Plastination of decalcified bone by a new resin technique

Abstract

Background:The scope of this study was to preserve whole detailed structure of dissected and decalcified bones, taken from used cadavers, by a new plastination technique.Materials and Methods:Specimens we used in this study were sheep femurs and human bones including pelvis, femur, tibia, and fibula. Bones, at first, fixed with 5% formalin and were decalcified with 5% nitric acid, and then were fixed again and washed under the tap water. The resulted flexible bones were dehydrated in −25°C acetone and degreased them in +25°C acetone. Then, the experimental and control specimen were placed in the vacuum chamber for forced impregnation with our new flexible unsaturated polyester resin (UP89 method) and silicon resin (S10 method), respectively. Finally, the strength and flexibility of plastinated decalcified specimens were investigated by tensometer, and the weight diversity was measured by digital balance.Results:Plastinated bones prepared by this technique were found to be dried, non-fragile, durable, odorless, non-greasy, and demonstrating all detailed structures of the bones. Tensile and weight tests results indicated that plastinated decalcified femurs have owned higher flexibility and strength but lesser weight than plastinated undecalcified femurs. The characteristics of both experimental and control groups of plastinated decalcified specimens were found to have no significant difference.Conclusions:Our synthesized resin found to be much more economical than conventional plastination method. In more details, properties of these new products were the same as, S10 method, from points of strength, flexibility and weight, but, since the money cost for producing them was about one fifth that of S10 method.

Evaluation of Decalcification Techniques for Rat Femurs Using HE and Immunohistochemical Staining

“…In order to improve the quality of staining [8], we also neutralized the samples after decalcification with mineral acids. Contrary to the expectation, the sections that were decalcified in 3% or 5% nitric acid were easily to fall off from the slides after neutralization.…”