Plasticizer-assisted bonding of poly(methyl methacrylate) microfluidic chips at low temperature

Duan, Haotian; Zhang, Luyan; Chen, Gang

doi:10.1016/j.chroma.2009.11.018

Cited by 21 publications

(15 citation statements)

References 42 publications

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“…Instead, we employed a plasticiser-assisted bonding approach 22 , which relies on coating the PMMA surface with dibutyl phtalate (DBP), and subsequent bonding under pressure and somewhat elevated temperature. After careful optimisation of the amount of DBP/isopropanol solution applied to the surface, devices with good planarity could be produced with high yield.…”

Section: Chip Qualitymentioning

confidence: 99%

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Yılmaz

Utz

2016

Lab Chip

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A compact microfludic device for perfusion culture of mammalian cells under in-situ metabolomic observation by NMR spectroscopy is presented. The chip is made from poly(methyl methacrylate) (PMMA), and uses a poly(dimethyl siloxane) (PDMS) membrane to allow gas exchange. It is integrated with a generic micro-NMR detector developed recently in our group [J. Magn. Reson. 262, 73-80 (2016)]. While PMMA is an excellent material in the context of NMR, PDMS is known to produce strong background signals. To mitigate this, the device keeps the PDMS away from the detection area. The oxygen permeation into the device is quantified using a flow chemistry approach. A solution of glucose is mixed on the chip with one of glucose oxidase, before flowing through the gas exchanger. The resulting concentration of gluconate is measured by 1 H NMR spectroscopy as a function of flow rate. An oxygen equilibration rate constant of 2.4 s −1 is found for the device, easily sufficient to maintain normoxic conditions in a cell culture at modest perfusion flow rates.

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Section: Chip Qualitymentioning

confidence: 99%

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Yılmaz

Utz

2016

Lab Chip

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“…With the use of plasticisers, bonding can be performed at lower temperatures by lowering the glass transition of the material. Low deformation (<10%) low temperature (90 °C) PMMA bonding was achieved using dibutyl phthalate (DBP) as a plasticiser (Duan et al 2010). Nonetheless, DBP is a suspected teratogen banned in many countries, and its presence within the bulk of the final artefact could make the bonding step unsafe and prevent its use in medical applications.…”

Section: Introductionmentioning

confidence: 99%

“…In this work, we show a bonding method that allows for the fast, cost-effective and safe bonding of multilayer stacks of PMMA, thus overtaking part of the barrier that prevents the translation of microfluidics from the laboratory to the real world. The most common PMMA bonding technique is thermal bonding, in which PMMA elements are heated above the glass transition temperature [T g ranging from 85 °C to 165 °C depending on the commercial composition (Duan et al 2010) but usually around 110 °C ] and pressed together. During thermal bonding, the diffusion of polymer chains between the two interfaces leads to the formation of entanglements among the macromolecules (Baldan 2012) and permanent bonding.…”

Section: Introductionmentioning

confidence: 99%

Safe and cost-effective rapid-prototyping of multilayer PMMA microfluidic devices

Liga

Morton

Kersaudy-Kerhoas

2016

Microfluid Nanofluid

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“…The bonding temperature was significantly reduced to 901C based on the fact that DBP can decrease the T g of PMMA [38,39]. After the channel plate was bonded to a cover, a complete microchip could be obtained (Fig.…”

Section: Resultsmentioning

confidence: 99%

A spring‐driven press device for hot embossing and thermal bonding of PMMA microfluidic chips

2010

Self Cite

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A novel spring-driven press device was designed and manufactured for hot embossing and thermal bonding of PMMA microfluidic chips in this work. This simple device consisted of two semi-cylinder silicone rubber press heads, three steel clamping plates, and three compression springs that were assembled together using two screw bolts and two butterfly nuts. The three springs were clamped between the upper and the middle clamping plates, whereas the two press heads were assembled between the middle and the lower clamping plates. After an epoxy template covered by a PMMA plate or a PMMA channel plate together with a cover were sandwiched between two microscopic glass slides for embossing or bonding, respectively, they were clamped between the two elastic press heads of the press device by fastening the screw nuts on the upper clamping plate. Because the convex press heads applied pressure along the middle line of the glass slides, they would deform resulting in a negative pressure gradient from the middle to the sides so that air bubbles between the sandwiched parts could be squeezed out during embossing and bonding processes. High-quality PMMA microfluidic chips were prepared by using this unique device and were successfully applied in the electrophoretic separation of several cations.

show abstract

Plasticizer-assisted bonding of poly(methyl methacrylate) microfluidic chips at low temperature

Cited by 21 publications

References 42 publications

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Safe and cost-effective rapid-prototyping of multilayer PMMA microfluidic devices

A spring‐driven press device for hot embossing and thermal bonding of PMMA microfluidic chips

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