Background To explore the feasibility of utilizing polypropylene (PP) and natural rubber (NR) blends as alternative material for rubber dam clamps in dentistry.Methods The hardness of various PP/NR blend ratios compared to the commercial product, SoftClamp™. Selected blend ratios exhibiting hardness values resembling rigid plastic were further evaluated for hardness and tensile strength after undergoing 1, 5, and 10 autoclave cycles.Results One-way ANOVA test results found that PP/NR blends exhibit significantly lower hardness (p < 0.001) than the commercial SoftClamp™. PP/NR ratios of 100/0, 90/10, and 80/20 exhibited hardness levels equivalent to 82%, 80%, and 75% of SoftClamp™, respectively. Two-way ANOVA revealed significant reductions in hardness (p < 0.001) and tensile strength (p < 0.001) with increasing NR content across all autoclave cycles. However, post-autoclave assessments at 1, 5, and 10 cycles demonstrated no statistically significant differences in tensile strength (p = 0.058) and hardness (p = 0.52) for PP/NR ratios of 100/0, 90/10, and 80/20 compared to their pre-autoclave states.Conclusions The reduction of the NR content within PP/NR blends decreases hardness and tensile strength while autoclaving did not significantly affect the hardness and tensile strength of PP/NR blends.