Platelet activation by human pancreatic fluid

Prinz, Richard A.; Fareed, Jawed; Rock, Alice; Squillaci, Grace; Wallenga, Jeanine

doi:10.1016/0022-4804(84)90195-1

Cited by 12 publications

(10 citation statements)

References 9 publications

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“…Our in vitro data suggest, therefore, that under certain pathophysiological conditions circulating elastase could cause irreversible platelet activation in vivo. The clinical implications of our findings must be carefully investigated, since the process described here may play a role in hemostatic changes observed in patients with septicemia, acute leukemia, pancreatitis (19,35), and possibly other diseases with increased circulating elastase.…”

Section: Discussionmentioning

confidence: 99%

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Exposure of fibrinogen receptors in human platelets by surface proteolysis with elastase.

Kornecki¹,

Ehrlich²,

Mars³

et al. 1986

J. Clin. Invest.

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Human platelets that were preincubated with porcine elastase aggregated spontaneously upon the addition of fibrinogen. Maximal aggregation to fibrinogen was observed with platelets pretreated with an elastase concentration of 111 tg/ml, and halfmaximal aggregation occurred after treatment with 11 ,ug/ml elastase. Binding of radiolabeled fibrinogen to elastase-treated platelets was specific, saturable, and showed a single class of 48,400±9,697 fibrinogen-binding sites per platelet with a dissociation constant of 6.30±1.48 X i0-M. ATP, apyrase, and the stimulators of platelet adenylate cyclase forskolin, prostaglandin El, prostacyclin, and N6, 2'-O-dibutyryl cyclic AMP did not inhibit the fibrinogen-induced aggregation of elastase-treated platelets. EDTA completely blocked the initiation of aggregation and reversed the fibrinogen-induced aggregation of elastasetreated platelets. Monoclonal and polyclonal antibodies directed against glycoproteins (GP) Ilb and II1a completely blocked the fibrinogen-induced aggregation of elastase-treated platelets. Immunoprecipitates with these antibodies obtained from detergent extracts of surface-radiolabeled, intact, and elastase-treated platelets contained the glycoproteins IUb and IIIa. We conclude that surface proteolysis by low concentrations of elastase can expose fibrinogen-binding sites associated with GPIIb and GPIIIa on the platelet surface, resulting in spontaneous aggregation upon the addition of fibrinogen. These findings may be relevant to hemostatic changes observed in patients with increased levels of circulating elastase.

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Section: Discussionmentioning

confidence: 99%

“…The effects of pancreatic proteases on platelet function have been implicated in the development of systemic complications in pancreatitis (35). Proteolysis of platelet surface glycoproteins, in vivo, has been associated with cirrhosis of the liver (36).…”

Section: Discussionmentioning

confidence: 99%

Exposure of fibrinogen receptors in human platelets by surface proteolysis with elastase.

Kornecki¹,

Ehrlich²,

Mars³

et al. 1986

J. Clin. Invest.

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show abstract

“…In rabbits, administration of pancreatic fluid from patients with chronic pancreatitis induced platelet aggregation and activation (Prinz et al, 1984). In cases of acute pancreatitis, platelets have been shown to be activated, and their indices (mean platelet volume, platelet large cell ratio, and platelet distribution width) have also been shown to be elevated between onset and remission of AP (Mimidis et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Coagulation Abnormalities in Acute Pancreatitis

Gould¹,

Mai²,

Liaw³

2012

Pancreatitis - Treatment and Complications

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“…Furthermore, the administration of human pancreatic fluid caused the release of 5HT in parallel with platelet activation [117]. Several studies showed that the production of 5HT can induce further platelet aggregation and 5HT release [118,119], a positive feedback that may lead to thrombus formation [120].…”

Section: Serotoninmentioning

confidence: 99%

Microcirculatory Disturbances in the Pathogenesis of Acute Pancreatitis

Uhlmann¹

2012

Acute Pancreatitis

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Platelet activation by human pancreatic fluid

Cited by 12 publications

References 9 publications

Exposure of fibrinogen receptors in human platelets by surface proteolysis with elastase.

Exposure of fibrinogen receptors in human platelets by surface proteolysis with elastase.

Coagulation Abnormalities in Acute Pancreatitis

Microcirculatory Disturbances in the Pathogenesis of Acute Pancreatitis

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