Plasmodium falciparum genotypes in matched peripheral, placental and umbilical cord blood in an area characterised by unstable malaria transmission in eastern Sudan

Fadlelseed, Omar E.; Osman, Maha E.; Shamseldin, Nahla M.; Elhussein, Amar B.; Adam, Ishag

doi:10.1016/j.heliyon.2017.e00326

Cited by 5 publications

(10 citation statements)

References 21 publications

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“…The present study showed no association between the submicroscopic parasitaemia and age and parity. This is consistent with previous studies performed in an area of unstable malaria transmission in Eastern Sudan [21, 25]. Likewise, age and parity were not associated with submicroscopic malaria in an area with intense malaria transmission in Gabon [29], and in Cameron and Malawi [11, 30].…”

Section: Discussionsupporting

confidence: 91%

“…falciparum infections ( P < 0.001). This finding is in line with the previous studies in Sudan on the frequency of submicroscopic infection in the three compartments [12, 25]. Other reports described submicroscopic parasitaemia in different geographical areas and in a systemic review carried out in Africa; the weighted mean of submicroscopic infection was 36% [16].…”

Section: Discussionsupporting

confidence: 91%

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Impact of Submicroscopic Plasmodium falciparum Parasitaemia on Maternal Anaemia and Low Birth Weight in Blue Nile State, Sudan

Omer

Noureldein

Abdelrahim³

et al. 2019

Journal of Tropical Medicine

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The aim of the present study was to investigate the prevalence of submicroscopic infections and to assess its impact on maternal anaemia and low birth weight. A cross-sectional study was carried out with 1149 consented pregnant women who delivered at 3 main hospitals in the Blue Nile State, between January 2012 and December 2015. From a matched maternal peripheral, placental maternal side, and cord blood sample, blood films and dried spots were prepared for microscopic examination and nested polymerase chain reaction (n-PCR), respectively. 107 out of 447 negative blood films were found to have submicroscopic infection detected using n-PCR in any of the three compartments. Placental samples had a significantly higher prevalence (142) of submicroscopic infections compared with the peripheral (6.5%) and cord (8.1%) samples. The mean (SD) of the maternal haemoglobin (Hb) was significantly lower in cases with submicroscopic parasitaemia (10.9 (0.8) vs. 12.1 (0.7) g/dl, P<0.001) compared with those who had no submicroscopic parasitaemia. Submicroscopic malaria infection was associated with anaemia (OR 19.7, (95% CI, 10.3–37.8)). Thirty-eight babies born to women with submicroscopic infections were low birth weight (LBW) and was significantly lower in submicroscopic parasitaemia (2.663 (0.235) vs. 2.926 (0.341) kg, P<0.001). Submicroscopic malaria infection was associated with LBW (OR = 2.7, (95% CI, 1.2–5.6)). There is a high incidence of submicroscopic infections in any of the three compartments regardless of age or parity. Submicroscopic infection is a risk of maternal anaemia and low birth weight in women in this area of high seasonal malaria transmission.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Impact of Submicroscopic Plasmodium falciparum Parasitaemia on Maternal Anaemia and Low Birth Weight in Blue Nile State, Sudan

Omer

Noureldein

Abdelrahim³

et al. 2019

Journal of Tropical Medicine

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“…The 3D7/MSP2 allele was predominant such as reported in the previous report in the same site (Francine et al, 2016). Similar results are observed in Senegal (Jafari-Guemouri et al, 2005), Sudan (Fadlelseed et al, 2017). In Gabon, the predominant allele was FC27/MSP2 (Kassberger et al, 2002, Mayengue et al, 2004.…”

Section: Discussionsupporting

confidence: 89%

“…Several previous studies on the antigenic diversity of P. falciparum matched in peripheral, placental and umbilical cord blood have shown varied results in different country. Some studies have shown a parasitic overlap found for each of these compartments while others have revealed a subpopulation of peripheral parasites sequestered in the placenta (Kassberger et al, 2002;Mayengue et al, 2004;Fadlelseed et al, 2017;Guitard et al, 2010;Cohee et al, 2016). Recent published work conducted in the same area reported 29 MSP-2 alleles in isolates from peripheral blood of pregnant women with asymptomatic P. falciparum infection at antenatal visits, suggesting a wide diversity of the P. falciparum population (Francine et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Sub-microscopic Plasmodium falciparum infections in matched peripheral, placental and umbilical cord blood samples from asymptomatic Congolese women at delivery

Mbouamboua

Koukouikila-Koussounda

Ntoumi

et al. 2019

Acta Tropica

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“…The msp1 type genes were more diverse in peripheral blood compared to placental and cord blood. This data are different from those reported in Sudanese women, showing higher diversity of the gene in the placental blood compartment [ 37 ], but confirm the previous findings suggesting that the profile of P. falciparum strains circulating in the peripheral blood of pregnant women might differ from that found in the placental and cord blood. Previous studies from high malaria transmission settings have reported several strains of P. falciparum circulating in pregnant women among which some were more prevalent in the placental blood in comparison to peripheral blood compartment.…”

Section: Discussioncontrasting

confidence: 83%

Plasmodium falciparum multiplicity of infection and pregnancy outcomes in Congolese women from southern Brazzaville, Republic of Congo

Massamba

Djontu

Vouvoungui

et al. 2022

Malar J

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Background Investigating whether the multiplicity of Plasmodium falciparum infection (MOI) is related to pregnancy outcomes, is of interest in sub-Saharan area where malaria is highly endemic. The present study aimed to characterize the genetic diversity of P. falciparum in women at delivery from Southern Brazzaville, and investigate whether the MOI is associated with maternal anaemia, preterm delivery, or low birth weight. Methods This was a cross sectional study carried out with samples collected between March 2014 and April 2015 from 371 women recruited at delivery at a Health Centre in southern Brazzaville, Republic of Congo. Matched peripheral, placental, and cord blood collected from each of the women at delivery were used for the detection of P. falciparum microscopic and submicroscopic parasitaemia, and parasite DNA genotyping by nested PCR. Results From 371 recruited women, 27 were positive to microscopic malaria parasitaemia while 223 women harboured submicroscopic parasitaemia. All msp-1 block 2 family allelic types (K1, MAD20 and RO33) were observed in all the three compartments of blood, with K1 being most abundant. K1 (with 12, 10, and 08 alleles in the peripheral, placental, and cord blood respectively) and MAD20 (with 10, 09, and 06 alleles in the respective blood compartments) were more diverse compared to RO33 (with 06, 06, and 05 alleles in the respective blood compartments). From the 250 women with microscopic and/or submicroscopic parasitaemia, 38.5%, 30.5%, and 18.4% of peripheral, placental and cord blood sample, respectively, harboured more than one parasite clone, and polyclonal infection was more prevalent in the peripheral blood of women with microscopic parasitaemia (54.5%) compared to those with submicroscopic parasitaemia (36.7%) (p = 0.02). The mean multiplicity of genotypes per microscopic and submicroscopic infection in peripheral blood was higher in anemic women (2.00 ± 0.23 and 1.66 ± 0.11, respectively) than in non-anaemic women (1.36 ± 0.15 and 1.45 ± 0.06, respectively) (p = 0.03 and 0.06). In logistic regression, women infected with four or more clones of the parasite were 9.4 times more likely to be anaemic than women harbouring one clone. This association, however, was only observed with the peripheral blood infection. No significant association was found between the MOI and low birth weight or preterm delivery. Conclusions These results indicate that the genetic diversity of P. falciparum is high in pregnant women from southern Brazzaville in the Republic of Congo, and the multiplicity of the infection might represent a risk for maternal anaemia.

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Plasmodium falciparum genotypes in matched peripheral, placental and umbilical cord blood in an area characterised by unstable malaria transmission in eastern Sudan

Cited by 5 publications

References 21 publications

Impact of Submicroscopic Plasmodium falciparum Parasitaemia on Maternal Anaemia and Low Birth Weight in Blue Nile State, Sudan

Impact of Submicroscopic Plasmodium falciparum Parasitaemia on Maternal Anaemia and Low Birth Weight in Blue Nile State, Sudan

Sub-microscopic Plasmodium falciparum infections in matched peripheral, placental and umbilical cord blood samples from asymptomatic Congolese women at delivery

Plasmodium falciparum multiplicity of infection and pregnancy outcomes in Congolese women from southern Brazzaville, Republic of Congo

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