Platelet 3H-imipramine binding sites in depressed patients and healthy controls: a comparison between morning and afternoon samples

Gentsch, C.; Lichtsteiner, M.; Gastpar, Markus; Gastpar, G.; Feer, H.

doi:10.1016/0165-0327(89)90057-8

Cited by 6 publications

(1 citation statement)

References 25 publications

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“…Galzin et al (1987) found B max was higher in the dark phase in rabbit platelets studied at different times of day over one month. Baron et al (1988), in a re-examination of previously reported data, found no consistent pattern ofcircadian variation in a substantial number of patients studied on a single occasion, and Gentsch et al (1989) found no difference between moming and aftemoon sampIes taken from both depressed and healthy patients on the same day. In contrast to the animal studies, Nankai et al (1986) found a significant circadian variation in binding in six subjects studied over one day, with higher binding levels in the light phase.…”

Section: Samenvattingmentioning

confidence: 93%

Circadian Variation in Platelet Imipramine Binding During the Day in Healthy Subjects

Ellis

R²,

Cooke³

et al. 1991

Pharmacopsychiatry

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Platelet tritiated imipramine binding values in healthy controls vary considerably from study to study. A possible contributor to such variation might be a circadian rhythm affecting binding, although previous studies of this have been contradictory. Platelet imipramine binding was examined in 12 healthy, medication-free subjects studied at 8 a. m., 11 a. m., 4 p. m., and 10 p. m. during one day. Imipramine binding was determined on platelet membranes, using 0.8-8 nM 3H-imipramine, and nonspecific binding was defined by 50 microM desipramine. All samples from a given individual were assayed simultaneously. The intra-assay coefficient of variation was 6.3 percent. There was no evidence of significant differences in binding capacity or affinity (Bmax or Kd) at different times of day. Circadian variation was explored using COSINOR analysis (DeMet et al., 1989). There was no evidence of circadian variation in binding using this model, even when only the variable portion of binding was considered for each individual. Intraindividual variation in binding was substantial, with a mean coefficient of variation of 29 percent for Bmax and 38 percent for Kd. The possible basis of this variation is unclear, but may reflect the presence of "occult" binding sites in the membrane, or the effect of endogenous modulators of binding. The interrelationship of Bmax and Kd may also be a factor. It was considered that low-affinity binding did not account for a significant part of the variation in Kd in this assay. The utility of imipramine binding as a biological marker of depression may be limited by such levels of intraindividual variation in binding parameters.

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Section: Samenvattingmentioning

confidence: 93%

Circadian Variation in Platelet Imipramine Binding During the Day in Healthy Subjects

Ellis

R²,

Cooke³

et al. 1991

Pharmacopsychiatry

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Genetic and environmental influences on behavioral and neurochemical aspects of emotionality in rats

1988

Self Cite

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Three pairings of rats (two derived from divergent, selective breeding and one from divergent environmental conditions) were compared with regard to behavioral and hormonal parameters. Striking differences were observed: results obtained in our own laboratory as well as those found in a review of the literature pointed to higher emotionality (e.g., increased defecation and corticosterone secretion, etc.) in Roman low-avoidance, Wistar-Kyoto and group-housed rats, as compared to their respective counterparts, Roman high-avoidance, spontaneously hypertensive, and individually housed Wistar rats. Concomitant receptor binding studies reviewed here (3H-diazepam- and 3H-imipramine-binding sites) have revealed, however, less consistent intrapair differences.

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Platelet tritiated imipramine binding in patients suffering from mania

Ellis

Mellsop

et al. 1991

Journal of Affective Disorders

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Platelet 3H-imipramine binding sites in depressed patients and healthy controls: a comparison between morning and afternoon samples

Cited by 6 publications

References 25 publications

Circadian Variation in Platelet Imipramine Binding During the Day in Healthy Subjects

Circadian Variation in Platelet Imipramine Binding During the Day in Healthy Subjects

Genetic and environmental influences on behavioral and neurochemical aspects of emotionality in rats

Platelet tritiated imipramine binding in patients suffering from mania

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