Platelet-activating factor-acether is a component of human follicular fluid

The platelet-activating factor (PAF) is a proinflammatory lipid present in the fluid of ovarian Graafian follicle. Ovarian blockage of the PAF receptor (PAFr) reduces ovulations in the rat whereas underlying mechanism is poorly understood. Mural granulosa cells (MGC) were mechanically isolated from the theca interna of bovine periovulatory follicle. The mRNA abundance for PAFr, progesterone receptor and cyclooxygenase-2 were measured by real-time PCR. Cytosolic calcium (Ca 21 ) concentration was assayed by microscopy using Fura-2 AM as indicator, 8-isoprostaglandin F 2a (8-isoPGF 2a ) by an ELISA kit. Fluorescent products arising from intracellular oxidation of hydroethidine (HE) and dihydrorhodamine (DHR) were quantified by flow cytometry. The cells expressed PAFr mRNA and PAFr protein and responded to cPAF (nonhydrolyzable form of PAF) with a pulsating increase in Ca 21 , demonstrating functional PAFr. Elevation of Ca 21 was reversed by WEB-2086, an inverse PAFr agonist. cPAF elevated the level of 8-isoPGF 2a in the medium of MGC cultured with luteinizing hormone (LH). cPAF alone had no significant influence on the oxidation of HE and DHR, or 8-iso-PGF 2a level. In MGC from vital periovulatory follicle, PAF and LH signaling plays an important role in regulating the production of excessive oxidants. Blockage of PAFr seems to interfere with these regulatory processes essential for ovulation. ' 2009 International Society for Advancement of Cytometry

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“…PAF is present in the fluid of human periovulatory Graafian follicles (7). The cultured ovine follicular wall has been shown to secrete PAF (8).…”

Section: Introductionmentioning

confidence: 99%

Platelet‐activating factor‐stimulated production of reactive oxygen species in ovarian granulosa cells from periovulatory follicles

Krüger

Vernunft

et al. 2009

Cytometry Pt A

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“…PAF has been shown to be secreted from the cultured ovine follicular wall and PAF levels increased within 2 hr after the endogeneous pre‐ovulatory LH surge 23 . The detection of PAF in human FF collected near the time of ovulation indicated the local ovarian production of PAF 11 . These observations provide support for the hypothesis that PAF plays a role in the ovulatory mechanism 19 …”

Section: Discussionmentioning

confidence: 62%

“…subsequently reported a decrease in the PAF concentration in rat ovarian tissue within several hours after the administration of human chorionic gonadotrophin (hCG). It has also been reported that PAF is present in human FFs 11 …”

Section: Introductionmentioning

confidence: 81%

The Effects of Platelet‐Activating Factor on the Secretion of Interleukin‐8 and Growth‐Regulated Oncogene α in Human Immortalized Granulosa Cell Line (GC1a)

Kawano

Furukawa

Fukuda

et al. 2007

American J Rep Immunol

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“…Reproductive Tract That PAF is an important mediator in reproductive physiology has been best demonstrated in the female, where PAF levels have been correlated with several events (Frenkel et al, 1996;Fukuda et al, 1996;Minhas et al, 1996). PAF is implicated at several stages from ovulation (Abisogun, 1989;Amiel et al, 1991) all the way to parturition (Zhu et al, 1992). It is secreted by the early embryo (Battye et al, 1991) and is involved in implantation (Angle et al, 1988) and early pregnancy (Spinks and O'Neill, 1988).…”

Section: Paf In Reproduction Overview Of Paf In the Femalementioning

confidence: 99%