The Effects of Platelet‐Activating Factor on the Secretion of Interleukin‐8 and Growth‐Regulated Oncogene α in Human Immortalized Granulosa Cell Line (GC1a)

Kawano, Yasushi; Furukawa, Yoichi; Fukuda, Junichiro; Matsumoto, Harunobu; Yuge, Akitoshi; Narahara, Hisashi

doi:10.1111/j.1600-0897.2007.00527.x

Cited by 7 publications

(9 citation statements)

References 27 publications

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“…On the basis of present observations, IL-8 and GROα in FF are thought to lead to neutrophil chemoattraction and accumulation. As our results also indicated that IL-8 and GROα are regulated by a mechanism involving TNF-α, it is likely that IL-8 and GROα may play an important role in the accumulation of neutrophils and in the subsequent induction of ovulation [ 33 ]. It has been suggested that IL-8 and GROα may be important modulators of preovulatory events, not only by attracting and activating neutrophils that will eventually play a role in timely follicular rupture, but also by stimulating new blood vessel formation for the corpus luteum [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

A possible role for AMP-activated protein kinase activated by metformin and AICAR in human granulosa cells

Kai

Kawano

Yamamoto

et al. 2015

Reprod Biol Endocrinol

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BackgroundWomen with polycystic ovary syndrome (PCOS) are generally insulin- resistant and are consequently often treated with metformin. We investigated the effect of metformin and AICAR on the AMP-activated protein kinase (AMPK) pathway.MethodsWe evaluated the effects of 5-amino-imidazole-4-carboxyamide-1- beta-D-ribofuranoside (AICAR) and metformin on tumor necrosis factor (TNF)-alpha- stimulated chemokine production in human granulosa cells. The phosphorylations of AMPK, I-kappaB, 4E-BP-1, p70S6K were analyzed by western immunoblotting.ResultsAICAR and metformin markedly reduced the IL-8 and GROalpha production induced by TNF-alpha. AICAR and metformin also reduced the TNF-alpha-induced phosphorylation of I-kappaB. The phosphorylations of I-kappaB, 4EBP-1, p70S6K were inhibited via an AMPK-dependent signal transduction.ConclusionsThese results suggest that metformin promotes granulosa cell function by reducing a TNF-alpha- and chemokine-mediated inflammatory reaction through an AMPK-dependent pathway. These finding may have implications for metformin’s actions during the treatment of PCOS with metformin.

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Section: Discussionmentioning

confidence: 99%

A possible role for AMP-activated protein kinase activated by metformin and AICAR in human granulosa cells

Kai

Kawano

Yamamoto

et al. 2015

Reprod Biol Endocrinol

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“…Since our previous results also indicated that IL-8 and GRO-α are upregulated by IL-1α, TNF-α [31] and platelet-activating factor [32] in GC1a, IL-8 and GRO-α may play an important role in the accumulation of leukocytes and subsequent induction of ovulation. Moreover, our data also show that the production of IL- 8 and GRO-α is decreased by treatment with U0126, a MEK inhibitor.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that IL-8 and GRO-α are induced by inflammatory cytokines or a lipid mediator such as platelet-activating factor from GC1a [31,32]. …”

Section: Methodsmentioning

confidence: 99%

The Effects of Epidermal Growth Factor and Transforming Growth Factor-a on Secretion of Interleukin-8 and Growth-Regulated Oncogene-a in Human Granulosa-Lutein Cells

Kawano

Zeineh

Furukawa

et al. 2012

Gynecol Obstet Invest

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Background: In order to investigate the roles of epidermal growth factor (EGF) and transforming growth factor (TGF)-α in ovulation, we studied the production of interleukin (IL)-8 and growth-regulated oncogene (GRO)-α in cultured human granulosa-lutein cells. Methods: Granulosa-lutein cells obtained from the follicular fluids of in vitro fertilization and embryo transfer patients were cultured and treated with EGF, TGF-α, tumor necrosis factor (TNF)-α or 12-O-tetradecanoylphorbol 13-acetate (TPA). An immortalized granulosa cell line (GC1a) was also cultured and treated with EGF, TGF-α or mitogen-activated protein kinase kinase inhibitor. The supernatants were collected, and IL-8 and GRO-α were measured by ELISA. Results: The levels of IL-8 and GRO-α were significantly increased after treatment with EGF, TGF-α, TNF-α and TPA by primary cultured granulosa-lutein cells. The levels of IL-8 and GRO-α were also significantly increased after treatment with EGF or TGF-α in a dose-dependent manner by GC1a. When GC1a was treated with EGF, TGF-α or U0126, the levels of IL-8 and GRO-α were significantly decreased. Conclusion: Our data indicate that the production of IL-8 and GRO-α is upregulated by EGF and TGF-α. It is suggested that EGF and TGF-α may play an important role in luteinization processes involving IL-8 and GRO-α production.

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“…Amounts of basal IL-8 in the ovary are low, but production increases in granulosa cells and theca cells upon LH stimulation [11]. IL-8 is also known to increase vascular permeability [19, 20], which may facilitate the infiltration process. In addition, the infiltrating leukocytes may interact with ovarian endothelial cells and other cell types via a multitude of chemokines during ovulation [21–23].…”

Section: Trafficking Of Leukocytes Into the Ovarymentioning

confidence: 99%

Pituitary–ovary–spleen axis in ovulation

Oakley

Frazer

2011

Trends in Endocrinology & Metabolism

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Leukocytes are rapidly recruited to the preovulatory ovary and play a crucial role as facilitators of ovulation and luteal formation. In this article, recent findings on leukocyte trafficking to the ovary, as well as the physiological role of leukocytes in the ovary, will be summarized and discussed. We then explore the novel hypothesis that the hypothalamus-pituitary-ovarian (HPO) axis might include the spleen as a reservoir of leukocytes by summarizing recent reports on this topic, both in the fields of immunology and reproductive biology.

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The Effects of Platelet‐Activating Factor on the Secretion of Interleukin‐8 and Growth‐Regulated Oncogene α in Human Immortalized Granulosa Cell Line (GC1a)

Abstract: Our data indicated that IL-8 and GROalpha were regulated by C-PAF. The results suggested that PAF may play an important role in human pre-ovulatory processes involving IL-8 and GROalpha production.

Cited by 7 publications

References 27 publications

A possible role for AMP-activated protein kinase activated by metformin and AICAR in human granulosa cells

A possible role for AMP-activated protein kinase activated by metformin and AICAR in human granulosa cells

The Effects of Epidermal Growth Factor and Transforming Growth Factor-a on Secretion of Interleukin-8 and Growth-Regulated Oncogene-a in Human Granulosa-Lutein Cells

Pituitary–ovary–spleen axis in ovulation

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