Platelet–adenovirus vs. inert particles interaction: Effect on aggregation and the role of platelet membrane receptors

Gupalo, Elena; Kuk, Cynthia; Qadura, Mohammad; Бурячковская, Л. И.; Othman, Maha

doi:10.3109/09537104.2012.703792

Cited by 28 publications

(26 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although some CVB interact with the decay‐accelerating factor (DAF) , which is a complement regulatory protein that is expressed commonly on most cell surfaces, including human platelets , our previous studies showing that the CVB3 variant used was not blocked by soluble recombinant human DAF or by blocking antisera, as reported by others , strongly suggest that DAF was not involved. Although we observed a moderate reduction when platelet glycoproteins (GP) α IIb β 3 integrin and GPIb were blocked, the reported association of platelets with many different viruses such as Herpes, Vaccinia, Hanta, Echo, HIV or DENV, points out that platelets can bind viruses in a receptor‐independent way, as has been proven for HCV . Our data did not characterize whether CVB were inside or stuck on the surface.…”

Section: Discussioncontrasting

confidence: 61%

“…Our data did not characterize whether CVB were inside or stuck on the surface. However, the finding of infectious virus in the pellets as well as in the platelet's supernatants allows us to speculate that the open canalicular system might play a role in the harboring and release of CVB, as has been shown for other viruses .…”

Section: Discussionmentioning

confidence: 67%

“…As it has been demonstrated that some viruses induce several platelet activation responses, including P‐selectin, PS and CD40L expression as well as platelet‐leukocyte aggregates, , we next explored the expression of P‐selectin and PS as the main mediators of platelet‐leukocyte interactions and platelet apoptosis. CVB3 binding to platelets resulted in an increased P‐selectin expression and PS exposure levels after 1 hpi (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Up‐regulation of these platelet molecules is not restricted to CVB. For instance, the human adenovirus group C serotype 5 is internalized by platelets in an alphaIIb‐beta3 integrin‐independent manner and induces P‐selectin expression, platelet aggregation and the formation of platelet‐leukocyte aggregates . DENV‐2 exposure can also activate platelets with an increase in P‐selectin expression and fibrinogen‐binding and also induces the apoptosis of platelets via up‐regulation of PS and caspases .…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Platelets interact with Coxsackieviruses B and have a critical role in the pathogenesis of virus‐induced myocarditis

Negrotto

Giusti

Rivadeneyra

et al. 2015

Journal of Thrombosis and Haemostasis

View full text Add to dashboard Cite

Coxsackieviruses B and have a critical role in the pathogenesis of virus-induced myocarditis. J Thromb Haemost 2015; 13: 271-82.See also Koupenova M, Freedman JE. Platelets: the unsung hero of the immune response. This issue, pp 268-70.Summary. Background: To further understand the role of platelets in the pathogenesis of viral infections we explored platelet interaction with Coxsackieviruses B (CVB) 1 and 3. CVB is a group of viruses that cause the majority of human enterovirus-related viral myocarditis; their receptor (CAR) is expressed on the platelet surface and there is a well-characterized CVB3-induced myocarditis murine model. Methods: Human platelets were infected with CVB1 and 3 and viruses were detected in pellets and in supernatants. C57BL/6J mice with or without platelet depletion were inoculated with CVB3 and peripheral blood and heart samples collected at different times postinfection. Results: CVB1 and 3 RNA and a capsid protein were detected in infected platelets. Despite the fact that titration assays in Vero cells showed increasing infectivity titers over time, supernatants and pellets from infected platelets showed similar levels, suggesting that platelets were not susceptible to a replicative infectivity cycle. CVB binding was CAR-independent and resulted in P-selectin and phosphatidylserine (PS) exposure. CVB3-infected mice showed a rapid thrombocytopenia that correlated with an increase in platelet PS exposure and platelet-leukocyte aggregates without modification of platelet P-selectin expression or von Willebrand factor levels. Mortality, viremia, heart viral titers and myocarditis were significantly higher in platelet-depleted than normal animals.Type I IFN levels were not changed but IgG levels were lower in infected and platelet-depleted mice. Conclusions: Our data reveal that platelets play a critical role in host survival and immune response against CVB3 infection.

show abstract

Section: Discussioncontrasting

confidence: 61%

Section: Discussionmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Platelets interact with Coxsackieviruses B and have a critical role in the pathogenesis of virus‐induced myocarditis

Negrotto

Giusti

Rivadeneyra

et al. 2015

Journal of Thrombosis and Haemostasis

View full text Add to dashboard Cite

show abstract

“…22,24 Finally, although different integrin ligands were able to block Ad binding to platelets in vitro, 22 a recent study questioned the role of integrins because blocking aIIbb3 and avb3 integrins does not modify Ad binding and activation of platelets. 25 Studies of Ad-induced thrombocytopenia in different knockout (KO) mouse models helped to identify the role of specific soluble host factors. For example, Ad administration in Von Willebrand factor (VWF) KO mice did not trigger a significant thrombocytopenia compared with wild-type (wt) mice, thereby suggesting a key role of VWF in Ad-associated thrombocytopenia.…”

mentioning

confidence: 99%

Pseudotyping Serotype 5 Adenovirus with the Fiber from Other Serotypes Uncovers a Key Role of the Fiber Protein in Adenovirus 5-Induced Thrombocytopenia

et al. 2016

View full text Add to dashboard Cite

Adenovirus (Ad) infection in humans is associated with inflammatory responses and thrombocytopenia. Although several studies were conducted in mice models to understand molecular and cellular mechanisms of Ad-induced inflammatory responses, only few of them turned their interest toward the mechanisms of Ad-induced thrombocytopenia. Using different depletion methods, the present study ruled out any significant role of spleen, macrophages, and vitamin K-dependent factor in Adinduced thrombocytopenia. Interestingly, mice displaying thrombocytopenia expressed high levels of cytokines/chemokines after Ad administration. Most importantly, pseudotyping adenovirus with the fiber protein from other serotypes was associated with reduction of both cytokine/chemokine production and thrombocytopenia. Altogether, our results suggest that capsid fiber protein (and more precisely its shaft) of Ad serotype 5 triggers the cytokine production that leads to Ad-induced thrombocytopenia.

show abstract

Virus–Platelet Associations

Pryzdial

Lin

Sutherland

2017

Platelets in Thrombotic and Non-Thrombotic Disorders

View full text Add to dashboard Cite

Platelet–adenovirus vs. inert particles interaction: Effect on aggregation and the role of platelet membrane receptors

Cited by 28 publications

References 35 publications

Platelets interact with Coxsackieviruses B and have a critical role in the pathogenesis of virus‐induced myocarditis

Platelets interact with Coxsackieviruses B and have a critical role in the pathogenesis of virus‐induced myocarditis

Pseudotyping Serotype 5 Adenovirus with the Fiber from Other Serotypes Uncovers a Key Role of the Fiber Protein in Adenovirus 5-Induced Thrombocytopenia

Virus–Platelet Associations

Contact Info

Product

Resources

About