Plasmon-coupled microcavity aptasensors for visual and ultra-sensitive simultaneous detection of Staphylococcus aureus and Escherichia coli

A simple and rapid colorimetric detection of Staphylococcus aureus relied on the distance-dependent optical properties of silver nanoparticles

“…15,16 The LOD and the wide dynamic range obtained in this study demonstrated the enhanced analytical performance in comparison with other kinds of biosensors and assays that have been exploited for detecting and quantifying S. aureus , as expressed in Table 1. This sensing method's performance is possibly compared with the values obtained from the strategies for S. aureus detection based on the magneto-DNA nanoparticle system utilizing a miniaturized NMR device, 16 plasmon-coupled microcavity aptasensors, 32 PCR-based method that amplifies the nuc gene 26 and the quantitative detection method utilizing silica-coated magnetic nanostructures and thermophilic helicase-dependent isothermal amplification. 27 Furthermore, the analytical performance of this biosensor is much better than that of assays based on immunomagnetic beads-based ELISA methods, 28 aptamer-based nanosensor for whole cell detection based on salt induced aggregation of silver nanoparticles, 29 direct loop-mediated isothermal amplification assays, 30 and nano-biosensor based on iron nanoparticles.…”

“…Compared to other metallic nanostructure-based DNA conjugate designs for detection of pathogens, 32,[37][38][39] our proposed sensing method that utilize the distance-dependent optical features of silver nanostructures offers the advantages of simplicity, low cost, short time of analysis, ultrahigh sensitivity and ability for on-site detection, demonstrating the potential application of the approach in pathogen monitoring. Furthermore, with an ultrahigh sensitivity of ∼1-5 CFU mL −1 , the suggested nanoplasmonic biosensor is perfect for pathogen detection in low-density samples, and it can be applied for point-of-care settings.…”