Platelet aggregating activity in human embryo culture media free of PAF-acether

Amiel, Marie-Laure; Duquenne, Clotilde; Benveniste, J.; Testart, J

doi:10.1093/oxfordjournals.humrep.a136898

Cited by 29 publications

(7 citation statements)

References 6 publications

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“…However, PAF also has platelet-dependent effects (e.g., platelet aggregation and bronchoconstriction), which have been implicated in some models of anaphylaxis [56]. The sensitivity of murine platelets to PAF is controversial [57][58][59][60]. To explore whether platelet-related effects contributed to ALT, we pretreated mice with FR-49175, an inhibitor of the platelet-dependent actions of PAF [37].…”

Section: Reduction Of Toxicity By Slower Mab Administrationmentioning

confidence: 99%

Monoclonal Antibody–Mediated Toxicity inCryptococcus neoformansInfection: Mechanism and Relationship to Antibody Isotype

Lendvai

Casadevall

1999

J INFECT DIS

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Antibody reagents represent an alternative for the therapy of human cryptococcosis, and monoclonal antibody 18B7 (IgG1) is a candidate for phase I trial in humans with cryptococcosis. However, antibody administration to mice with established Cryptococcus neoformans infection has been reported to produce acute lethal toxicity (ALT). The present study confirmed this phenomenon and investigated the mechanism of ALT. ALT was associated with hemoconcentration, hypotension, and circulatory collapse; however, toxicity could be prevented by platelet-activating factor inhibitor, rat antibody to Fc receptor, or IgM before IgG1. Significant isotype-specific differences were found in ALT, which can be interpreted as consistent with the hypothesis that there are distinct Fc receptors for murine IgG1 and IgG3. The IgM and IgG3 isotype preference in antibody responses to polysaccharide antigens in mice may translate to a lack of toxicity of antigen-antibody complexes during the course of infections with encapsulated pathogens.

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Section: Reduction Of Toxicity By Slower Mab Administrationmentioning

confidence: 99%

Monoclonal Antibody–Mediated Toxicity inCryptococcus neoformansInfection: Mechanism and Relationship to Antibody Isotype

Lendvai

Casadevall

1999

J INFECT DIS

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“…It has been detected using several different methods including a whole-animal bioassay (O'Neill et al, 1987), in vitro bioassays (Collier et al, 1988), radioimmunoassays and mass spectroscopy (Kodama et al, 1989), and has been inde¬ pendently confirmed for the mouse (Adamson et al, 1991) and human (Punjabi et al, 1990) embryo. However, some workers (Amiel et al, 1989;Smal et al, 1990) have failed to demonstrate the release of PAF by embryos. This conflict and a consider¬ ation of its possible causes have been considered in detail in a recent review by O'Neill (1993).…”

Section: Introductionmentioning

confidence: 99%

Detection and preliminary characterization of two enzymes involved in biosynthesis of platelet-activating factor in mouse oocytes, zygotes and preimplantation embryos: dithiothreitol-insensitive cytidinediphosphocholine: 1-O-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase and acetyl-coenzyme A:1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase

Wells

O’Neill²

1994

Reproduction

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The aim of this study was to determine whether the final enzymes in the two biosynthetic pathways for platelet-activating factor (PAF) (the 'de novo' and the 'membrane remodelling' pathways) are present in mouse embryos, zygotes and oocytes. The enzymes are dithiothreitol-insensitive cytidinediphosphocholine: 1-O-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase (cholinephosphotransferase) in the de novo pathway and acetyl-coenzyme A:1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase (acetyltransferase) in the membrane remodelling pathway. Activity of both enzymes was detected in the unfertilized oocyte, the zygote and also in the preimplantation embryo (48, 72 and 96 h after the ovulatory injection of hCG). In both cases the activity was destroyed by boiling and increased linearly with incubation time and the concentration of embryo homogenate present, indicating that the reactions were catalysed by enzymes. The product of the reactions was confirmed as PAF using HPLC and structural analyses by enzymatic digestion. Cholinephosphotransferase required Mg2+ and was inhibited by Ca2+, while acetyltransferase required the presence of NaF (a phosphatase inhibitor). The activity of cholinephosphotransferase was similar in unfertilized oocytes and zygotes, and did not change significantly with advancing developmental stage in preimplantation embryos. Acetyltransferase had a significantly lower specific activity (0.078 +/- 0.044 fmol PAF per oocyte per min, mean +/- SEM) in unfertilized oocytes than in zygotes of corresponding age (0.358 +/- 0.097 fmol PAF per zygote per min) (P < 0.03).(ABSTRACT TRUNCATED AT 250 WORDS)

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“…These include epidermal growth factor [112], insulin-like growth factor [113], heparin-binding epidermal growth factor [114], leukemia inhibitory factor [115], platelet-derived growth factor [116], brain-derived neurotrophic factor [117], platelet activation factor [118] and more recently granulocyte-macrophage colonystimulating factor (GM-CSF) [119]. The addition of GF to media is proposed to be beneficial to embryo growth promoting faster growth, and also act on embryo receptors to reduce apoptosis [120][121][122].…”

Section: Growth Factors In Embryo Culture Mediamentioning

confidence: 99%

Non-Genetic Inheritance, Fertility and Assisted Reproductive Technologies

Zander-Fox¹,

McPherson²,

Lane³

2015

Non-Genetic Inheritance

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The concept of non-genetic inheritance is gaining considerable attention in the assisted reproductive technology (ART) community due to the reported differences between children born from ART and those that are conceived naturally. It has been demonstrated that children conceived via ART have differences in fetal growth, birth weight, congenital abnormalities, cardiometabolic parameters, glucose homeostasis as well as changes to body composition compared to children conceived naturally. Although these changes may have a parental contribution and may be influenced by the pathology of infertility there is concern that the technologies themselves may play a role. In support of this, is emerging evidence that aspects of ART technology such as culture media formulation and insemination method can alter offspring phenotype. In addition it is also documented that exposure to environmental factors, such as toxins can impact on offspring gametogenesis such that these perturbations persist through generations. With the increasing use of ART and the development of new technologies it is vital that we understand whether ART can effect non-genetic inheritance so that we can optimise technology and prevent abnormal programming and its impact on all aspects of offspring health including fertility and a possible transmission to subsequent generations.

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Platelet aggregating activity in human embryo culture media free of PAF-acether

Cited by 29 publications

References 6 publications

Monoclonal Antibody–Mediated Toxicity inCryptococcus neoformansInfection: Mechanism and Relationship to Antibody Isotype

Monoclonal Antibody–Mediated Toxicity inCryptococcus neoformansInfection: Mechanism and Relationship to Antibody Isotype

Non-Genetic Inheritance, Fertility and Assisted Reproductive Technologies

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