Plasmid<scp>DNA</scp>Replication

Tolmasky, Marcelo E.; Actis, Luis A.; Crosa, Jorge H.

doi:10.1002/0471250589.ebt166

Cited by 3 publications

(3 citation statements)

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“…Whereas RNA II acts as the primer that initiates duplication of the DNA strands, RNA I is the counter actor that, by binding to RNA II before priming, inhibits this reaction (Tomizawa, 1985;Tomizawa, 1990a). This control mechanism guarantees an appropriate plasmid dosage within the cell, neither inhibiting plasmid replication nor overloading the host's production machineries (for review, see Tomalsky, 1999). It has been noted that there exists an extensive sequence homology between tRNAs and the ColE1 origin of replication, in particular the unpaired loops of RNA I and RNA II.…”

Section: Introductionmentioning

confidence: 99%

Stabilizing plasmid copy number to improve recombinant protein production

Grabherr

Nilsson

Striedner

et al. 2001

Biotech & Bioengineering

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The key objective for recombinant protein production in bacteria is the maximum exploitation of the cell factory's potential, whereby often strong expression vectors are used to increase product yield. If the metabolic load caused by recombinant expression exceeds the host's capacity, the system exhausts itself, resulting in a loss of protein yield. Excessive plasmid replication is observed after inducing recombinant gene expression, which greatly contributes to metabolic overload of the host cell. The transcriptional and translational machineries are extremely overstrained. By abolishing sequence homology between ColE1 RNA I/RNA II and tRNAs, we were able to restore the plasmid's replication control mechanisms and to keep the plasmid copy number constant throughout the culture process, thereby prolonging metabolic activity and productivity of the bacterial expression system. Because the bacterial host cell is not being exploited beyond its tolerable potential with this method, the constancy of the plasmid copy number level throughout the whole period of the bioprocess provides novel strategies for bioprocess optimization.

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Section: Introductionmentioning

confidence: 99%

Stabilizing plasmid copy number to improve recombinant protein production

Grabherr

Nilsson

Striedner

et al. 2001

Biotech & Bioengineering

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“…Plasmids pOAN01 (170,351 bp), pOAN02 (101,491 bp), and pOAN03 (93,589 bp) harbor one or more RepABC and/or RepC replication or replication-partition systems (2,16). Genes known to be related to stabilization factors have also been found among these three plasmids, including genes encoding ParB-like, PilT-type, and toxin-antitoxin plasmid addiction systems.…”

mentioning

confidence: 99%

Genome of Ochrobactrum anthropi ATCC 49188 ^T , a Versatile Opportunistic Pathogen and Symbiont of Several Eukaryotic Hosts

et al. 2011

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Ochrobactrum anthropi is a common soil alphaproteobacterium that colonizes a wide spectrum of organisms and is being increasingly recognized as an opportunistic human pathogen. Potentially life-threatening infections, such as endocarditis, are included in the list of reported O. anthropi infections. These reports, together with the scant number of studies and the organism's phylogenetic proximity to the highly pathogenic brucellae, make O. anthropi an attractive model of bacterial pathogenicity. Here we report the genome sequence of the type strain O. anthropi ATCC 49188, which revealed the presence of two chromosomes and four plasmids.

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“…Thus, it is most probable that the two plasmids cointegrated and disrupted the pIP843 ColE1-type replicon during the process, a desirable outcome because large plasmids are not stable at high copy numbers, a characteristic of plasmids with this type of replicon (12). In conclusion, we identified a conjugative plasmid that can be a specific example of plasmid cointegration that facilitates dissemination of an extended-spectrum ␤-lactamase gene.…”

mentioning

confidence: 75%