Plasticity of life-cycle, physiological thermal traits and Hsp70 gene expression in an insect along the ontogeny: Effect of temperature variability

Arias, María Belén; Poupin, María Josefina; Lardies, Marco A.

doi:10.1016/j.jtherbio.2011.06.011

Cited by 62 publications

(15 citation statements)

References 91 publications

(97 reference statements)

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“…insulare suggesting that host-parasitoid population dynamics could shift in favour of DBM should climate change lead to more frequent occurrences of elevated temperatures. Along with previous studies [8,10,31,36,37], our findings further emphasize the need to consider temperature exposure regimes when extrapolating laboratory studies to natural field situations. Such models will be far more informative and predictive if ecologically-relevant local conditions involving gradual ramping to high temperatures are considered.…”

Section: Discussionsupporting

confidence: 82%

“…At the higher extreme temperature, slowly increasing temperature may also allow individuals to continuously express hsp70 at high levels. For example, in Tenebrio molitor L. (Coleoptera: Tenebrionidae) hsp70 gene expression was higher at fluctuating temperatures (-2.5 to 43°C) than under constant temperature (18°C) with the same average temperature [31]. It is also possible that once the adult insect’s extreme threshold temperature is reached, as indicated by the increased mortality, the effect on insect physiology is different than at the lower extreme temperature.…”

Section: Discussionmentioning

confidence: 99%

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Survival and Hsp70 Gene Expression in Plutella xylostella and Its Larval Parasitoid Diadegma insulare Varied between Slowly Ramping and Abrupt Extreme Temperature Regimes

et al. 2013

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BackgroundIn nature, insects have evolved behavioural and physiological adaptations to cope with short term exposure to extreme temperatures. Extreme heat events may increase as a result of climate change; this in turn will affect insect population dynamics. We examined the effect of abrupt and ecologically relevant gradual exposure to high temperatures on the survival and hsp70 gene expression in diamondback moth (DBM) adults and the parasitoid Diadegma insulare , as well as in parasitized and non-parasitized DBM larvae.Principal FindingsTolerance to high temperatures in DBM adults was higher than in D . insulare adults. There was no difference in the survival of DBM adults between abrupt and ramped increases from 25 to 38°C; however, at 40°C survival was higher when the temperature increased gradually. In contrast, more D . insulare adults survived when the temperature was ramped rather than shifted abruptly to both 38 and 40°C. There was no heat stress effect of up to 40°C on the survival of either parasitized or non-parasitized DBM larvae. In adults of both species, more hsp70 expression was observed when temperatures increased abruptly to 38°C compared to ramping. In contrast, at 40°C significantly more expression was found in insects exposed to the ramping rather than the abrupt regime. Hsp70 expression level was in agreement with adult survival data and appears to be a good indicator of stress levels. In parasitized and non-parasitized larvae, hsp70 expression was significantly higher after abrupt shifts compared to ramping at both temperatures.Conclusions/Significance Hsp70 gene expression was responsive to extreme temperatures in both DBM and D . insulare , which may underlie the ability of these insects to survive in extreme temperatures. Survival and hsp70 expression upon abrupt changes are distinctly different from those after ramping indicating that experimental protocol must be considered before extrapolating laboratory results to natural field situations.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Survival and Hsp70 Gene Expression in Plutella xylostella and Its Larval Parasitoid Diadegma insulare Varied between Slowly Ramping and Abrupt Extreme Temperature Regimes

et al. 2013

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“…thermally resilient than larvae (Belén Arias et al, 2011). Such observations broadly agree with a general hypothesis advanced by Pörtner and Farrell, who suggest that the thermotolerance window will increase through the gamete and larval stages to maximum values in juveniles, declining again as sexual maturity is reached (Pörtner and Farrell, 2008).…”

Section: Discussionsupporting

confidence: 89%

Latitudinal comparison of thermotolerance and HSP70 production in F2 larvae of the Greenshell mussel (Perna canaliculus)

Dunphy

Ragg

Collings

2012

Journal of Experimental Biology

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SUMMARYWe report the first measures of thermotolerance (recorded as percentage mortality and induced HSP70 production) for pelagic larvae of three populations of the New Zealand greenshell (green-lipped) mussel Perna canaliculus. Our goal was to determine whether distinct populations of P. canaliculus were more susceptible to predicted climate change than others, and whether such patterns of susceptibility were either genetically controlled (local adaptation of populations) or simply reflect the acclimatory capacity of this species. F2 larvae from three P. canaliculus populations (DʼUrville Island, Banks Peninsula and Stewart Island) were subjected to an acute thermal challenge (3h exposure to a fixed temperature in the range 20-42°C). No latitudinal patterns in either percentage mortality or HSP70 protein production were apparent. For all populations, larval mortality (LT 50 ) was between 32.9 and 33.9°C, with significant amounts of HSP70 induction only occurring in those individuals that experienced temperatures of 40°C or greater. The data presented therefore do not support the hypothesis that genetic adaptation of P. canaliculus to distinct thermal environments will be reflected by a corresponding difference in acute heat tolerance. In fact, the apparently vulnerable veligers show a surprisingly wide thermal safety margin. To develop a comprehensive understanding of the effects of ocean warming upon this species, subsequent studies should consider the impacts of sub-lethal stress upon fitness in addition to chronic thermal challenge and, crucially, the response of sedentary juvenile and adult stages.

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“…Our methods are applicable for broader ecological and evolutionary studies on the thermal response of invertebrates (Belén Arias, Poupin & Lardies ; Chen et al . ; Donohue et al .…”

Section: Discussionmentioning

confidence: 99%

Novel applications of thermocyclers for phenotyping invertebrate thermal responses

et al. 2016

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1 High-throughput genomic methods are increasingly used to investigate invertebrate thermal responses with greater dimensionality and resolution than previously achieved. However, corresponding methods for characterizing invertebrate phenotypes are still lacking. To scale up the characterization of invertebrate thermal responses, we propose a novel use of thermocyclers as temperature-controlled incubators.2 Here, we tested the performance of thermocyclers as incubators and demonstrated the application of this method to efficiently characterize the thermal responses of model and non-model invertebrates.3 We found the thermocyclers performed with high precision, accuracy and resolution under various and fluctuating ambient conditions. We were able to successfully characterize the temperature-dependent development of grasshopper eggs (Warramaba virgo), as well as the effects of fluctuating temperature cycles on the survival of mosquito eggs (Aedes aegypti) and developmental success of Drosophila simulans larvae, all with similar survival rates to conventional methods. 4 Thermocyclers are a general and transferrable means to scale up current methods of incubating small invertebrates. They permit rapid characterization of high-dimensional physiological responses to natural thermal regimes. When combined with existing approaches in thermal and evolutionary biology, these methods will advance our understanding of, and ability to predict, biological adaptations and responses to environmental changes.

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Plasticity of life-cycle, physiological thermal traits and Hsp70 gene expression in an insect along the ontogeny: Effect of temperature variability

Cited by 62 publications

References 91 publications

Survival and Hsp70 Gene Expression in Plutella xylostella and Its Larval Parasitoid Diadegma insulare Varied between Slowly Ramping and Abrupt Extreme Temperature Regimes

Survival and Hsp70 Gene Expression in Plutella xylostella and Its Larval Parasitoid Diadegma insulare Varied between Slowly Ramping and Abrupt Extreme Temperature Regimes

Latitudinal comparison of thermotolerance and HSP70 production in F2 larvae of the Greenshell mussel (Perna canaliculus)

Novel applications of thermocyclers for phenotyping invertebrate thermal responses

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