Plasmodial Aspartyl-tRNA Synthetases and Peculiarities in Plasmodium falciparum

Bour, Tania; Akaddar, Aziza; Lorber, Bernard; Blais, Sébastien P.; Balg, Christian; Candolfi, Ermanno; Frugier, Magali

doi:10.1074/jbc.m109.015297

Cited by 30 publications

(21 citation statements)

References 58 publications

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“…Ref. 20). Measured diffusion coefficients of P. falciparum and H. sapiens AsnRS samples showed that both recombinant enzymes are dispersed at the concentrations used (20 M monomeric protein).…”

Section: Physicochemical Analysis Of P Falciparum and H Sapiens Asnmentioning

confidence: 99%

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Aminoacylation of Plasmodium falciparum tRNAAsn and Insights in the Synthesis of Asparagine Repeats

Filisetti

Théobald-Dietrich

Mahmoudi

et al. 2013

Journal of Biological Chemistry

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Background:Proteins from the malaria parasite Plasmodium contain extensive asparagine repeats. Results: Plasmodium tRNA Asn concentration and in vitro asparaginylation catalytic parameters were analyzed to explain the frequency of asparagine repeats. Conclusion:The requirement for high levels of asparaginylated tRNAs hinders efficient protein translation. Significance: Limiting the availability of asparaginylated tRNAs represents a plausible regulatory mechanism for the production of properly folded proteins.

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Section: Physicochemical Analysis Of P Falciparum and H Sapiens Asnmentioning

confidence: 99%

“…20. The gene coding for P. falciparum TyrRS was amplified by PCR from P. falciparum cDNA, cloned into pQE30, and purified as described for P. falciparum AsnRS.…”

Section: Rna Seq Data Of Aars Mrna Expression In Sexual and Asexual Smentioning

confidence: 99%

Aminoacylation of Plasmodium falciparum tRNAAsn and Insights in the Synthesis of Asparagine Repeats

Filisetti

Théobald-Dietrich

Mahmoudi

et al. 2013

Journal of Biological Chemistry

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“…One early study reported aaRS activity in P. berghei cell-free extracts (89), and genome sequencing later revealed the entire complement of aaRSs in Plasmodium (10,68,88). But to date, cytoplasmic and apicoplast P. falciparum aspartyl-tRNA synthetase (28,29), lysyltRNA synthetase (30,31), and tryptophanyl-tRNA synthetase (32,33) are the only other malarial aaRSs besides PfGluRS to be functionally or structurally characterized.…”

Section: Discussionmentioning

confidence: 99%

“…Mupirocin, for example, is a topical antibiotic already in clinical use, and other aaRS inhibitors such as the boronated antifungal compound AN2690 (27) are being developed. Aminoacylation in malaria parasites had been little studied despite its critical role in parasite biology and potential as a drug target, but studies describing the apicoplast (28) and cytoplasmic aspartyl-tRNA synthetases (29), lysyl-tRNA synthetases (30,31), and tryptophanyl-tRNA synthetases (32,33) were recently published.…”

mentioning

confidence: 99%

A Nondiscriminating Glutamyl-tRNA Synthetase in the Plasmodium Apicoplast

Mailu

Ramasamay

Mudeppa

et al. 2013

Journal of Biological Chemistry

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Background: Plasmodium apicoplast protein synthesis is essential, but few apicoplast tRNA synthetases have been characterized. Results: Apicoplast glutamyl-tRNA synthetase aminoacylates tRNA Glu and tRNA Gln , is sensitive to a bacterial inhibitor, and is essential in blood stages. Conclusion: Formation of apicoplast Gln-tRNAGln is via indirect aminoacylation. Significance: We demonstrate that the apicoplast glutamyl-tRNA synthetase is a potential drug target.

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“…The P. falciparum genome (11) encodes two putative D-AspRS enzymes, one of which (PF3D7_0514300) possesses a predicted N-terminal apicoplast-targeting sequence, suggesting that it is imported into the apicoplast (56,62). The other AspRS (PF3D7_0102900) lacks an apicoplast-targeting signal and is probably cytoplasmic (57,58,80). Similarly, Plasmodium contains two putative AsnRSs as follows: one apicoplast-targeted (PF3D7_0509600) and the other cytoplasmic (PF3D7_0211800) (11,24,25).…”

Section: Discussionmentioning

confidence: 99%