Plasmon-Based Biofilm Inhibition on Surgical Implants

Miguel, Ignacio de; Prieto, Irene; Albornoz, Arantxa; Sanz, Vanesa; Weis, Christine; Turón, Pau; Quidant, Romain

doi:10.1021/acs.nanolett.9b00187

Cited by 56 publications

(49 citation statements)

References 29 publications

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“…Interestingly, recent studies have demonstrated the formation of ROS at the surface of metallic nanoparticles (63–65). For example, LSPR-based antibacterial treatment with gold nanoparticles has been reported (66–68).…”

Section: Resultsmentioning

confidence: 99%

Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-ResistantPseudomonas aeruginosa

Silva

Petri

Valencia

et al. 2020

Preprint

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17The interaction of metallic nanoparticles with light excites a local surface plasmon resonance 18 (LSPR). This phenomenon enables the transfer of hot electrons to substrates that release 19 Reactive Oxygen Species (ROS). In this context, the present study was aimed at enhancing 20 the antibacterial effect of citrate-covered silver nanoparticles (AgNPs), which already possess 21 excellent antimicrobial properties, via LSPR excitation with visible LED 22 against Pseudomonas aeruginosa, one of the most refractory organisms to antibiotic 23 treatment. The Minimum Inhibitory Concentration (MIC) of AgNPs was 10 μg/ml under dark 24 conditions and 5 μg/ml under light conditions. The combination of light and AgNPs led to 25 100% cell death after 60 minutes. Quantification of ROS via flow cytometry showed that 26 LSPR stimulated AgNPs increased intracellular ROS concentration by 4.8-fold, suggesting 27 that light-exposed AgNPs caused cell death via ROS production. Light exposition caused a 28 small release of silver ions (0.4%) reaching a maximum after 6 hours. This indicates that 29 silver ions play at most a secondary role in P. aeruginosa death. Overall, the results presented 30 here show that LSPR generation from AgNPs by visible light enhances the antimicrobial 31 activity of silver nanoparticles and can be an alternative for the treatment of topic infections 32 caused by antibiotic-resistant bacteria such as P. aeruginosa. 33 34 36 37 Running title: Light-enhanced Ag nanoparticles against P. aeruginosa 38 39 40 42 ability of fine tuning their composition, structure and shape (1). The desired electronic, 43 optical and chemical properties of nanoparticles can be obtained through modern synthetic 44 techniques devised in the past decades (2). Even small variations in one of these parameters 45 may result in new properties that are absent in the analogous bulk material. Due to its high 46 range of controllable features, metallic nanoparticles are now used in many processes, such as 47 48others. 49Silver nanoparticles (AgNPs)(2), exhibit a great potential of use in many areas, due to their 50 relative easy and controlled synthesis (8), ranging from small and simple spheroidal AgNPs 51 (9) to more complex geometries (10, 11) and even asymmetric shapes (12-14) with solid or 52 hollow interiors (15-17). Historrically, silver has been extensively used as an antimicrobial 53 agent. Many different commercial products relying on silver biocidal properties have been 54 produced, most of them in the last 10 years (18, 19). The wide variety of AgNPs as well as 55 the intrinsic properties of metallic silver in relation to biological system and biomolecules 56 (20) fostered the extensive use of AgNPs in medical applications, such as cancer treatment 57 (21-23), drug delivery (24, 25), imaging (26, 27) and antimicrobial treatment (28, 29). 58 P. aeruginosa is a ubiquitous Gram-negative γ-proteobacterium and an opportunistic 59 pathogen associated with nosocomial infections. It also affects patients afflicted by chronic 60 res...

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Section: Resultsmentioning

confidence: 99%

Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-ResistantPseudomonas aeruginosa

Silva

Petri

Valencia

et al. 2020

Preprint

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“…In their clinically relevant investigations on the ZnO‐SQ coated titanium sheets, 48 h incubation with S. aureus culture was carried out. There was a remarkable presence of proliferating biofilms before light treatment; however, 70% biomass reduction could be obtained when the sheet was illuminated under red light for 1 h, suggesting disruption of biofilms due to photoinduced ROS generation by ZnO‐SQ 157. This is foreseen as a promising strategy to be used as antibacterial coatings for superficial or dental implants.…”

Section: Nanomaterial‐integrated Diagnosis Prevention and Therapy Omentioning

confidence: 99%

Evolving Technologies and Strategies for Combating Antibacterial Resistance in the Advent of the Postantibiotic Era

Karaman

Ercan

Bakay

et al. 2020

Adv Funct Materials

131

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The threats posed by the impending "postantibiotic era" have put forward urgent challenges to be overcome by providing new diagnostic and therapeutic regimes for improved diagnosis and treatment of bacterial infections. Antibiotic resistance and incurable bacterial infections are especially important in a society faced with rapid demographic changes. With very few new antibiotics in the drug development pipeline, not being able to match the pace of antimicrobial resistance evolution, developments within other fields such as materials sciences and medical technologies are required to realize innovative antibacterial approaches. This progress report presents recent advances in especially nanotechnology-based approaches and their concomitant use with complementary antibacterial treatments. Synergistically improved antibacterial activity can be reached by considering novel, promising approaches such as photodynamic and photothermal therapy as well as cold atmospheric pressure treatments as complementary strategies to fight against antibacterial resistance. Moreover, this report describes how these novel technologies can be further improved especially by integration of nanomaterials into the currently applied single modal strategies against bacterial infections.

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“…These compounds or materials could sensitize biofilm infections to conventional antibiotics [6,37]. In addition, photosensitizers and plasmonic nanoparticles were used in combination with lasers to eradicate the biofilm via photodynamic and photothermal therapy [38][39][40][41][42][43]. Extracellular polysaccharides are the major component of biofilm matrix [44].…”

Section: Introductionmentioning

confidence: 99%

A smart hydrogel for on-demand delivery of antibiotics and efficient eradication of biofilms

Zhang

Zhou

et al. 2020

Sci. China Mater.

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Biofilm-associated infections are difficult to treat in the clinics because the bacteria embedded in biofilm are ten to thousand times more resistant to traditional antibiotics than planktonic ones. Here, a smart hydrogel comprised of aminoglycoside antibiotics, pectinase, and oxidized dextran was developed to treat local biofilm-associated infections. The primary amines on aminoglycosides and pectinase were reacted with aldehyde groups on oxidized dextran via a pH-sensitive Schiff base linkage to form the hydrogel. Upon bacterial infection, the increased acidity triggers the release of both pectinase and aminoglycoside antibiotics. The released pectinase efficiently degrades extracellular polysaccharides surrounding the bacteria in biofilm, and thus greatly sensitizes the bacteria to aminoglycosides. The smart hydrogel efficiently eradicated biofilms and killed the embedded bacteria both in vitro and in vivo. This study provides a promising strategy for the treatment of biofilm-associated infections.

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Plasmon-Based Biofilm Inhibition on Surgical Implants

Cited by 56 publications

References 29 publications

Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-ResistantPseudomonas aeruginosa

Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-ResistantPseudomonas aeruginosa

Evolving Technologies and Strategies for Combating Antibacterial Resistance in the Advent of the Postantibiotic Era

A smart hydrogel for on-demand delivery of antibiotics and efficient eradication of biofilms

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