Plasmid transfer between soil bacteria

Klingmüller, Walter; Dally, Andreas; Fentner, Christine; Steinlein, Marion

doi:10.1007/978-94-009-1834-4_10

Cited by 26 publications

(17 citation statements)

References 15 publications

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“…The number of bacteria in each class was expressed as a proportion (%) of the total count. The different distributions gave insight into the distribution of r-and K-strategists in each sample, since characteristics of r-strategists include fast growth in response to enrichment, in contrast with K-strategists, which can be characterized by slow growth in response to enrichment [1,14,15]. "Fast growers" were defined as bacteria that produced visibe colonies at 25°C on 0.1-strength TSA within 24 h, or within 48 h on P-1 medium (Pseudomonas selective agar) [13].…”

Section: Measurementsmentioning

confidence: 99%

“…This indicates that bacteria can acquire genes from a common gene pool, rather than by simply sharing a common ancestor [9]. Bacteria may harbor a great variety of mobile genetic elements, such as plasmids, transposons, and insertion elements, that have at least the potential for considerable genetic exchange and also rearrangement of chromosomal genes [3,8,12,14,30]. The most sensitive techniques for bacterial characterization, such as fatty acid extractions [29] and RNA sequencing [21], have indicated that natural microbial populations appear to have an almost limitless variety of species, sub-species, and strains, a variety that will increase with the development of more and more sensitive and sophisticated identification techniques.…”

Section: Introductionmentioning

confidence: 99%

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The use of colony development for the characterization of bacterial communities in soil and on roots

Leij¹,

Whipps²,

Lynch³

1994

Microb Ecol

227

149

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A simple agar plating method for the description of microbial communities is described. This method is based on the quantification of the numbers of bacterial colonies in 6-7 age-based classes as they appear on agar media over a period of 6-10 days. The method can be used to quantify microbial communities in different habitats (roots and soil) and can be related to the ecophysiology of the microbial communities present. Significant differences in distribution patterns were found in time and depth on the roots. In general, as roots matured, the microbial communities changed from one dominated by r-strategists to one that was more distributed towards K-strategists. The soil had the greatest percentage of organisms that could be characterized as K-strategists. The method was also used to compare microbial communities on wheat roots and in soil in both the field and in microcosms in the glasshouse. In general, the method enabled differentiation between r- and K-strategists in environmental samples, something that could not be done using an ecophysiological index (a modification of the Shannon diversity index) or total bacterial numbers alone.

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Section: Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The use of colony development for the characterization of bacterial communities in soil and on roots

Leij¹,

Whipps²,

Lynch³

1994

Microb Ecol

227

149

View full text Add to dashboard Cite

show abstract

“…Van Elsas et al [30,31] reported transfer of plasmid RP4 between donor and recipient strains introduced into non-sterile soil supplemented with bentonite and nutrients. Similarly, Klingmüller [22,23] observed conjugation in non-sterile soil not before the soil was enriched with nutrients (e.g. saccharose, LB medium).…”

Section: Discussionmentioning

confidence: 94%

“…On the whole, better survival of introduced bacteria has been observed in sterile than non-sterile soil [2,11,[18][19][20][21][22][23][24][25].…”

Section: Discussionmentioning

confidence: 99%

Survival, plasmid transfer and impact ofpseudomonas fluorescensintroduced into soil

Kozdrój

1997

Journal of Environmental Science and Health . Part A: Environme

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The survival of donor, recipient and transconjugant strains of Pseudomonas fluorescens, transfer of plasmid RP4 from donor to recipient, and impact of the introduced strains on indigenous microflora was determined in a sandy-loam soil. The introduced donor and recipient strains survived significantly better in sterile than in non-sterile soil where a progressive decline in their numbers with time was observed. In both soils, conjugation and plasmid RP4 transfer from donor to recipient cells was observed during the first 3 days. The transconjugant survived significantly better when it was the only strain inoculated into the soil. When introduced into soil pre-colonized by the recipient strain, the transconjugant was undetectable. The introduced strains caused stimulation of total number of indigenous heterotrophic bacteria and selected physiological groups such as proteolytic, amylolytic and am-1139

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“…The rate of transfer of pEA9 on LB plates was in the range of 10 -4 transconjugants per donor. However, in non-sterilized soil, no exconjugants could be detected [13]. in agriculture, we then began to study survival and plasmid spread for such strains in soil.…”

Section: Introductionmentioning

confidence: 99%

Plasmid transfer in natural soil: a case by case study with nitrogen-fixing Enterobacter

Klingmüller¹

1991

FEMS Microbiology Letters

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Plasmid transfer between soil bacteria

Cited by 26 publications

References 15 publications

The use of colony development for the characterization of bacterial communities in soil and on roots

The use of colony development for the characterization of bacterial communities in soil and on roots

Survival, plasmid transfer and impact ofpseudomonas fluorescensintroduced into soil

Plasmid transfer in natural soil: a case by case study with nitrogen-fixing Enterobacter

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