Plastid Molecular Pharming I. Production of Oral Vaccines via Plastid Transformation

Abstract

Herein we review the main biotechnological and immunological aspects of the progress achieved in the field of plastid derived edible vaccines during the last decade. As there is a public debate against genetically modified crops, the advantages and limitations of oral vaccines are also discussed.

Production and downstream processing optimization of plant-made Osmotin and its functional analysis

“…This is the reason that most high-value recombinant protein drugs are not accessible to resource-less populations worldwide 9 . In the last three decades, a large number of biopharmaceuticals have been expressed in plant systems in various plants using transgenic 5,10 , transient 11 , transplastomic 12 , and plant cell culture 13 methods of production. However, in general, every system comes with its own advantages and disadvantages in terms of cost, scalability, safety and production speed, etc.…”

Contributions of the international plant science community to the fight against human infectious diseases – part 1: epidemic and pandemic diseases

“…Transgenic organ cultures such as hairy roots can be regarded as an extension of the cell suspension culture concept because the organ cultures are likewise grown in containment in bioreactors (Doran, 2013 ; Wongsamuth and Doran, 1997 ). Variants on the theme of transgenic plants include transplastomic plants, where the transgene is inserted into the plastid genome rather than the nuclear genome (Bains et al ., 2017 ; Berecz et al ., 2017 ; Bock, 2015 ; Zhang et al ., 2017 ), and rhizosecretion, in which proteins are secreted by the roots of plants into the hydroponic medium, so that aggressive extraction methods are unnecessary (Drake et al ., 2009 ; Madeira et al ., 2016a , b ). The figure includes images from Biorender ( https://biorender.com/ ).…”