%). High resistance rates were noted for ampicillin, first generation cephalosporin, trimethoprim/sulfamethoxazole, tetracycline, amoxicillin-clavulanic acid and chloramphenicol. ESBL-producing strains were detected in 6% of the patients. Resistance among gram-negative bacteria has become increasingly common, even in community-acquired infections.

Multidrug Resistant Organisms in Diabetic Foot Ulcers-Analysis of Risk Factors and Clinical Outcome

“…Multidrug resistant gram negative organisms isolated include ESBL and AmpC β lactamase producing enterobacteriaceae and multidrug resistant isolates of P. aeruginosa and Acinetobacter boumannii. Several studies conducted showed a progressive increase in the incidence of ESBL producing strain over years 15,16,17 . This constitute a serious threat to current beta lactam therapy leading to treatment failure in diabetic foot infection.…”

Extended spectrum beta lactamase (ESBL) producing<i> <i>Escherichia</i> coli</i> and <i> klebsiella pneumoniae</i> in diabetic foot infection

“…In a study conducted in 2001, the prevalence was only 6% amongst E.coli isolates. [4] Kapil et al, have reported 54.5 % E.coli isolates to be ESBL producers. [5] We report overall 46.51% E.coli and 44.44% K.pneumoniae isolates to be ESBL producers.…”

“…This rate was lower than the rate found among the sexually transmitted diseases (STD) suspected clinic attendees of Argentina (59.7%). [4] Liver disease due to chronic HBV and HCV infection is becoming a leading cause of death among persons with HIV infection worldwide. Therefore, it would be advisable to detect hepatitis virus co-infections in these patients at the earliest.…”

Prevalence of Pathogenic Bacteria Isolated from Surgical Site and Wound Infection among Patients Admitted in some selected Hospitals in Sokoto Metropolis, Nigeria

“…Antibiotic resistance is a type of drug resistance where a microorganism is able to survive exposure to an antibiotic. Certain antibiotic classes are highly associated with colonization of superbugs (Goossens et al, 2005;Motta et al, 2003). Now days, about 70% of the bacteria that cause infections in hospitals are resistant to at least one of the drugs most commonly used for treatment.…”